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Development of 57 novel polymorphic microsatellite markers in half-smooth tongue sole (Cynoglossus semilaevis)
Authors:Guidong Miao  Ying Xu  Di Wang  Songlin Chen  Tingjun Fan  Yongsheng Tian
Institution:1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China ;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China;Zibo Entry-Exit Inspection and Quarantine Bureau of P. R. China, Zibo 255031, P. R. China
2. Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China
3. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China
Abstract:Half-smooth tongue sole (Cynoglossus semilaevis) is a promising species for aquaculture in China.The wild population of C. semilaevis is under threat from environmental factors. Microsatellite markers are very suitable for assessing genetic diversity. Four microsatellite-enriched libraries of half smooth tongue sole (Cynoglossus semilaevis) were constructed,from which 57 polymorphic microsatellites were isolated and characterized.The polymorphism of these microsatellites was assessed by genotyping in 30 ind...
Keywords:Cynoglossus semilaevis  microsatellite  polymorphic marker  half-smooth tongue sole  
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