Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179 |
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| Authors: | Kehou Pan Xiaolei Ma Jianzhong Yu Baohua Zhu Guanpin Yang |
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| Institution: | 1. Laboratory of Applied Microalgae Biology, the Key Laboratory of Aquaculture, Ministry of Education,Ocean University of China, Qingdao 266003, P R. China
2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P R. China |
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| Abstract: | Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase
play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity
to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana. |
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| Keywords: | Nannochloropsis oculata gene cloning elongase PUFAs |
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