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| TCDD对斑马鱼脂质过氧化作用的初步研究 | |
| 引用本文： | 刘连平,聂芳红,孔庆波,林红英,谢英明,杨蓉,陈进军.TCDD对斑马鱼脂质过氧化作用的初步研究[J].广东海洋大学学报,2008,28(1):81-84. |
| 作者姓名： | 刘连平 聂芳红 孔庆波 林红英 谢英明 杨蓉 陈进军 |
| 作者单位： | 1. 广东海洋大学农业生物技术研究所,广东,湛江,524088 2. 广东海洋大学食品科技学院,广东,湛江,524088 3. 中国刑事警察学院替犬技术系,辽宁,沈阳,110034 |
| 基金项目： | 广东省自然科学基金 , 广东省国际科技合作计划 , 广东海洋大学校科研和教改项目 |
| 摘 要： | 将150条斑马鱼随机等分为5组:2,3,7,8-四氯代二苯并二噁英(TCDD)4个染毒组(染毒剂量分别为0.1μg/L、0.2μg/L、0.4μg/L和0.8μg/L)和空白对照组,水质接触染毒5d后,采用分光光度法测定其肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)的活力,研究TCDD对斑马鱼的脂质过氧化作用。结果发现,TCDD各剂量染毒组MDA含量均有所增加,其中0.2μg/L组差异显著(P<0.05),0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组SOD活性均有所下降,其中0.2μg/L、0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组GST活力均降低且差异极显著(P<0.01)。一定剂量的TCDD能引起MDA含量增加,使SOD和GST活力降低,对斑马鱼具有脂质过氧化作用。 |
| 关 键 词： | 斑马鱼 丙二醛 超氧化物歧化酶 谷胱甘肽-S-转移酶 2 3 7 8-四氯代二苯并二噁英 |
| 文章编号： | 1673-9159(2008)01-0081-04 |
| 修稿时间： | 2007年8月10日 |
Studies on Lipid Peroxidation of TCDD in Zebrafish |
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| LIU Lian-ping,NIE Fang-hong,KONG Qing-bo,LIN Hong-ying,XIE Ying-ming,YANG Rong,CHEN Jin-jun.Studies on Lipid Peroxidation of TCDD in Zebrafish[J].Journal of Zhanjiang Ocean University,2008,28(1):81-84. | |
| Authors: | LIU Lian-ping NIE Fang-hong KONG Qing-bo LIN Hong-ying XIE Ying-ming YANG Rong CHEN Jin-jun |
| Abstract: | To study the lipid peroxidation of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in zebrafish, 150 zebrafish were randomly and equally divided into 5 groups: four test groups were exposed to TCDD at 0.1 μg/L, 0.2 μg/L, 0.4 μg/L, 0.8 μg/L , respectively in addition to one control group. Five days after the animals were exposed to the polluted water with different doses of TCDD, respectively, the level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathionetransferase (GST) in liver of zebrafish were determined by means of spectrophotometry. It was found that the MDA level showed some increasing tendency in which 0.2 μg/L of TCDD exposure group was different (P<0.05) and 0.4μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from the control group (P<0.01), that SOD activity showed decreasing tendency and 0.2 μg/L, 0.4 μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from control group (P<0.01), and that GST activity in all TCDD treated groups showed significantly difference from the control group (P<0.01). It wasconcluded that TCDD at certain doses caused hepatic lipid peroxidation in zebrafish by induction of MDA increase and SOD and GST inactivation. |
| Keywords: | zebrafish malondialdehyde superoxide dismutase glutathionetransferase 2 3 7 8-Tetrachlorodibenzo-p-dioxin(TCDD) |
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