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Characterization,tissue distribution,and expression of neuropeptide Y in olive flounder Paralichthys olivaceus |
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| Authors: | Qian Wang Xungang Tan Shaojun Du Wei Sun Feng You Peijun Zhang |
| Institution: | 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China;University of Chinese Academy of Sciences, Beijing 100049, China 2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China 3. Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland 701 E.Pratt St, Baltimore MD, 21202, USA |
| Abstract: | Neuropeptide Y(NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study,we focused on NPY m RNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY m RNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the-1 h group before feeding,NPY expression in the 3 h group after feeding decreased significantly( P 0.05). Food deprivation led to an 81.7% decrease in NPY m RNA levels in the 24 h fasted group(P 0.05) and a 91.7% decrease in the 48 h fasted group(P 0.05). Therefore,our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture. |
| Keywords: | neuropeptide Y brain fasting olive flounder feeding behavior Paralichthys olivaceus |
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