| 红鳍笛鲷微卫星DNA标记的开发与遗传多样性分析 |
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| 引用本文： | 郭昱嵩,王中铎,谢子强,刘楚吾.红鳍笛鲷微卫星DNA标记的开发与遗传多样性分析[J].广东海洋大学学报,2011(4):13-17. |
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| 作者姓名： | 郭昱嵩 王中铎 谢子强 刘楚吾 |
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| 作者单位： | 广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室; |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2007BAD29B03); 国家自然科学基金(30972253); 广东省自然科学基金(8452408801001272,10452408801004224) |
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| 摘 要： | 以三亚外海的红鳍笛鲷(Lutjanus erythropterus)自然群体为研究对象,利用磁珠富集法开发19个适用于红鳍笛鲷的微卫星DNA标记,其中16个为多态性标记,等位基因数为2～11,期望杂合度为0.242～0.896,观测杂合度为0.156～1.000,Ler 14、Ler 29、Ler 49等3个位点偏离哈迪-温伯格平衡(P<0.0031),Ler9-Ler18、Ler12-Ler19、Ler46-Ler47、Ler46-Ler49、Ler18-Ler50、Ler14-Ler51等6对位点间检测到明显的连锁(P<0.05),Ler29、Ler13、Ler49、Ler19、Ler24、Ler25、Ler14、Ler51、Ler18、Ler9等10个位点属于高度多态位点多态信息含量(PIC)>0.5],Ler47、Ler12、Ler15、Ler46等4个为中度多态位点(0.25>PIC>0.5)。
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| 关 键 词： | 红鳍笛鲷 微卫星DNA 磁珠富集 遗传多样性 |
Isolation and Genetic Diversity Analysis of Microsatellite DNA in Lutjanus erythopterus |
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| GUO Yu-song,WANG Zhong-duo,XIE Zi-qiang,LIU Chu-wu.Isolation and Genetic Diversity Analysis of Microsatellite DNA in Lutjanus erythopterus[J].Journal of Zhanjiang Ocean University,2011(4):13-17. |
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| Authors: | GUO Yu-song WANG Zhong-duo XIE Zi-qiang LIU Chu-wu |
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| Institution: | GUO Yu-song,WANG Zhong-duo,XIE Zi-qiang,LIU Chu-wu(Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries college,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | 19 microsatellite DNA markers of Lutjanus erythropterus were developed by FIASCO(Fast Isolation by AFLP Sequences Containing repeats)method,of which 16 were polymorphic.The number of alleles per polymorphic locus ranged from 2 to 11 in a sample of 32 individuals captured off the coast of Sanya.Observed and expected heterozygosities per locus varied from 0.156 to 1.000 and from 0.242 to 0.896,respectively.Three loci(Ler 14,Ler 29 and Ler 49) showed significant departure from Hardy-Weinberg equilibrium after ... |
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| Keywords: | Lutjanus erythropterus microsatellite DNA FIASCO genetic diversity |
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