| 西施舌3 个自然群体杂合性的研究 |
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| 引用本文： | 王展林,黎中宝,张桂玲,陈锦,赵斌丽,吴宁,林小云.西施舌3 个自然群体杂合性的研究[J].海洋科学,2009,33(7):70-74. |
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| 作者姓名： | 王展林 黎中宝 张桂玲 陈锦 赵斌丽 吴宁 林小云 |
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| 作者单位： | 集美大学,水产学院,集美大学水产生物技术研究所,福建省高校水产科学技术与食品安全重点实验室,福建,厦门,361021 |
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| 基金项目： | 福建省自然科学基金，福建省高等教育新世纪优秀人才支持计划项目，福建省高校水产科学技术与食品安全重点实验室基金，集美大学创新团队基金 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳技术,对广西北海、福建漳港和江苏启东3个西施舌(Coelomactra antiquata (Spengler))自然群体杂合性进行了研究.共检测分析了7个酶,获得12个位点,25个等位基因.其中4个位点是多态的,分别是Sod-1、Mdh-1、Est-1、Est-3,这些位点分别有1～7个等位基因.结果表明,福建漳港群体的Sod-1和Est-1位点、广西北海群体的勋Sod-1位点符合Hardy-weinberg平衡(P>0.05);江苏启东群体的多态位点中所有位点、福建漳港群体的Mdh-1、Est-3位点,广西北海群体的Mdh-1、Est-1、Est-3位点均非常显著偏离Hardy-weinberg平衡(P<0.01).另外,除了江苏启东和福建漳港群体的Est-1位点表现出杂合子缺失(F>0)外,各群体中每个位点均表现出杂合子过量(F<0).结合各群体中每个多态位点的观察杂合度和期望杂合度,认为中国东南沿海西施舌的种质资源状况良好.
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| 关 键 词： | 西施舌(Coelomactra antiquata(spengler)) 等位酶 群体 杂合性 |
| 收稿时间： | 2008/10/20 0:00:00 |
| 修稿时间： | 1/5/2009 12:00:00 AM |
Heterozygosity in 3 natural stocks of Coelmomactra antiquata (Spengler) |
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| WANG Zhan-lin,LI Zhong-bao,ZHANG Gui-ling,CHEN Jin,ZHAO Bin-li,WU Ning and LIN Xiao-yun.Heterozygosity in 3 natural stocks of Coelmomactra antiquata (Spengler)[J].Marine Sciences,2009,33(7):70-74. |
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| Authors: | WANG Zhan-lin LI Zhong-bao ZHANG Gui-ling CHEN Jin ZHAO Bin-li WU Ning and LIN Xiao-yun |
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| Abstract: | Heterozygosity was investigated using an assay of vertical slab polyacrylamide gel electrophoresis in three Coelmomactra antiquate stocks from three sites: Beihai (Guangxi Province), Zhanggang (Fujian Province), Qidong (Jiangsu Province). 7 allozymes were detected, and got 12 allozyme loci, 25 alleles, 4 loci (Sod-1,Mdh-1,Est-1,Est-3) with 1-7 alleles which are polymorphic. The results showed that the genetypic distribution observed at Sod-1, Est-1 in Zhanggang stock and Sod-1 in Beihai stock were corresponded with the Hardy-Wemberg equilibrium (P>0.05). But other loci in the three stocks were significant deviations from Hardy-Weinberg equilibrium (P<0.01). There was heterozygote deficiency existed in Est-1 in Qidong and Zhanggang stock (F>0), and heterozygote excess existed in other loci in the three stocks (F<0). The observed heterozygosity and expected heterozygosity per polymorphic locus per stock indicated that the germplasm resources of three C. antiquate stocks were good. |
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| Keywords: | Coelmomactra antiquata (Spengler) allozyme natural stock heterozygosity |
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