| 野生二粒小麦 Triticum dicoccoides抗杀锈病基因 YrH52分子定位的初步研究(英文) |
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| 引用本文： | PengJ.H.,FahimaT.,RoderM.S.,LiY.C.,DahanA.,GramaA.,NevoE..野生二粒小麦 Triticum dicoccoides抗杀锈病基因 YrH52分子定位的初步研究(英文)[J].广东海洋大学学报,1999(4). |
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| 作者姓名： | PengJ.H. FahimaT. RoderM.S. LiY.C. DahanA. GramaA. NevoE. |
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| 作者单位： | 以色列海法芒特卡梅尔海法大学生物进化研究所!31905(PengJ.H.，FahimaT.，DahanA.，GramaA.)，德国格特斯本科伦兹特西植物遗传与作物研究所!3(RoderM.S.)，D-06466(LiY.C.)，以色列(NevoE.) |
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| 摘 要： | 主要对以色列野生二粒小麦赫尔蒙种群中分离获得的一个抗条锈病基因进行了分子定位研究 ,将源于赫尔蒙山具抗杀锈病的种系 T.dicoccides H52与普通的栽培种 Langdon进行杂交并创建了 F2 代遗传图。研究发现 H52种系抗条锈病的能力由一种显性基因控制 ,将其暂定名为 Yr H52。从 1 2 0个微卫星标记中 ,已经检测到来自亲本 91 %的多态性 ,而且从其中 56个微卫星分子标记中产生了 79个分离的位点 ,有 9个位点显示出了与 Yr H 52基因连锁 ,其重组率 0 .0 2～ 0 .3 5,遗传距离 2 .0 0～ 4 3 .3 7cm之间 ,L OD值 3 .56～ 54.2 2。由 1 0个微卫星位点和 Yr H52构建的染色体 1 B遗传图 ,其图距全长为 1 0 1 .5cm。Yr H52基因位于 Xgwm2 64 a和 Xgwm2 64 c之间 ,且与 Xgwm2 64 a、Xgwm1 8紧密连锁 ,两侧依次分别与 Xgwm1 3 1 a、Xgwm63 6b、Xgwm2 64 c、Xgwm4 0 3 a、Xgwm1 53、Xgwm550 a和 Xgwm1 2 4连锁。同时 ,Yr H52也与 REL P标记物 N or1紧密连锁 ,图距 1 .4 cm,L OD2 9.62。这显然与野生二粒小麦另一个抗条锈病基因 Yr1 5不同 ,研究证明 Yr1 5与 N or1图距是 1 1 .0 cm。
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| 关 键 词： | 小麦 野生二粒种群 抗条锈病基因 分子定位 |
Preliminary Molecular Mapping of Stripe rust Resistance Gene YrH52 Derived from Wild Emmer Wheat, Triticum dicoccoides |
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| Peng J. H. , Fahima T. Rder M. S. Li Y. C. , Dahan A. Grama A. Nevo E. Institute of Evolution,University of Haifa,Mount Carmel,Haifa ,Israel, Instiute for Plant Geneti.Preliminary Molecular Mapping of Stripe rust Resistance Gene YrH52 Derived from Wild Emmer Wheat, Triticum dicoccoides[J].Journal of Zhanjiang Ocean University,1999(4). |
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| Authors: | Peng J H Fahima T Rder M S Li Y C Dahan A Grama A Nevo E Institute of Evolution University of Haifa Mount Carmel Haifa Israel Instiute for Plant Geneti |
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| Institution: | Peng J. H. 1),* Fahima T. 1) Rder M. S. 2) Li Y. C. 1),* Dahan A. 1) Grama A. 3) Nevo E. 1) 1) Institute of Evolution,University of Haifa,Mount Carmel,Haifa 31905,Israel, 2) Instiute for Plant Geneti |
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| Abstract: | The main objective of present study is to molecularly map a stripe rust resistance gene derived from the unique Hermon population of wild emmer in Israel. An F 2 mapping population was established by crossing stripe rust resistant T. dicoccoides accession H52 from Mt. Hermon with T. durum cultivar Langdon. The stripe rust resistance of H52 was found to be controlled by a single dominant gene, which was provisionally designated as YrH52. Out of 120 microsatellite markers, 91% detected polymorphism between the parents. Among 79 segregating microsatellite loci generated from 56 microsatellite markers, nine were revealed to be linked to YrH52 with recombination frequency 0 02 to 0 35, genetic distance 2 00 to 43 37 cm, and LOD score 3 56 to 54 22. A genetic map of chromosome 1B consisting of ten microsatellite loci and YrH52. was constructed with a total map length of 101 5 cm. YrH52. is bracketed by Xgwm264a and Xgwm264c, and is closely linked to Xgwm264a, Xgwm18, then followed, in order, by Xgwm131a, Xgwm636b, Xgwm264c, Xgwm403a, Xgwm153, Xgwm550a, and Xgwm124. The YrH52. is also closely linked to RFLP marker Norl with 1 4 cm of map distance and 29 62 of LOD value, and may be different from another T. dicoccoides derived ssstripe rust resistance gene Yr15. which is 11 0 cm apart from Nor1 . |
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| Keywords: | Wheat Triticum dicoccoide Stripe rust resistance gene Molecular mapping |
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