| STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ） |
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| 引用本文： | 尤锋,刘静,王新成,徐永立,黄瑞东,张培军.STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ）[J].中国海洋与湖沼学报,2001,19(2):147-151. |
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| 作者姓名： | 尤锋 刘静 王新成 徐永立 黄瑞东 张培军 |
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| 作者单位： | InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 摘 要： | The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied.
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| 关 键 词： | 三倍性 倍数染色体 胚胎发育 早熟 卵 精子 |
| 收稿时间： | 26 September 1999 |
| 修稿时间： | 8 March 2000 |
Study on embryonic development and early growth of triploid and gynogenetic diploid left-eyed flounder,Paralichthys olivaceus (T. et S.) |
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| Feng You,Jing Liu,Xin-cheng Wang,Yong-li Xu,Rui-dong Huang,Pei-jun Zhang.Study on embryonic development and early growth of triploid and gynogenetic diploid left-eyed flounder,Paralichthys olivaceus (T. et S.)[J].Chinese Journal of Oceanology and Limnology,2001,19(2):147-151. |
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| Authors: | Feng You Jing Liu Xin-cheng Wang Yong-li Xu Rui-dong Huang Pei-jun Zhang |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China |
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| Abstract: | The early effects of chromosomal manipulation of eggs and sperm on the yields of triploid and gynogenetic diploid larvae ofParalichthys olivaceus were investigated Triploidy was achieved by cold shocking fertilized eggs at 0–2°C for 45 minutes duration 5 minutes after,
fertilization, and the induced triploidy rates were 31.2%–50% and the relative hatching rates were 53.3%–99%. Gynogenetic
diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0–2°C for 45 minutes duration
5 minutes after fertilization. The induced gynogenetic diploid rates and the relative hatching rates were 94%–96% and 48.5%–68.5%
respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group
was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were
no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the
growth of the gynogenetic diploid experimental group larvae and the control group larvae according to Student’s t-test (α=0.05).
The relationship between the early growth of the triploid experimental group larvae and that of gynogenetic diploid experimental
group larvae was also studied.
Contribution No. 3731 from the Institute of Oceanology, Chinese Academy of Sciences.
Project supported by the “China 863 Program” and Shandong Science and Technology Commission. |
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| Keywords: | triploidy gynogenetic diploid embryonic development early growth P olivaceus |
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