| Cloning and Characterization of an Abalone （Haliotis discus hannai ） Actin Gene |
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| 作者姓名： | MAHongming XUWei MAIKangsen LIUFUZhiguo CHENHong |
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| 作者单位： | MA Hongming，XU Wei，MAI Kangsen *，LIUFU Zhiguo，CHEN Hong The Key Laboratory of Mariculture of Chinese Ministry of Education，Ocean University of China，Qingdao 266003，P..R.China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，教育部海洋生物重点实验室基金 |
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| 摘 要： | 1 Introduction Actinisanimportantcontractileproteinineukary oticcells .Itisoneofthetwomajorcomponentsin volvedinthecontractionofmusclecells .Innon mus clecells ,itisthemajorpartofcytoskeletoninvolvedinmanyprocessessuchascellmotility ,endocytosis ,exocyt…
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| 关 键 词： | 鲍属动物 脱氧核糖核酸 无性繁殖 肌动蛋白 |
| 收稿时间： | 15 June 2004 |
| 修稿时间： | 16 August 2004 |
Cloning and characterization of an abalone (<Emphasis Type="Italic">Haliotis discus hannai</Emphasis>) actin gene |
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| MAHongming XUWei MAIKangsen LIUFUZhiguo CHENHong.Cloning and characterization of an abalone (Haliotis discus hannai) actin gene[J].Journal of Ocean University of China,2004,3(2):145-149. |
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| Authors: | Ma?Hongming Xu?Wei Email author" target="_blank">Mai?Kangsen?Email author Liufu?Zhiguo Chen?Hong |
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| Institution: | The Key Laboratory of Mariculture of Chinese Ministry of Education, Ocean University of China, Qingdao 266003, P.R.China |
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| Abstract: | An actin encoding gene was cloned by using RT-PCR, 3′ RACE and 5′ RACE from abalone Haliotis discus hannai. The full length of the gene is 1532 base pairs, which contains a long 3′ untranslated region of 307 base pairs and 79 base
pairs of 5′ untranslated sequence. The open reading frame encodes 376 amino acid residues. Sequence comparison with those
of human and other mollusks showed high conservation among species at amino acid level. The identities was 96%, 97% and 96%
respectively compared with Aplysia californica, Biomphalaria glabrata and Homo sapience β-actin. It is also indicated that this actin is more similar to the human cytoplasmic actin (β-actin) than to human muscle
actin. |
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| Keywords: | Haliotis discus hannai actin cDNA cloning |
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