| 花胚胎发育及仔鱼阶段同工酶的初步分析 |
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| 引用本文： | 顾若波,闻海波,徐钢春,张守领.花胚胎发育及仔鱼阶段同工酶的初步分析[J].广东海洋大学学报,2007,27(1):12-16. |
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| 作者姓名： | 顾若波 闻海波 徐钢春 张守领 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心,农业部水生动物遗传育种和养殖生物学重点开放实验室,江苏,无锡,214081 |
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| 基金项目： | 江苏省高技术研究发展计划项目;江苏省无锡市科技计划指导项目 |
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| 摘 要： | <正>鱼类胚胎发育是一个复杂而有序的系统工程,是基因时空综合表达结果。已有的研究证明:机体在生长发育过程中会发生一系列复杂的同工酶时空表达变化,它是基因特异性表达的一种主要作用形式。同工酶研究是研究鱼类个体发育的有力手段之一,
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| 关 键 词： | 花 胚胎发育 仔鱼 同工酶 |
| 文章编号： | 1673-9159（2007）01-0012-05 |
| 修稿时间： | 2006-07-23 |
Analysis on Isozyme of Hemibarbus maculates Bleeker During Embryonic and Larva Development |
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| GU Ruo-bo,Wen Hai-bo,Xu Gang-chun,Zhang Shou-ling.Analysis on Isozyme of Hemibarbus maculates Bleeker During Embryonic and Larva Development[J].Journal of Zhanjiang Ocean University,2007,27(1):12-16. |
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| Authors: | GU Ruo-bo Wen Hai-bo Xu Gang-chun Zhang Shou-ling |
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| Institution: | Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 ,China |
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| Abstract: | The changes of four isozyme of Hemibarbus maculates and its main embryonic and larva development were analyzed by the method of polyacrylamide gel elecrophoresis. The results showed that the activities of EST, LDH and MDH were different at different developmental stages, while SOD was similar. EST isozyme was not detected before hatching and four bands were found during all larva stages with their activities enhanced; the expressions of LDH, MDH were complicated and muhivariant, but stable after Yolk-sac larva stages; only one band was found on SOD isozyme in every developmental periods. The results suggest that EST, LDH and MDH isozyme activities expressed in sequence during embryonic and larvae development, which provides energy substances for the formation of tissues, organs and systems, and guaranties the feeding of larvae. |
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| Keywords: | Hemibarbus maculates embryo development larvae isozyme |
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