| The Complete Sequence of Mitochondrial COⅡ Gene of Fenneropenaeus chinensis and Its Applicability as a Marker for Phylogenetic Analysis |
| |
| 作者姓名： | YU Shanshan KONG Xiaoyu LI Yulong XU Hui |
| |
| 作者单位： | Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China |
| |
| 基金项目： | This research was supported by the 863 High Technology Project （2002AA626062）. |
| |
| 摘 要： | The complete mitochondrial cytochrome oxidase subunit Ⅱ （COⅡ） gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A＋T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level.
|
| 关 键 词： | 中国对虾 线粒体COⅡ基因 完整序列 系统发生分析 标记物 |
| 收稿时间： | 2006-03-06 |
| 修稿时间： | 2006-11-15 |
The complete sequence of mitochondrial COII gene of <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> and its applicability as a marker for phylogenetic analysis |
| |
| YU Shanshan KONG Xiaoyu LI Yulong XU Hui.The complete sequence of mitochondrial COII gene of Fenneropenaeus chinensis and its applicability as a marker for phylogenetic analysis[J].Journal of Ocean University of China,2007,6(2):187-192. |
| |
| Authors: | Yu Shanshan Kong Xiaoyu Li Yulong Xu Hui |
| |
| Institution: | (1) Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, 266003, P. R. China |
| |
| Abstract: | The complete mitochondrial cytochrome oxidase subunit II (COII) gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence
similarity to Marsupenaeus japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A+T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively.
The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those
estimated with COI and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COII gene has a faster evolutionary
rate than that of the COI gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level.
The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the
four subgenera to genus level. |
| |
| Keywords: | Fenneropenaeus chinensis COII gene phylogenetic analysis |
| 本文献已被 维普 SpringerLink 等数据库收录！ |
