| 鲤鱼（Cyprinuscarpio L．）体重和体长QTL的定位 |
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| 引用本文： | 刘继红,;张研,;常玉梅,;梁利群,;张晓峰,;鲁翠云,;徐美佳,;孙效文.鲤鱼（Cyprinuscarpio L．）体重和体长QTL的定位[J].湛江海洋大学学报,2009(4):19-25. |
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| 作者姓名： | 刘继红 ;张研 ;常玉梅 ;梁利群 ;张晓峰 ;鲁翠云 ;徐美佳 ;孙效文 |
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| 作者单位： | [1]黑龙江水产研究所北方鱼类生物工程育种重点开放实验室,黑龙江哈尔滨150070; [2]广西水产研究所,广西南宁530021; [3]中国水产科学研究院水产生物应用基因组研究中心,北京100141; [4]中山大学生命科学学院,广东广州510275 |
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| 基金项目： | 国家重点基础研究发展计划（2004CB117405）;农业部“引进国际先进农业科学技术”计划（2007-G55（A））. |
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| 摘 要： | 利用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记（共445个标记）对大头鲤／荷包红鲤抗寒品系的F2代雌核发育群体44个个体进行基因型检测，构建鲤鱼（CyprinuscarpioL.）遗传连锁图谱；利用软件WinQTLCart2.5采用复合区间作图法对体重和体长两个性状进行了QTL定位分析。结果共检测到与两个与体重性状相关的QTL，分别定位到LG24连锁群（qBWh-24-1）和LG20连锁群（qBWh-20-1）上，可解释的表型变异分别为7.98％和20.05％；检测到两个与体长性状相关的QTL，分别位于LG2连锁群（qFS-2-1）和LG20连锁群（qFS-20-1），可解释的表型变异分别为5.69％和12.69％，4个QTL的加性效应均为负值。
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| 关 键 词： | 鲤鱼 体长 体重 QTL |
Mapping QTLs Related to Body Weight and Full Size of Common Carp （Cyprinus carpio ） |
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| Institution: | LIU Ji-hong, ZHANG Yan, CHANG Yu-mei, LIANG Li-qun, LU Cui-yun, XU Mei-jia, SUN Xiao-wen(1. Heilongjiang Fisheries Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China; 2. Guangxi Institute of Fisheries, Nanning 530021, China; 3. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China; 4. College of Life Sciences, Sun-Yat Sen University, Guangzhou 510275, China) |
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| Abstract: | A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp× Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers （265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers）. Then quantitative traits loci （QTLs） associated with body weight, full size were identified by the composite interval mapping of the software Cartgrapher V.2.0. Two QTLs were identified for body weight on the linkage groups 24 and 20, which explained 7.98% and 20.05% of the total variation of the body weight, respectively. Both of the additive effects were negative. Two QTLs were identified for full size on the linkage groups 2 and 20, explaining 5.69% and 12.69% of the total variation of the full size, respectively. The additive effects were negative values. |
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| Keywords: | common carp （Cyprinus carpio） body weight full size quantitative trait loci |
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