| 海洋土曲霉C23-3与不同类型海洋微生物共培养对其次生代谢产物的影响 |
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| 引用本文： | 梁金月,李文卿,杨静明,刘亚月,聂影影,杨文聪,张翼.海洋土曲霉C23-3与不同类型海洋微生物共培养对其次生代谢产物的影响[J].广东海洋大学学报,2020(1):44-54. |
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| 作者姓名： | 梁金月 李文卿 杨静明 刘亚月 聂影影 杨文聪 张翼 |
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| 作者单位： | ;1.广东海洋大学食品科技学院//广东省水产品加工与安全重点实验室//广东省海洋生物制品工程实验室//广东省海洋食品工程技术研究中心//水产品深加工广东普通高等学校重点实验室//湛江市脑健康海洋药物与营养品重点实验室//广东海洋大学海洋药物研究所;2.广东海洋大学深圳研究院 |
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| 基金项目： | 国家自然科学基金项目(21807015);广东省扬帆计划引进紧缺拔尖人才项目(201433009);广东省自然科学基金(2018A030307046);深圳市科创委基础研究自由探索项目(JCYJ20170306165013264);广东省应用科技研发重大专项(2016B020235001);深圳市大鹏新区产业发展资金(KY20180203及PT201901-05) |
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| 摘 要： | 【目的】研究海洋土曲霉C23-3与不同类型海洋微生物共培养对其次生代谢产物的影响,挖掘土曲霉菌株C23-3的次生代谢潜力。【方法】将副溶血弧菌、枯草芽孢杆菌和胶红酵母的活菌或灭活菌体分别以不同时间与土曲霉C23-3共培养;采用DPPH自由基清除法和Ellman法分别评价各提取物的抗氧化和乙酰胆碱酯酶抑制活性,采用滤纸片法比较各提取物的抗菌活性,采用HPLC指纹图谱分析各提取物化学成分的差异。【结果与结论】发现不同类型的微生物与土曲霉共培养均可激活一些原本沉默的活性次级代谢产物;菌株及共培养物的化学拮抗能力、HPLC指纹图谱中的特征代谢产物变化揭示在活菌培养体系中土曲霉占竞争优势;而且灭活菌体中可能存在某些信号物质,在12 d时对土曲霉群体自感应信号分子丁内酯-I的产生具有比活菌更强的刺激作用。研究结果为深入挖掘海洋真菌代谢潜力及抗老年痴呆等活性先导化合物的发现提供基础。
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| 关 键 词： | 土曲霉 共培养 抗氧化 乙酰胆碱酯酶抑制 抗菌活性 丁内酯-I |
Effects of Co-culture of Marine Fungus Aspergillus terreus C23-3 and Different Types of Marine Microorganisms on Secondary Metabolites |
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| LIANG Jin-yue,LI Wen-qing,YANG Jing-ming,LIU Ya-yue,NIE Ying-ying,YANG Wen-chong,ZHANG Yi.Effects of Co-culture of Marine Fungus Aspergillus terreus C23-3 and Different Types of Marine Microorganisms on Secondary Metabolites[J].Journal of Zhanjiang Ocean University,2020(1):44-54. |
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| Authors: | LIANG Jin-yue LI Wen-qing YANG Jing-ming LIU Ya-yue NIE Ying-ying YANG Wen-chong ZHANG Yi |
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| Institution: | (College of Food Science and Technology,Guangdong Ocean University,Key Laboratory of Guangdong Province Aquatic Products Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Engineering Technology Research Center of Marine Food,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health,Research Institute for Marine Drugs and Nutrition,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Insititute of Guangdong Ocean University,Shenzhen 518120,China) |
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| Abstract: | 【Objective】Study effects of co-culture of marine fungus Aspergillus terreus C23-3 and different types of marine microorganisms on secondary metabolites.Mine secondary metabolic potential of strain Aspergillus terreus C23-3.【Method】The live bacteria or autoclaved biomass of V.parahaemolyticus,B.subtilis and R.mucilaginosa were co-cultured with Aspergillus terreus C23-3 for different times,respectively.The antioxidant and acetylcholinesterase inhibitory activities of the extracts were evaluated using the DPPH method and Ellman method,respectively.The antimicrobial activities of the extracts were compared by paper disk method.The chemical profiles of the extracts were analyzed by HPLC.【Result and Conclusion】It was found that the co-culture of different types of microbes with A.terreus C23-3 could activate some cryptic bioactive secondary metabolites.The chemical antagonistic ability of the strains and co-cultures together with the variation of featured metabolites in the HPLC fingerprints revealed that A.terreus C23-3 was the predominant side in the co-culture systems of live microbes.Furthermore,some signal substance may exist in the autoclaved bacterial biomass,which exhibited stronger stimulus to the production of quorum sensing autoinducing molecule butyrolactone-I by A.terreus C23-3 than live microbes.The above investigation lays the foundation for the in-depth mining the metabolic potential of marine fungi and discovery of bioactive leading compounds for coping with Alzheimer’s disease and other issues. |
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| Keywords: | Aspergillus terreus co-culture antioxidant acetylcholinesterase inhibitory antimicrobial activity butyrolactone-I |
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