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Characterization of calcium deposition induced by Synechocystis sp. PCC6803 in BG11 culture medium |
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| Authors: | Huaxiao Yan Zuozhen Han Hui Zhao Shixue Zhou Naijie Chi Mei Han Xiaoyan Kou Yan Zhang Linlin Xu Chenchen Tian Song Qin |
| Institution: | 1. College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China 2. Shandong Provincial Key Laboratory of Depositional Mineralization and Sedimentary Minerals, College of Geological Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, China 3. State Key Laboratory of Mining Disaster Prevention and Control, Shandong University of Science & Technology, Qingdao, 266590, China 4. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China |
| Abstract: | Calcium carbonate (CaCO3) crystals in their preferred orientation were obtained in BG11 culture media inoculated with Synechocystis sp. PCC6803 (inoculated BG11). In this study, the features of calcium carbonate deposition were investigated. Inoculated BG11 in different calcium ion concentrations was used for the experimental group, while the BG11 culture medium was used for the control group. The surface morphologies of the calcium carbonate deposits in the experimental and control groups were determined by scanning and transmission electron microscopy. The deposits were analyzed by electronic probe micro-analysis, Fourier transform infrared spectrum, X-ray diffraction, thermal gravimetric analysis and differential scanning calorimetry. The results show that the surfaces of the crystals in the experimental group were hexahedral in a scaly pattern. The particle sizes were micrometer-sized and larger than those in the control group. The deposits of the control group contained calcium (Ca), carbon (C), oxygen (O), phosphorus (P), iron (Fe), copper (Cu), zinc (Zn), and other elements. The deposits in the experimental group contained Ca, C, and O only. The deposits of both groups contained calcite. The thermal decomposition temperature of the deposits in the control group was lower than those in the experimental group. It showed that the CaCO3 deposits of the experimental group had higher thermal stability than those of the control group. This may be due to the secondary metabolites produced by the algae cells, which affect the carbonate crystal structure and result in a close-packed structure. The algae cells that remained after thermal weight loss were heavier in higher calcium concentrations in BG11 culture media. There may be more calcium-containing crystals inside and outside of these cells. These results shall be beneficial for understanding the formation mechanism of carbonate minerals. |
| Keywords: | Synechocystis sp PCC6803 preferred orientation biomineralization calcium carbonate thermal stability |
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