|
|||||
|
|
| 似刺鳊鮈发育过程中同工酶变化及组织分布特征分析 | |
| 引用本文： | 闻海波,徐钢春,华丹,张守领,刘晓丹,顾若波.似刺鳊鮈发育过程中同工酶变化及组织分布特征分析[J].广东海洋大学学报,2008,28(1):75-80. |
| 作者姓名： | 闻海波 徐钢春 华丹 张守领 刘晓丹 顾若波 |
| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心农业部水生动物遗传育种和养殖生物学重点开放实验室,江苏,无锡,214081 2. Virginia Polytechnic Institute and State University,VA.24061.USA 3. 南京农业大学无锡渔业学院,江苏,无锡,214081 |
| 基金项目： | 江苏省科技计划 , 中央级公益性科研院所基本科研业务费专项基金 |
| 摘 要： | 运用高pH不连续聚丙烯酰胺凝胶(PAGE)电泳技术分析了似刺鳊鮈胚胎、仔鱼发育过程中5种同工酶变化,并对成鱼的9种组织同工酶分布特征进行了检测。结果发现:除醇脱氢酶(ADH)外,其他4种酶(EST、LDH、MDH、SOD)在发育过程中均被检出,且呈现明显的阶段性特征;在成鱼的4种同工酶也存在明显的组织特异性。4种酶共检测到9个基因位点。 |
| 关 键 词： | 似刺鳊鮈 胚胎发育 仔鱼 同工酶 组织特异性 |
| 文章编号： | 1673-9159(2008)01-0075-06 |
| 修稿时间： | 2007年11月8日 |
Isozyme Changes and Characteristics of Tissue Distribution in the Development of Paracanthobrama guichenoti Bleeker |
|
| WEN Hai-bo,XU Gang-chun,HUA Dan,ZHANG Shou-ling,LIU Xiao-dan,GU Ruo-bo.Isozyme Changes and Characteristics of Tissue Distribution in the Development of Paracanthobrama guichenoti Bleeker[J].Journal of Zhanjiang Ocean University,2008,28(1):75-80. | |
| Authors: | WEN Hai-bo XU Gang-chun HUA Dan ZHANG Shou-ling LIU Xiao-dan GU Ruo-bo |
| Abstract: | The high-pH discontinuous polyacrylamide gel electrophoresis (PAGE) was utilized to analyze changes of the five isozymes in the embryonic and juvenile development periods of Paracanthobrama guichenoti Bleeker, and to measure the distribution characteristics of the specificity of isozymes in nine adult tissues. The research demonstrates: except alcohol dehydrogenase (ADH), all the other four enzymes(EST,LDH,MDH,SOD) have been detected during the period of development, showing marked staggered characteristics. In the four isozymes, there appears marked histological specialty. Nine loci have been detected in the four enzymes. |
| Keywords: | Paracanthobrama guichenoti development of embryo larvae isozyme tissue specificity |
| 本文献已被 维普 万方数据 等数据库收录！ | |