Sequences Characterization of Microsatellite DNA Sequences in Pacific Abalone (Haliotis discus hannat) |
| |
| Authors: | LI Qi Kijima Akihiro |
| |
| Institution: | 1. The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China
2. Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan |
| |
| Abstract: | The microsatellite-enriched library was constructed using magnetic bead hybridization selection method, and the microsatellite DNA sequences were analyzed in Pacific abalone Haliotis discus hannai. Three hundred and fifty white colonies were screened using PCR-based technique, and 84 clones were identified to potentially contain microsatellite repeat motif. The 84 clones were sequenced, and 42 microsatellites and 4 minisatellites with a minimum of five repeats were found (13.1% of white colonies screened). Besides the motif of CA contained in the oligoprobe, we also found other 16 types of microsatellite repeats including a dinucleotide repeat, two tetranucleotide repeats, twelve pentanucleotide repeats and a hexanucleotide repeat. According to Weber(1990), the microsatellite sequences obtained could be categorized structurally into perfect repeats (73.3%), imperfect repeats(13.3%), and compound repeats (13.4%). Among the microsatellite repeats, relatively short arrays (＜ 20 repeats) were most abundant,accounting for 75.0%. The largest length of microsatellites was 48 repeats, and the average number of repeats was 13.4. The data on the composition and length distribution of microsatellites obtained in the present study can be useful for choosing the repeat motifs for microsatetlite isolation in other abalone species. |
| |
| Keywords: | Haliotis discus hannai microsatellite DNA sequence characterization |
| 本文献已被 万方数据 等数据库收录！ |
|
