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Variation of Bioaccumulation Ability of 2,2’,4,4’- Tetrabromodiphenyl Ether by Marine Diatom Skeletonema costatum Under Different N:P Ratios
作者姓名:CHAI Chao  GE Wei  YIN Xundong
作者单位:[1]College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, P. R. China [2]College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P. R. China
基金项目:This work was supported by the National Natural Science Foundation of China (No. 40906061), the Science and Technology Plan Projects of Qingdao (No. 12-1-3- 64-nsh), the 'Two Districts' Foundation of Shandong Province, China (No. 2011-Yellow-19) and the Talent Foundation of Qingdao Agricultural University (No. 630642).
摘    要:The growth, biochemical content and bioaccumulation quantity of 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) in Skeletonema costatum were studied under different N:P ratios (1, 4, 16, 64 and 128). All cellular biochemical contents of S. costatum presented decreasing trend over cultivation time. At early stage of cultivation, the cellular protein, carbohydrate and lipid in S. co-statum presented higher values in treatments of N:P=4 and 16. However, they were lower in these treatments at the late stage, but higher in treatments N:P=1 and 128. Similarly, BDE-47 levels per cell of S. costatum were higher in treatments of N:P=4 and 16 at early stage of cultivation, which were 3.8 and 3.7 ng (106 cells)-1, respectively. At the middle stage of cultivation, the BDE-47 level per S. costatum cell lowered;and it further reduced in the treatments of N:P=4 and 16 at the late stage with the values 0.6 and 0.5 ng (106 cells)-1, respectively. However, it rose in N:P=128, reaching up to 2.3 ng (106 cells)-1. Compared with BDE-47 per cell, BDE-47 per algal volume under different N:P ratios did not present obvious difference. The quantity BDE-47 accumulated per cell of S. co-statum was positively correlated with protein, carbohydrate and lipid per cell; meanwhile, the BDE-47 per volume had a positive correlation with biochemical content per volume. The variation of bioaccumulation ability of BDE-47 in S. costatum can be ex-plained by biochemical changes due to N:P ratios.

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