| 引用本文: | 纪凯,黄天晴,谷伟,刘恩慧,王高超,王炳谦,郭福元,曹学彬,郑龙华,董福霖,徐革锋.温度和盐度对虹鳟(Oncorhynchus mykiss)“水科1号”生长、消化酶活性和相关基因表达的影响.海洋与湖沼,2023,54(5):1476-1487. |
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| 温度和盐度对虹鳟(Oncorhynchus mykiss)“水科1号”生长、消化酶活性和相关基因表达的影响 |
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纪凯1,2, 黄天晴1, 谷伟1, 刘恩慧1, 王高超1, 王炳谦1, 郭福元3, 曹学彬3, 郑龙华3, 董福霖3, 徐革锋1
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1.农业农村部淡水水产生物技术与遗传育种重点实验室 中国水产科学研究院黑龙江水产研究所 黑龙江哈尔滨 150070;2.上海海洋大学水产与生命学院 上海 201306;3.烟台经海海洋渔业有限公司 山东烟台 264006
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| 摘要: |
| 温度和盐度变化的影响是虹鳟(Oncorhynchus mykiss)海水驯化和养殖生产中需要解决的重要问题。为探究温度和盐度对虹鳟生长发育的影响, 通过在3种温度(10、16和22 °C)条件下均设置5种盐度梯度处理(盐度分别为0、8、16、24、32), 分别以A1~A5表示10 °C各盐度组, 以B1~B5表示16 °C各盐度组, 以C1~C5表示22 °C各盐度组, 将温度16 °C且盐度为0组(B1)作为淡水对照组, 开展为期21 d的养殖实验。养殖实验结束后分析不同温度和盐度对虹鳟生长、肠道消化酶活性、鳃和肠组织中生长相关基因(gh和igf-1)、应激相关基因(hsp70)和渗透压调节相关基因(aqp3和nka)表达的影响。结果显示,除B2 (16 °C下盐度为8)盐度组的饵料转化率(FCE)、增重率(WGR)和特定生长率(SGR)较B1 (淡水对照组)显著升高, 肠道中淀粉酶和脂肪酶活性分别较B1 (淡水对照组)显著升高约23%和8%外(P＜0.05), 其余实验组以上指标均较淡水对照组降低。此外, 鳃和肠组织中, B2和B3组gh和igf-1的表达量较对照组显著上调(P＜0.05), 而其余实验组gh和igf-1的表达量较对照组均显著下调(P＜0.05)。鳃组织中B2和B3组以及肠组织中B2组的hsp70表达量较对照组无显著差异(P＞0.05), 其余实验组hsp70的表达量较对照组显著上调(P＜0.05)。不同温度条件下, 鳃组织中aqp3表达量随实验组盐度增大呈下调趋势, 而肠组织中则呈上调趋势;两组织中nka表达量随实验组盐度增大均呈上调趋势。研究结果表明, 16 °C下8盐度组的虹鳟较淡水组生长更好。因此, 在虹鳟适宜的温度下,适当地提升养殖水体的盐度有利于促进虹鳟的生长发育, 这为完善海水条件下虹鳟的驯化和养殖提供了基础资料。 |
| 关键词: 虹鳟 温度 盐度 生长 消化酶活性 基因表达 |
| DOI：10.11693/hyhz20221200345 |
| 分类号:S917.4;Q591 |
| 基金项目:财政部和农业农村部:国家现代农业产业技术体系资助,CARS-46号;中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助,2020TD32号;烟台开发区创新创业团队项目,2021TD003号。 |
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| EFFECTS OF TEMPERATURE AND SALINITY ON GROWTH, INTESTINAL DIGESTIVE ENZYME ACTIVITY, AND GENE EXPRESSION IN TISSUES OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) |
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JI Kai1,2, HUANG Tian-Qing1, GU Wei1, LIU En-Hui1, WANG Gao-Chao1, WANG Bing-Qian1, GUO Fu-Yuan3, CAO Xue-Bin3, ZHENG Long-Hua3, DONG Fu-Lin3, XU Ge-Feng1
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1.Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2.College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China;3.Yantai Jinghai Marine Fishery Co. Ltd., Yantai 264006, China
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| Abstract: |
| Temperature and salinity are key factors on the growth and development of rainbow trout Oncorhynchus mykiss. To understand the effects of temperature, salinity, and their interaction, we conducted a 21-day experimental study in which combinations of temperatures (10, 16 and 22 °C) and salinities (0, 8, 16, 24, 32) were tested. The salinity groups at 10 °C are denoted as A1~A5 (1~5 represent the five salinity levels, respectively), 16 °C as B1~B5, and 22 °C as C1~C5. The 16 °C and 0 salinity group (B1) was used as the freshwater control group. At the end of the experiments, effects of different temperatures and salinities on rainbow trout growth, intestinal digestive enzyme activity, expression of growth-related genes (growth hormone, gh and insulin-like growth factor-1, igf-1), stress-related genes (heat shock protein, hsp70), and osmolarity-regulated genes (Aquaporin3, aqp3 and Na+/K+-ATPase, nka) were recorded and analyzed statistically. Results show that the feed conversion rate (FCE), weight gain rate (WGR), and specific growth rate (SGR) were significantly higher in B2 (16 °C, salinity 8) group compared to B1 (freshwater control). Amylase and lipase activities in the intestine were significantly higher in B2 group than B1’s by 23% and 8%, respectively (P＜0.05). The other groups showed a decrease in the abovementioned indices compared to the control group. In addition, the expressions of gh and igf-1 in gill and intestinal tissues were significantly up-regulated (P＜0.05) in the B2 and B3 groups. In contrast, compared to the control, the expressions of gh and igf-1 in the rest of the groups were significantly down-regulated (P＜0.05); those of hsp70 in B2 and B3 groups in gill tissues and B2 group in intestinal tissues showed no significant difference (P＞0.05); and those of hsp70 in the other groups was significantly up-regulated (P＜0.05). Under different temperature conditions, the expression of aqp3 in gill tissues was down-regulated with increasing salinity, while it was up-regulated in intestinal tissues; the expression of nka in both tissues was up-regulated with increasing salinity. Rainbow trout of B2 group (16 °C, salinity 8) grew the best. Therefore, under a suitable temperature, a certain increase of salinity shall be beneficial to the growth and development of rainbow trout. This study provided basic information for improvement in the domestication and culture of rainbow trout in seawater. |
| Key words: rainbow trout (Oncorhynchus mykiss) temperature salinity growth digestive enzyme activity gene expression |
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