| Production of the Blood Pressure Lowing Peptides from Brown Alga （ Undaria pinnatifida ） |
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| 作者姓名： | Sato Minoru Oba Takashi Hosokawa Takao Yamaguchi Toshiyasu Nakano Toshiki Saito Tadao Muramoto Koji Kahara T akashi Funayama Katsura Kobayashi Akio Nakano Takahisa |
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| 作者单位： | [1]Graduate School of Agricultural Science, Tohoku University, Aoba-Ku, Sendai, Miyagi, 981-8555, Japan [2]Riken Vitamin Co., Ltd., 2-9-18 Misaki-Cho, Chiyoda-Ku, Tokyo, 101-8370, Japan |
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| 摘 要： | Brown alga （ Undaria pinnatifida） was treated with alginate lyase and hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates for the angiotensin-I-converting enzyme （ACE） was measured. Four hydrolysates with potent ACE-inhibitory activity were administered singly and orally to spontaneously hypertensive rats （SHRs）. The systolic blood pressure of SHRs decreases significantly after single oral administration of the brown alga hydrolysates by pro- tease S ＇ Amano＇ （from Bacillus stearothermophilus） at the concentration of 10 （mg protein） （kg body weight）^ - 1. In the 17 weeks of feeding experiment, 7-week-old SHRs were fed standard diet supplemented with the brown alga hydrolysates for 10 weeks. In SHRs fed 1.0 and 0.1% brown alga hydrolysates, elevating of systolic bloodpressure was significantly suppressed for 7 weeks. To elucidate the active components, the brown alga hydrolysates were fractionated by 1-butanol extraction and HPLC on a reverse-phase column. Seven kinds of ACE-inhibitory peptides were isolated and identified by amino acid composition analysis, sequence analysis, and LC-MS with the results Val-Tyr, Ile-Tyr, Ala-Trp, Phe-Tyr, Val-Trp, Ile-Trp, and Leu-Trp. Each peptide was determined to have an antihypertensive effect after a single oral administration in SHRs. The brown alga hydrolysates were also confirmed to decrease the blood pressure in humans.
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| 关 键 词： | 海藻 缩氨酸 抗血压药物 藻酸盐 水解工艺 |
| 文章编号： | 1672-5182（2005）03-209-05 |
| 收稿时间： | 2005-01-10 |
| 修稿时间： | 2005-06-23 |
Production of the blood pressure lowing peptides from brown alga (Undaria pinnatifida) |
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| Sato Minoru Oba Takashi Hosokawa Takao Yamaguchi Toshiyasu Nakano Toshiki Saito Tadao Muramoto Koji Kahara T akashi Funayama Katsura Kobayashi Akio Nakano Takahisa.Production of the blood pressure lowing peptides from brown alga (Undaria pinnatifida)[J].Journal of Ocean University of China,2005,4(3):209-213. |
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| Authors: | Sato Minoru Oba Takashi Hosokawa Takao Yamaguchi Toshiyasu Nakano Toshiki Saito Tadao Muramoto Koji Kahara Takashi Funayama Katsura Kobayashi Akio Nakano Takahisa |
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| Institution: | (1) Graduate School of Agricultural Science, Tohoku University, Aoba-Ku, 981-8555 Sendai, Miyagi, Japan;(2) Riken Vitamin Co., Ltd., 2-9-18 Misaki-Cho, Chiyoda-Ku, 101-8370 Tokyo, Japan |
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| Abstract: | Brown alga (Undaria pinnatifida) was treated with alginate lyase and hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates
for the angiotensin-I-converting enzyme (ACE) was measured. Four hydrolysates with potent ACE-inhibitory activity were administered
singly and orally to spontaneously hypertensive rats (SHRs). The systolic blood pressure of SHRs decreases significantly after
single oral administration of the brown alga hydrolysates by protease S ‘Amano’ (from Bacillus stearothermophilus) at the concentration of 10 (mg protein) (kg body weight)−1. In the 17 weeks of feeding experiment, 7-week-old SHRs were fed standard diet supplemented with the brown alga hydrolysates
for 10 weeks. In SHRs fed 1.0 and 0.1% brown alga hydrolysates, elevating of systolic bloodpressure was significantly suppressed
for 7 weeks. To elucidate the active components, the brown alga hydrolysates were fractionated by 1-butanol extraction and
HPLC on a reverse-phase column. Seven kinds of ACE-inhibitory peptides were isolated and identified by amino acid composition
analysis, sequence analysis, and LC-MS with the results Val-Tyr, Ile-Tyr, Ala-Trp, Phe-Tyr, Val-Trp, Ile-Trp, and Leu-Trp.
Each peptide was determined to have an antihypertensive effect after a single oral administration in SHRs. The brown alga
hydrolysates were also confirmed to decrease the blood pressure in humans. |
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| Keywords: | brown alga blood pressure lowing antihypertensive peptide |
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