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Response of Microbial Community to Petroleum Stress and Phosphate Dosage inSediments of Jiaozhou Bay,China
作者姓名:ZHAO Yangguo  CHEN Min  BAI Jie  LI Xinwei  Farhana Zulfiqar  and WANG Qianli
基金项目:supported by the National Natural Science Foundation of China (Grant No.40801193);the Basic Research Projects of Qingdao Science and Technology Program (12-1-4-1-(12)-jch);the Scientific Research Foundation for the Excellent Middle-Aged and Youth Scientists of Shandong Province of China (BS2011NJ018)
摘    要:


Response of microbial community to petroleum stress and phosphate dosage in sediments of Jiaozhou Bay,China
ZHAO Yangguo,CHEN Min,BAI Jie,LI Xinwei,Farhana Zulfiqar,and WANG Qianli.Response of Microbial Community to Petroleum Stress and Phosphate Dosage inSediments of Jiaozhou Bay,China[J].Journal of Ocean University of China,2014,13(2):249-256.
Authors:Yangguo Zhao  Min Chen  Jie Bai  Xinwei Li  Farhana Zulfiqar  Qianli Wang
Institution:1. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, P. R. China
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao, 266100, P. R. China
Abstract:The dynamic microcosms were used to evaluate the effect of oil spills on microbial ecological system in marine sediment and the enhancement of nutrient on the oil removal. The function and structure of microbial community caused by the oil pollution and phosphate dosage were simultaneously monitored by dehydrogenase activity assay and PCR-denaturing gradient gel electrophoresis (DGGE) techniques. The results indicated that the amount of total bacteria in all dynamic microcosms declined rapidly with incubation time. The number of petroleum-degrading bacteria and the activity of sediment dehydrogenase were gradually enhanced by petroleum in the oil-treated microcosms, while they both showed no obvious response to phosphate dosage. In comparison, phosphate spiked heterotrophic bacteria and they showed a significant increase in amount. DGGE profiles indicated that petroleum dosage greatly changed community structure, and the bacteria belonged to class Deltaproteobacteria, and phyla Bacteroidetes and Chlorobi were enriched. This study demonstrated that petroleum input greatly impacted the microbial community structure and consequently the marine sediment petroleum-degrading activity was enhanced. Phosphate dosage would multiply heterotrophic bacteria but not significantly enhance the petroleum degradation.
Keywords:marine sediments  oil spills  dehydrogenase activity  phosphate  heterotrophic bacteria  bacterial community
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