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菠萝天然酶活力测定方法及活力变化
引用本文:谌素华,洪鹏志,章超桦,黄艺宁,于志斌.菠萝天然酶活力测定方法及活力变化[J].广东海洋大学学报,2004,24(4):63-66.
作者姓名:谌素华  洪鹏志  章超桦  黄艺宁  于志斌
作者单位:湛江海洋大学食品科技学院,广东,湛江,524025
摘    要:以菠萝 (AnanascomosusMerr)为原料 ,应用改良的方法对菠萝天然酶活力进行测定 ,对菠萝酶活力变化进行了初步探讨。结果表明 :测定未成熟、成熟菠萝酶活力分别为 10 6 .10和 6 5 .85u/mL ;菠萝肉、皮、整果汁酶活力分别为 86 .98、5 0 .72、5 5 .2 1u/mL ;室温贮藏 ,酶活力取样时为 5 4.97u/mL ,第 4天达最高值 72 .78u/mL ,第 8天降至 4 3.6 6u/mL ;4℃贮藏 ,酶活力取样时为 5 4.97u/mL ,第 2天达最高值71.6 8u/mL ,第 4天降至 5 0 .12u/mL ,即不同温度下贮藏不同时间酶活力变化是先升高后降低

关 键 词:菠萝  天然酶活力  酶活力测定
文章编号:1007-7995(2004)8-0063-04
修稿时间:2003年6月13日

Determination and Inflection Activity of the Crude Enzyme Activity of Pineapple
CHEN Su-hua,HONG Peng-zhi,ZHANG Chao-hua,HUANG Yi-ning,YU Zhi-bin.Determination and Inflection Activity of the Crude Enzyme Activity of Pineapple[J].Journal of Zhanjiang Ocean University,2004,24(4):63-66.
Authors:CHEN Su-hua  HONG Peng-zhi  ZHANG Chao-hua  HUANG Yi-ning  YU Zhi-bin
Abstract:Using pineapple, we applied the simplified method to measure the crude enzyme activity,and discussed the change of enzyme activities of pineapple. The results were summarized as follows: Enzyme activities of immaturity and mature pineapple are 106.10 and 65.85 u/mL, pineapple meat, skin, meat and skin is 86.98, 50.72,55.21 u/mL respectively; In room storage temperature, when samples was taken, enzyme activity is 54.97u/mL , then it goes up the largest 72.78 u/mL at the forth day, to the eighth day it down 43.66u/mL; At 4 ℃ storage temperature enzyme activity is 54.97u/mL in the beginning, and then it goes up the largest 71.68u/mL at the second day, to the forth day it down 50.12 u/mL. That is to say,the activity goes up and then down at different time and temperatures.
Keywords:pineapple  crude enzyme activity  enzyme activity determination
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