| 低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性 |
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| 引用本文： | 邓尚贵,杨燊,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性[J].广东海洋大学学报,2006,26(4):54-58. |
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| 作者姓名： | 邓尚贵 杨燊 秦小明 |
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| 作者单位： | 广东海洋大学食品科技学院,湛江,524025 |
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| 摘 要： | 以南海低值鱼蛋白为原料,采用木瓜蛋白酶和风味酶的复合酶水解得到多肽水解物(HP),并制备多肽-铁(Ⅱ)螯合物,对螯合工艺及螯合产物的结果及抗氧化和抗菌功能特性进行了初步研究。结果表明:1)水解度为5%的多肽与铁(Ⅱ)螯合效果最佳,螯合率为94.15%;2)化学分析及红外光谱表明活性肽与亚铁离子螯合并形成稳定结构的螯合物;(3)经过50%乙醇(体积分数)沉淀的螯合铁具有明显的抗氧化活性,其抗氧化作用相当于维生素E的90%,经过80%乙醇(体积分数)沉淀的螯合物具有显著的抗枯草牙孢杆菌和金黄色葡萄球菌活性,其抑菌圈均直径为13mm。
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| 关 键 词： | 鱼蛋白 酶解 多肽-铁(II)螯合物 抗氧化 抗菌 低值鱼 |
| 文章编号： | 1007-7995(2006)04-0054-05 |
| 修稿时间： | 2006年1月10日 |
Synthesis of Compound Enzymic Peptides Iron from Wastefish and Its Activity of Antioxidation and Antibacterial |
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| DENG Shang-gui,YANG Shen,QIN Xiao-ming.Synthesis of Compound Enzymic Peptides Iron from Wastefish and Its Activity of Antioxidation and Antibacterial[J].Journal of Zhanjiang Ocean University,2006,26(4):54-58. |
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| Authors: | DENG Shang-gui YANG Shen QIN Xiao-ming |
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| Abstract: | This paper focuses on the preparation of wastefish protein chelating Fe~(2+) by compound enzymolysis method,and study on the aspects of chelate condition,antioxidation and antibacterial function.The result shows(1)Chelating Fe~(2+) with the peptides of DH=5% have the best chelation,and the percent of chelation was 94.15%;(2)the yield was explained by analysis chemistry and IR method,the result demonstrated the iron chelate have stable structure;(3)the deposit in the solution of 50% grain alcohol have 90 percent antioxidation of а-tocopherol,the deposit in the solution of 80% grain alcohol showed great inhibitory activity against Bacillus subtilis and Staphylococcus aureus,the antibacterial diameter is 13mm.the above research lays a new way to utilize wastefish and provided the essential information for the enzymatically hydrolyzed wastefish protein chelate. |
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| Keywords: | fish protein enzyme hydrolization compound enzyme peptides-iron chelation enzymic process antioxidation antibacterial wastefish |
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