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| 3-甲基胆蒽对斑马鱼组织病理效应的初步研究 | |
| 引用本文： | 杨蓉,聂芳红,孔庆波,谢英明,林红英,陈进军.3-甲基胆蒽对斑马鱼组织病理效应的初步研究[J].广东海洋大学学报,2008,28(4). |
| 作者姓名： | 杨蓉 聂芳红 孔庆波 谢英明 林红英 陈进军 |
| 作者单位： | 1. 广东海洋大学农业生物技术研究所,广东,湛江,524088 2. 广东海洋大学食品科技学院,广东,湛江,524088 3. 中国刑事警察学院警犬技术系,辽宁,沈阳,110034 |
| 基金项目： | 广东省自然科学基金，广东省国际科技合作计划 |
| 摘 要： | 分别用25、50、100、200和400μg/L的3-甲基胆蒽(3-MC)对斑马鱼进行水浴染毒,于第7天和第14天采集各染毒组和对照组活鱼的肝、鳃、心、肠和肾,进行病理组织学检查。结果发现,一定剂量的3-MC能导致肝细胞变性,胞浆内出现脂肪滴,严重时肝细胞坏死解体,界限模糊;心肌细胞萎缩坏死;肠粘膜上皮细胞纹状缘不整齐,有的脱落;鳃丝结构异常,鳃小片表面粗糙,上皮细胞不完整;肾小管上皮细胞肿胀,细胞界限不清,管腔内均质红染。供试斑马鱼的病理组织变化与3-MC的作用剂量和作用时间存在一定的关系。 |
| 关 键 词： | 3-甲基胆蒽 毒性 病理组织学 斑马鱼 |
Preliminary Studies on Histopathological Changes in Zebrafish Exposure to 3-Methylcholanthrene |
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| YANG Rong,NIE Fang-hong,KONG Qing-bo,XIE Ying-ming,LIN Hong-ying,CHEN Jin-jun.Preliminary Studies on Histopathological Changes in Zebrafish Exposure to 3-Methylcholanthrene[J].Journal of Zhanjiang Ocean University,2008,28(4). | |
| Authors: | YANG Rong NIE Fang-hong KONG Qing-bo XIE Ying-ming LIN Hong-ying CHEN Jin-jun |
| Abstract: | A range of zebrafish(Danio rerio) were exposed to 3-methylcholanthrene(3-MC) by water pollution at the doses of 25 μg/L,50 μg/L,100 μg/L,200 μg/L and 400 μg/L,respectively.On the 7th day and 14th day of exposure to 3-MC,the liver,gill,heart,intestine and kidney of the fish were collected to observe histopathological changes.It was found that the hepatocytes were denaturalized with fat drops in the cellular plasma and even showed necroses or disaggregation and obscure cellular borderlines,that there were atrophies and necroses in cardiac cells,that the villuses of the enteric epithelia were out of order and even fallen off,that the secondary lamella of the gill became coarse and some of the epithelia were lost,and that the epithelia of the renal tubules were swollen and the cellular borderlines were not clear,with even red dye in some tubules.It was also found that there were,to some extent,dose-effect and time-effect relationships between the histopathological changes and 3-MC. |
| Keywords: | 3-methylcholanthrene toxicity histopathology zebrafish(Danio rerio) |
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