| Histological Observation of Germ Cell Development and Discovery of Spermatophores in Ovoviviparous Black Rockfish（Sebastes schlegeli Hilgendorf） in Reproductive Season |
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| 作者姓名： | FENG Junrong ;LIU Liming ;JIANG Haibin ;WANG Maojian ;DU Rongbin |
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| 作者单位： | [1]Ocean College, Yantai University, Yantai 264005, P. R. China; [2]Shandong Marine Fisheries Research Institute, Yantai 264006, P. R. China |
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| 基金项目： | Acknowledgements We thank Dr. Daode Ji for providing microscope system. We also thank undergraduate students Jianwei Qiu and Haizhou Li for preparing samples. This study was supported financially by Grand Innovating Program of Agriculture Applying Technique in Shandong Province （No. 2008-109）, Modem Agricultural Industry Technology System in Shandong Province and the Science and Technology Development Program of Yantai （No. 2013 ZH088）. |
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| 摘 要： | Black rockfish（Sebastes schlegeli） is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season（October 2011–November 2012） with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.
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| 关 键 词： | 生殖细胞发育 组织学观察 许氏平鲉 石斑鱼 卵胎生 精荚 繁殖季节 精子细胞 |
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