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| Purification and Refolding of a Novel β-Agarase from Inclusion Body of E. coli | |
| 作者姓名： | ZHANG Li LU Xinzhi HAN Feng MA Cuiping and YU Wengong* Marine Drug and Food Institute Ocean University of China Qingdao P.R. China |
| 作者单位： | ZHANG Li，LU Xinzhi，HAN Feng，MA Cuiping，and YU Wengong* Marine Drug and Food Institute，Ocean University of China，Qingdao 266003，P.R. China |
| 基金项目： | This work was supported by a grant from the National High Technology Research and Development Program of China (863 Program) (2004AA625020). |
| 摘 要： | 1 Introduction Agarases produced by agar-degrading bacteria are classified into two groups based on their modes of action, namely, α-agarase and β-agarase. They hydrolyze α -1, 3 linkage and β-1, 4 linkage of agarose respectively (Duckworth and Turvey, 1969). Agarases are widely used in food, cosmetic and medical industries for the produc-tion of oligosaccharides from agar (Kobayashi et al., 1997; Yoshizawa et al., 1995). Neoagaro-oligo- saccharides produced by β-agarase inhibit the gro… |
| 关 键 词： | β-agarase inclusion body refolding immobilized metal affinity chromatography |
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