| Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupiafilicina （Rhodophyta） |
| |
| 引用本文： | 赵吉强,李丽霞.Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupiafilicina （Rhodophyta）[J].中国海洋与湖沼学报,2014,32(6):1364-1372. |
| |
| 作者姓名： | 赵吉强 李丽霞 |
| |
| 作者单位： | College of L(fe Sciences, Yantai University, Yantai 264005, China |
| |
| 基金项目： | Supported by the National Natural Science Foundation of China(No.31300326);the Natural Science Foundation of Shandong Province(Nos.ZR2010DQ007,ZR2011CM006) |
| |
| 摘 要： | Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance.We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase(SOD),peroxidase(POX),catalase(CAT),and ascorbate peroxidase(APX) of red algae Grateloupia filicina(Lamour.) C.Agardh.The activities of SOD,CAT,and APX changed in response to UV-B in a time- and dose-dependent manner.POX activity increased significantly under all three UV-B treatments.The enzymatic assay showed three distinct bands of SODⅠ(Mn-SOD),SODⅡ(Fe-SOD),and SODⅢ(CuZn-SOD) under a low(Luv) and medium(Muv) dose of UV-B irradiation,while SODⅠ and SODⅢ activities decreased significantly when exposed to a high dose of UV-B irradiation(Huv).The activity of POX isoenzymes increased significantly after exposure to UV-B,which is consistent with the total activity.In addition,a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments.Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation.Correspondingly,the daily growth rate declined under all the three exposure doses,and was especially significant under Muv and Huv treatments.These data suggest that,although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage,G.filicina has incomplete tolerance to higher UV-B irradiation stress.
|
| 关 键 词： | UV-B辐射 红藻门 抗氧化酶 抗坏血酸过氧化物酶 POX同工酶 Mn-SOD 超氧化物歧化酶 抗氧化防御系统 |
Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta) |
| |
| Jiqiang Zhao,Lixia Li.Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)[J].Chinese Journal of Oceanology and Limnology,2014,32(6):1364-1372. |
| |
| Authors: | Jiqiang Zhao Lixia Li |
| |
| Institution: | 1. College of Life Sciences, Yantai University, Yantai, 264005, China
|
| |
| Abstract: | Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance. We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ascorbate peroxidase (APX) of red algae Grateloupia filicina (Lamour.) C. Agardh. The activities of SOD, CAT, and APX changed in response to UV-B in a time- and dose-dependent manner. POX activity increased significantly under all three UV-B treatments. The enzymatic assay showed three distinct bands of SODI (Mn-SOD), SODII (Fe-SOD), and SODIII (CuZn-SOD) under a low (Luv) and medium (Muv) dose of UV-B irradiation, while SODI and SODIII activities decreased significantly when exposed to a high dose of UV-B irradiation (Huv). The activity of POX isoenzymes increased significantly after exposure to UV-B, which is consistent with the total activity. In addition, a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments. Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation. Correspondingly, the daily growth rate declined under all the three exposure doses, and was especially significant under Muv and Huv treatments. These data suggest that, although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage, G. filicina has incomplete tolerance to higher UV-B irradiation stress. |
| |
| Keywords: | UV-B irradiation Grateloupia Jilicina antioxidant system isoenzyme electrophoresis |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
|
