| Expression and Characterization of a Novel λ-Carrageenase Cgl150A＿Wa from Wenyingzhuangia aestuarii |
| |
| 作者姓名： | SUN Yuhao CAO Siqi ZHANG Yuying XUE Changhu XIAO Hang CHANG Yaoguang |
| |
| 作者单位： | 1. College of Food Science and Engineering,Ocean University of China;2. Marine Life Research Center,Laoshan National Laboratory |
| |
| 基金项目： | supported by the Fundamental Research Funds for the Central Universities (No. 202012020)；;the National Key R&D Program of China (No. 2018YFC 0311203)； |
| |
| 摘 要： | Carrageenan is a highly sulfated polysaccharide alternating of 1,4-O-α-D-galactopyranose-2,6-sulfate(D2S,6S) and 1,3-O-β-D-galactopyranose-2-sulfate(G2S). λ-Carrageenases are desirable tools for λ-carrageenan degradation. Based on the genome mining, a novel λ-carrageenase Cgl150A＿Wa was cloned from the bacterium Wenyingzhuangia aestuarii and expressed in Escherichia coli. Cgl150A＿Wa was an endo-acting enzyme and exhibited its maximum activity at 30℃ and pH 8.0. By employing a glycomics strategy ...
|
|
