| Effect of Bacillus baekryungensis YD13 Supplemented in Diets on Growth Performance and Immune Response of Sea Cucumber（Apostichopus japonicus） |
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| 作者姓名： | YAN Fajun TIAN Xiangli DONG Shuanglin |
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| 作者单位： | The Key Laboratory of Mariculture of Ministry of Education, Fisheries College, Ocean University of China,Qingdao 266003, P. R.China |
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| 基金项目： | supported by the National Programs for High Technology Research and Development of China （Grant No. 2006AA10Z409）; the program for Excellent Youth Foundation of Shandong Province （Grant No. JQ201009）; the program for New Century Excellent Talents in University （Grant No. NCET-08-0503） |
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| 摘 要： | The effect of a potential probiotic on the growth performance and immune response of sea cucumber（Apostichopus japonicus） was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses（0, the control; 1×10^4（YD134）, 1×10^6（YD136） and 1×10^8（YD138） CFU g-1 of diet）, and administered orally to A. japonicus（initial mean wet weight 5.44 g ± 0.17 g）. The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control（P 〈0.05）. Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×10^6 CFU g^-1（P 〈0.05）. The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×10^6 CFU g^-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.
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| 关 键 词： | 免疫应答 生长性能 芽孢杆菌 海参 刺参 超氧化物歧化酶 日粮 加味 |
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