| 腹腔注射恩诺沙星和氟苯尼考对红笛鲷血清IgM含量的影响 |
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| 引用本文： | 张丽敏,吴灶和,简纪常,鲁义善,彭志东,简贺君.腹腔注射恩诺沙星和氟苯尼考对红笛鲷血清IgM含量的影响[J].广东海洋大学学报,2009,29(3):33-37. |
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| 作者姓名： | 张丽敏 吴灶和 简纪常 鲁义善 彭志东 简贺君 |
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| 作者单位： | 广东海洋大学,广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524025 |
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| 摘 要： | 以20mg·kg-1恩诺沙星和氟苯尼考分别腹腔注射红笛鲷,通过间接酶联免疫吸附实验法(ELISA)测定红笛鲷血清IgM的含量,研究恩诺沙星与氟苯尼考对红笛鲷血清IgM含量的影响。结果显示:健康红笛鲷血清中IgM抗体的含量为3.591±0.314mg·mL-1,注射后,恩诺沙星组增加至4.234±0.013mg·mL-1,第3周后恢复至初始水平,而氟苯尼考组则降低至2.538±0.214mg·mL-1,第5周仍未恢复至初始水平。可见,恩诺沙星可提高红笛鲷血清中抗体IgM的含量,而氟苯尼考则降低鱼体血清中抗体IgM的含量,且影响时间也较长。
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| 关 键 词： | 红笛鲷 血清抗体 IgM 恩诺沙星 氟苯尼考 ELISA法 |
Effects of Intraperitonealy Injected Enrofloxacin and Florfenicol on Serum IgM Concentration in Crimson Snapper(Lutjanus sanguineus) |
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| ZHANG Li-min,WU Zao-he,JIAN Ji-chang,LU Yi-shan,PENG Zhi-dong,JIAN He-jun.Effects of Intraperitonealy Injected Enrofloxacin and Florfenicol on Serum IgM Concentration in Crimson Snapper(Lutjanus sanguineus)[J].Journal of Zhanjiang Ocean University,2009,29(3):33-37. |
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| Authors: | ZHANG Li-min WU Zao-he JIAN Ji-chang LU Yi-shan PENG Zhi-dong JIAN He-jun |
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| Abstract: | After injection with 20 mg·kg-1 Enrofloxacin and Florfenicol intraperitonealy,the IgM concentration of Crimson Snapper (Lutjanus sanguineus) were investigated by using indirect ELISA. Results showed that :the concentration of serum antibody IgM of Crimson Snapper is 3.591±0.314 mg·mL-1. Enrofloxacin group can enhance the fish antibody IgM to 4.234±0.013 mg·mL-1. It returned to initial level three weeks later,while the Florfenicom group's was reduced to 2.538±0.214 mg·mL-1,and did not returned to initial level in the fifth week. This shows that Enrofloxacin can increase serum levels of IgM antibody but Florfenicol decrease them and have longer time effects. |
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| Keywords: | IgM |
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