| 无定形碳酸钙结合蛋白对鼠前成骨细胞MC3T3-E1碱性磷酸酶活性的影响 |
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| 引用本文： | 万翔,孙娟,苏境坦,梁晓,张文权,郑桂兰,谢莉萍,王洪钟,张贵友,杜晓东,张荣庆.无定形碳酸钙结合蛋白对鼠前成骨细胞MC3T3-E1碱性磷酸酶活性的影响[J].广东海洋大学学报,2012(3):13-18. |
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| 作者姓名： | 万翔 孙娟 苏境坦 梁晓 张文权 郑桂兰 谢莉萍 王洪钟 张贵友 杜晓东 张荣庆 |
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| 作者单位： | 1. 清华大学生命科学学院,北京100084
2. 广东海洋大学水产学院,广东湛江524088 |
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| 基金项目： | 国家自然科学基金(40876068);教育部博士学科点专项科研基金(20090002110055) |
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| 摘 要： | 以 MC3T3-E1细胞系为实验材料,探讨了真核表达的重组蛋白 rACCBP 对成骨细胞的作用.结果表明:rACCBP 对 MC3T3-E1的分化具有抑制作用,用含有0.001 g/mL rACCBP 的 DMEM 培养液,培养8 d 后细胞的ALP 活性仅为7.187 U/mg,远低于参照的11.917 U/mg; rACCBP 削弱了 dexamethane 和 ascorbic acid 对 MC3T3-E1的诱导作用
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| 关 键 词： | 生物矿化 rACCBP MC3T3-E1 碱性磷酸酶 抗坏血酸 地塞米松 |
Effects of Amorphous Calcium Carbonate-Binding Protein on the Alkaline Phosphatase Activity of Mouse Osteoblastic Cell Line MC3T3-E1 |
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| WAN Xiang,SUN Juan,SU Jing-tan,LIANG Xiao,ZHANG Wen-quan,ZHENG Gui-lan,WANG Hong-zhong,DU Xiao-dong,XIE Li-ping,ZHANG Rong-qing.Effects of Amorphous Calcium Carbonate-Binding Protein on the Alkaline Phosphatase Activity of Mouse Osteoblastic Cell Line MC3T3-E1[J].Journal of Zhanjiang Ocean University,2012(3):13-18. |
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| Authors: | WAN Xiang SUN Juan SU Jing-tan LIANG Xiao ZHANG Wen-quan ZHENG Gui-lan WANG Hong-zhong DU Xiao-dong XIE Li-ping ZHANG Rong-qing |
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| Institution: | 1(1.School of Life Sciences,Tsinghua University,Beijing 100084,China;2.Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | Amorphous calcium carbonate binding protein(ACCBP),which is abstracted from a marine mullusc Pinctada fucata,performs notably in biomineralization,in vitro.But its low existence limited the related research.In this research,rACCBP was used,which was expressed in yeast,instead of ACCBP in order to study its functions on MC3T3-E1 cell.First,the reasonable concentration and duration for incubation of rACCBP were studied.rACCBP down-mediated MC3T3-E1 differentiation.Medium with DMEM containing 0.001 μg/mL rACCBP,cultured for 8 days,ALP activity was only 7.187 n mol /min/mg,much lower than 11.917 nm pNPP/min/mg protein.Moreover,interactions between rACCBP,dexamethane and ascorbic acid were studied on three levels,none,low and high.rACCBP also seems to restrain their stimulations on MC3T3-E1 differentiation. |
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| Keywords: | biomineralization rACCBP MC3T3-E1 ALP ascorbic acid dexamethasone |
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