| 抗草甘磷转基因大豆多重PCR检测及假阳性问题探讨 |
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| 引用本文： | 李信申,何红,胡汉桥,陈国杰,袁琛.抗草甘磷转基因大豆多重PCR检测及假阳性问题探讨[J].广东海洋大学学报,2008,28(6). |
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| 作者姓名： | 李信申 何红 胡汉桥 陈国杰 袁琛 |
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| 作者单位： | [1]广东海洋大学农学院,广东湛江524025 [2]湛江出入境检验检疫局,广东湛江524022 |
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| 基金项目： | 广东省湛江出入境检验检疫局课题资助 |
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| 摘 要： | 以大豆病基因类似物(RGA)基因为对照,用RR大豆中的外源CaMV35S启动子、CP4-EPSPS引物,应用多重PCR方法,从RR大豆中扩增出预期大小的DNA片段。结果表明:将扩增产物回收后测序,经同源性分析扩增产物为CaMV35S启动子和CP4-EPSPS的一部分序列;多重PCR检测的灵敏度为0.08%;PCR检测过程中产生假阳性的原因是污染和非特异性扩增。
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| 关 键 词： | 抗草甘膦 RR大豆 多重PCR |
Multiplex PCR Detection of Transgenetic Soybean Resistant to Glyphosate and Discussion of False Positive Results |
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| LI Xin-shen,HE Hong,HU Han-qiao,CHEN Guo-jie ,YUAN Chen.Multiplex PCR Detection of Transgenetic Soybean Resistant to Glyphosate and Discussion of False Positive Results[J].Journal of Zhanjiang Ocean University,2008,28(6). |
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| Authors: | LI Xin-shen HE Hong HU Han-qiao CHEN Guo-jie YUAN Chen |
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| Institution: | LI Xin-shen1,HE Hong1,HU Han-qiao1,CHEN Guo-jie 2,YUAN Chen 2(1.Agricultural College of Guangdong Ocean University,Zhanjiang 524088,China,2.Zhanjiang Entry-Exit Inspection , Quarantine Bureau,Zhanjiang,524022,China) |
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| Abstract: | It is important to identify the transgenetic plant.The soybean resistance gene analogs(RGA) was used as check,the anticipated fragments were amplified from DNA of transgenetic plant resistant to Glyphosate by PCR method using the primers of CaMV35S promoters,CP4-EPSPS and nested PCR.The fragments were purified from the Agrose gel and sequenced.The homology analysis proved that the fragments were parts of CaMV35S promoters and CP4-EPSPS.The sensitivity of multiplex PCR was 0.08%.The reasons of false positive... |
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| Keywords: | Glyphosate Resistance Transgenic soybean multiplex PCR |
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