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| Determination of Selenium in Marine Aquatic Products by Hydride Generation-atomic Fluorescence Spectrometry （HG-AFS） | |
| 作者姓名： | ZHANG Zhaohui GAO Xin Tashiro Yuri Hiroo Ogawa |
| 作者单位： | [1]Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan [2]Department of Food Engineering, Ocean University of China, Qingdao 266003, P.R. China |
| 摘 要： | A method for the analysis of selenium in marine aquatic products by HG-AFS has been investigated. The method is based on the reduction of inorganic selenium to volatile SeH2 which is bubbled out by carrier gas of pure argon, and then swept to Ar-H2 flame quarts atomizer to measure its fluorescence intensity. The hydride generation, transportation, atomization and some instrumental parameters were studied by a kind of orthogonal design. The optimum conditions selected are as follows： reactive acidity, 20% HC1; the amount of NaBH4, 4.9mL; gas flow of argon, 600mLmin^-1; atomizing temperature, 200 ℃ ; negative high voltage, - 300V; light current, 100 mA; integral time, 7s. The detection limit of the presented method is 0.072μgL^-1 for selenium. The calibration curve shows a satisfactory line inthe concentration range from 0.000 to 1.000μgL^-1 Se. The recovery is 95.8%-102.2%. |
| 关 键 词： | 硒 水产食品 氢化物 荧光性 光谱测定法 |
| 文章编号： | 1672-5182（2005）03-252-05 |
| 收稿时间： | 2005-02-17 |
| 修稿时间： | 2005-06-02 |
Determination of selenium in marine aquatic products by hydride generation-atomic fluorescence spectrometry (HG-AFS) |
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| ZHANG Zhaohui GAO Xin Tashiro Yuri Hiroo Ogawa.Determination of selenium in marine aquatic products by hydride generation-atomic fluorescence spectrometry (HG-AFS)[J].Journal of Ocean University of China,2005,4(3):252-256. | |
| Authors: | Zhaohui Zhang Xin Gao Tashiro Yuri Hiroo Ogawa |
| Institution: | 1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan;Department of Food Engineering, Ocean University of China, Qingdao 266003, P.R. China 2. Department of Food Engineering, Ocean University of China, Qingdao 266003, P.R. China 3. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan |
| Abstract: | A method for the analysis of selenium in marine aquatic products by HG-AFS has been investigated. The method is based on the reduction of inorganic selenium to volatile SeH2 which is bubbled out by carrier gas of pure argon, and then swept to Ar-H2 flame quarts atomizer to measure its fluorescence intensity. The hydride generation, transportation, atomization and some instrumental parameters were studied by a kind of orthogonal design. The optimum conditions selected are as follows: reactive acidity, 20% HC1; the amount of NaBH4, 4.9mL; gas flow of argon, 6OO mLmin−1; atomizing temperature, 200°C, negative high voltage,−300V; light current, 100 mA; integral time, 7 s. The detection limit of the presented method is 0.072μgL−1 for selenium. The calibration curve shows a satisfactory line in the concentration range from 0.000 to l.OOOμgL−1 Se. The recovery is 95.8%–102.2% . |
| Keywords: | hydride generation-atomic fluorescence spectrometry (HG-AFS) selenium marine aquatic products |
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