| 基于cox1条形码的鱼肚DNA分子鉴定 |
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| 引用本文： | 朱惠敏,连一阳,王中铎,郭昱嵩,刘楚吾.基于cox1条形码的鱼肚DNA分子鉴定[J].湛江海洋大学学报,2014(6):1-5. |
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| 作者姓名： | 朱惠敏 连一阳 王中铎 郭昱嵩 刘楚吾 |
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| 作者单位： | 广东海洋大学水产学院、南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524025 |
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| 基金项目： | 国家自然科学基金青年基金（31201996）;广东省海洋渔业科技推广专项科技攻关与研发项目（A201101B02,A201208E01）;广东海洋大学大学生创新创业训练计划项目（1056613012） |
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| 摘 要： | 为鉴定鱼肚的正品来源,运用一对特异性引物扩增并测定10 个不同价格（700～1 500 元/kg）鱼肚样本的线粒体DNA 细胞色素c 氧化酶Ⅰ（cox1）约660 bp 的序列.通过BLASTN 比对DNA 序列同源度,判定鱼肚所属种类分别为鲈形目石首鱼科（Sciaenidae）、马鲅科（Eleutheronema）、笛鲷科（Lutjanidae）、带鱼科（Trichiuridae）.cox1 条码序列获取便捷,基于此条码序列的物种鉴定技术可用于鱼肚种类鉴别.
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| 关 键 词： | 鱼肚 DNA条形码 cox1 物种鉴定 |
DNA Molecular Identification of Fish Maw Based onMitochondrial cox1 Sequences |
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| ZHU Hui-min,LIAN Yi-yang,WANG Zhong-duo,GUO Yu-song,LIU Chu-wu.DNA Molecular Identification of Fish Maw Based onMitochondrial cox1 Sequences[J].Journal of Zhanjiang Ocean University,2014(6):1-5. |
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| Authors: | ZHU Hui-min LIAN Yi-yang WANG Zhong-duo GUO Yu-song LIU Chu-wu |
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| Institution: | (Key Laboratory of Aquaculture in South China Sea for Aquatic EconomicAnimal of Guangdong Higher Education Institutes // Fisheries College,Guangdong Ocean University, Zhanjiang 524025, China) |
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| Abstract: | In order to identify the counterfeit goods of fish maw, an accurate, simple method foridentification of fish species is needed. The part sequences of c oxidase I （cox1） gene about 660 basepairs were amplified and sequenced from 10 fish maw samples （the prices differ from 700 – 1 500 yuan/ kg） . Then the DNA sequences identified by BLASTN were category to Perciformes, and they wereclassified into 4 families： Sciaenidae, Eleutheronema, Lutjanidae, and Trichiuridae. The results showedthat cox1 barcoding sequences were obtained convenient, widely suitable for fish species identification. |
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| Keywords: | fish maw DNA barcoding cox1 species identification |
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