A microsatellite genetic linkage map of black rockfish (Sebastes schlegeli) |
| |
| Authors: | Guannan Chu Liming Jiang Yan He Haiyang Yu Zhigang Wang Haibin Jiang Quanqi Zhang |
| |
| Institution: | 1. College of Marine Life Science, Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao, 266003, P. R. China
2. Shandong Marine Fisheries Research Institute, Yantai, 264006, P. R. China
|
| |
| Abstract: | Ovoviviparous black rockfish (Sebastes schlegeli) is an important marine fish species for aquaculture and fisheries in China. Genetic information of this species is scarce because of the lack of microsatellite markers. In this study, a large number of microsatellite markers of black rockfish were isolated by constructing microsatellite-enriched libraries. Female- and male-specific genetic linkage maps were constructed using 435 microsatellite markers genotyped in a full-sib family of the fish species. The female linkage map contained 140 microsatellite markers, in which 23 linkage groups had a total genetic length of 1334.1 cM and average inter-marker space of 13.3 cM. The male linkage map contained 156 microsatellite markers, in which 25 linkage groups had a total genetic length of 1359.6 cM and average inter-marker distance of 12.4 cM. The genome coverage of the female and male linkage maps was 68.6% and 69.3%, respectively. The female-to-male ratio of the recombination rate was approximately 1.07:1 in adjacent microsatellite markers. This paper presents the first genetic linkage map of microsatellites in black rockfish. The collection of polymorphic markers and sex-specific linkage maps of black rockfish could be useful for further investigations on parental assignment, population genetics, quantitative trait loci mapping, and marker-assisted selection in related breeding programs. |
| |
| Keywords: | black rockfish Sebastes schlegeli microsatellite genetic linkage map |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
|
