Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC |
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| Authors: | Zhang Jingjing Zhang Quanbin Wang Jing Shi Xuelian Zhang Zhongshan |
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| Institution: | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingduo 266071, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | We developed an HPLC method for analysis of the monosaccharide composition of fucoidans.The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid.Using ribose as the internal standard,the monosaccharide derivatives,obtained with 1-Phenyl-3-methyl-5-pyrazolone(PMP),were separated by reverse-phase HPLC using a gradient elution process,and monitored by ultraviolet detection at 245 nm.In the concentration range of 0.1-2.0 mmol/L,the peak area of each monosaccharide had a good linear rel... |
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| Keywords: | fucoidan monosaccharide derivation HPLC |
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