Identification of a putative tetrasporophyte-specific gene in <Emphasis Type="Italic">Gracilaria lemaneiformis</Emphasis> (Gracilariales,Rhodophyte) |
| |
| Authors: | Xueying Ren Xuecheng Zhang |
| |
| Institution: | (1) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, P. R. China |
| |
| Abstract: | A putative tetrasporophyte-specific gene, designated as SSH466 (GenBank accession No. DQ019223), was one of the genes identified
in this work using suppression subtractive hybridization (SSH) method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides,
including an open reading frame of 498 nucleotides encoding 166 amino acid residues, 158 nucleotides of 5′ untranslated region
and 363 nucleotides of 3′ non-coding region. Protein motif and secondary structure prediction showed that there existed a
transmembrane domain with a unique β-sheet. Thus, SSH466 protein might be a cross-membrane protein. Sequence homology search
in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed
that it was a tetrasporophyte-specific gene. |
| |
| Keywords: | suppression subtractive hybridization (SSH) tetrasporophyte female gametophyte Virtual Northern blot |
| 本文献已被 SpringerLink 等数据库收录！ |
|
