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1.
Influence of IHSS Standard and Reference Materials on Copper and Mercury Toxicity to Vibrio fischeri
The reduction in light emission of the marine bacterium Vibrio fischeri used in the standard Microtox® bioassay was measured for the metals copper and mercury. The concentration at which the light emission was reduced by 50% (EC50) was determined to be (3.43 ± 0.83) μmol/L for Cu2+ and (0.66 ± 0.01) μmol/L for Hg2+. The reduction of the toxicity of these metals by humic and fulvic acids were studied using IHSS Standard and Reference Materials. Copper toxicity was reduced 17...20% by the soil and peat fulvic acids and 9...20% by the aquatic fulvic acids. While there appeared to be little difference in the reduction of Cu toxicity by fulvic acids from soils, peats, or aquatic systems, Hg toxicity was reduced 3.6...7.3% by the soils and peats, while aquatic fulvic acids reduced Hg toxicity 14...16%. Soil fulvic acids appear to have significantly less capacity to reduce Hg toxicity than Cu toxicity. Humic acids had much higher reductions of Cu toxicity (44...124%) compared to the fulvic acids, with little difference between aquatic and soil or peat humic acids, 44...124% and 67...100%, respectively. However, humic and fulvic acids, regardless of origin, had approximately the same effect on Hg toxicity with 3.5...16% reduction by fulvic acids and 8...20% reduction by humic acids. Unlike the fulvic acids, no clear trend was observed relative to origin of the humic acids. There was no correlation between percent reduction of Cu or Hg toxicity by the organic compounds and copper binding capacity (CuBC), C/N ratio, or carboxyl content of the materials. Examination of natural organic matter (NOM) isolated by reverse osmosis techniques from three water sources had reductions of both Cu and Hg toxicity that were most similar to the Suwannee River and Nordic fulvic acids. 相似文献
2.
The effect of land reclamation on the migration and accumulation of heavy metals and some pollutants in small rivers is determined. The study was conducted in the Middle Amur lowland in different phases of hydrological regime and at different extent of floodplain inundation. The formation of the hydrological regime in small rivers in this area is governed by the irregular annual runoff distribution. The seasonal character of flood periods requires water samples to be taken before spring flood (April) and after floods have passed (September–October), and at various extent of floodplain inundation. The field studies of water-courses were carried out in 2009–2014. The water samples were analyzed to determine the concentrations of heavy metals (iron, manganese, copper, nickel, cobalt, lead and zinc), suspended substances, organic carbon (total, dissolved, and suspended), humic and fulvic acids, and volatile organic compounds. The mobility of heavy metals in surface watercourses was shown to reflect the effect of drainage and surface runoff from soil horizons, an increase in the concentrations of suspended and organic compounds, especially, fulvic acids, which enhance their geochemical mobility. During floods, an increase in heavy metals washout from floodplain soils and the dilution of their concentrations causes the formation of a single-type concentration series of heavy metals. In addition, the processes of pollutants migration show an effect of changes in the geomorphological characteristics of floodplain–channel complexes and a decrease in flow velocity in watercourses in the areas where land reclamation was applied. Thus, all factors mentioned above lead to a decrease in water pollution index in the river. 相似文献
3.
John D. Coates Romy Chakraborty Susan M. O&#x;Connor Claudia Schmidt Jürgen Thieme 《洁净——土壤、空气、水》2001,28(7):420-427
As part of a study on microbial redox alteration of humic substances we investigated the potential effect of this metabolism on the fate of heavy metals and hydrocarbons as a result of conformational alteration of the humic molecular structure due to microbial reduction. Our studies indicate that the microbial reduction of humic acids (HA) results in significant morphological and geochemical alterations. X‐ray microscopy analysis indicate that the conformational structure of the humic colloids is altered as a result of the redox change. In the reduced state, the HA appeared as small dense particles, on reoxidation, large loose aggregates were formed. In addition, spectrofluorometric studies indicated that the binding capacity of the HA for naphthalene was decreased by 10% when the HA was reduced. Similarly, the reduced HA yielded higher surface tension values at all concentrations tested which is indicative of a more hydrophilic and less hydrophobic solute. On reoxidation, the surface tension values reverted back to values similar to those obtained for the untreated oxidized HA. These data indicate that the hydrophobicity of the HA is altered on biological reduction of the HA and that this alteration is reversible. In contrast the reduced HA demonstrated a 15% higher affinity for heavy metals such as divalent cobalt than the oxidized HA. In addition to increasing the binding capacity of HA for heavy metals, the reduction of the HA also decreased the bioavailability and toxicity of bound heavy metals such as chromium. When incubated in the presence of Cr(III) and HA, cells of Escherichia coli grew much more rapidly in the presence of the reduced HA suggesting that the higher metal binding capacity of the reduced humic substances resulted in a removal of the Cr(III) from solution and hence reduced its bioavailability and toxicity. These studies demonstrate that HA redox state and reduction of humic acids by microorganisms can have a significant effect on the molecular morphology and binding constants of HA for heavy metals and hydrocarbons and also directly affects the bioavailability of these compounds in the environment. 相似文献
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Sixteen samples of fulvic acids and XAD‐4 fractions of riverine, estuarine, coastal, and open ocean origin have been studied by emission and synchronous molecular fluorescence spectroscopy. Certain features of the molecular fluorescence are related to the nature, the content, and the origin of those aquatic humic substances (HS). Riverine HS appear several times richer in fluorophores than marine HS, which can be well observed by emission fluorescence spectroscopy. Synchronous‐scan spectra of fulvic acids and of XAD‐4 fractions from the aquatic environments studied, emphasized the quality differences of their fluorophores. These features are useful as tracers of humic substances related with their natural environment source or even with their anthropogenic origin. 相似文献
6.
M. P. Smirnov 《Water Resources》2008,35(4):460-468
Carbon concentrations and ratios in humic and fulvic acids and their shares in the total Corg in river waters of the CIS is studied with the use of the results of four-year observations in 60 typical points in several latitudinal zones and altitudinal zones of different types in connection with landscape conditions. 相似文献
7.
H.-J. Walther 《洁净——土壤、空气、水》1985,13(2):223-228
For the purpose of utilizing domestic flocculants for the treatment of water and of reducing by this the equivalent imports of metal salts, in this paper the result of investigations made on model waters and on a river water sample is described. The model waters contained the pesticides Atrazin, Diuron and 2,4-D, which partly were polluted in addition with different quantities of fulvic and humic acids as well as with colloidal substances causing turbidity (Friedland clay and finely dispersed cellulose). The investigations were supplemented by testing several adsorption media as γ-aluminium oxide, active carbon and diatom earth. In the presence of fulvic and humic substances one does not succeed in eliminating the investigated herbicides by flocculation with lime hydrate. Only after chemical side reactions between these contained substances the adsorbability is clearly influenced. The different elimination efficiencies at different reaction conditions are explained in detail. 相似文献
8.
A method is described for the specific hydrolysis of amino acids from humic substances by using the proteolytic enzyme “Pronase E”. The amino acids are analyzed by a fluorescence detector after precolumn derivatization and HPLC-separation. The pattern and the concentration of amino acids after the enzymatic hydrolysis are set up for humic substances of different origin. Due to the results the enzymatic hydrolysis is suitable for the characterization of humic substances with a nitrogen content higher than 3%. As shown in a kinetic study of the hydrolysis of a fulvic acid the influence of humic substances on the enzyme activity increases in case of lower nitrogen content. Due to the specificity of the proteolytic enzyme the operationally defined method is limited as shown by a protein of a known amino acid content. The amino acids leucine, valine, tyrosine and histidine are released to the highest extent. The determined total extent for the hydrolysis of Insulin B is 26.34% and for Casein 38.37%. By comparison of the enzymatic hydrolysis with the classic acid hydrolysis the advantages of the biochemical method are the simple experimental handling and the mild reaction conditions. Amino acids which are very unstable like glutamine and asparagine can only be analyzed by the enzymatic hydrolysis. 相似文献
9.
洱海沉积物有色可溶性有机物(CDOM)三维荧光空间分布特性及指示意义 总被引：1，自引：0，他引：1
采用荧光光谱区域体积积分法定量研究洱海沉积物有色可溶性有机物（CDOM）三维荧光空间分布特性,结果表明,洱海沉积物CDOM中类腐殖酸组分占比最高（44.5%~74.2%）,其次为溶解性微生物代谢产物（10.7%~20.4%）和类富里酸物质（8.19%~19.7%）,而类蛋白组分占比最低,类腐殖酸占比与H/C和N/C均呈显著负相关;南部湖心平台区域沉积物类富里酸和类蛋白组分占比较高,其CDOM自生源占比较高;北部水生植物分布区溶解性微生物代谢产物占比较高,CDOM陆源性较强;中部深水区及南北湖湾区域类腐殖酸占比较高,随水深增加沉积物CDOM自生源特征增强.随沉积物深度增加类腐殖酸和溶解性微生物代谢产物占比呈下降趋势,类富里酸占比呈上升趋势,CDOM自生源特征增强,脂肪化程度增高.随湖泊富营养化程度增加沉积物CDOM受微生物代谢产物影响增强,陆源性增加,而自生源性降低,湖泊沉积物CDOM具有富营养化指示意义. 相似文献
10.
Humic acid plays an important role in the distribution of heavy metals in the environment. The aims of this study were conducted to evaluate the spectroscopic characteristics and aluminum (Al) adsorption of humic acids which were extracted from four composts. The functional groups were determined by Fourier transform infrared spectroscopy (FTIR) and solid‐state 13C nuclear magnetic resonance spectroscopy (13C‐NMR). The results showed that the aromatic groups were all found in the humic acids of the four composts, and the surface of humic acids included carboxylic group, hydroxylic group, and amino group. The experiment of Al adsorption was described by Freundlich equation. It showed that the adsorption of Al by humic acid from compost of cattle manure was higher, but that from the compost of pig manure, lemon manure, tea manure in equal preparation was lower. The adsorptive behavior was different due to the interaction by functional groups ( OH and COOH) with Al. These results can describe the fate and transportation of Al in the soil of different organic fertilizer. 相似文献
11.
乌梁素海沉积物腐殖质的组成及分布特征 总被引：4，自引：2，他引：2
以乌梁素海为研究对象,应用化学方法对乌梁素海湖泊表层沉积物有机质含量、腐殖质组成、影响腐殖质的环境因子及腐殖化程度进行了研究,结果表明:乌梁素海表层沉积物有机质含量变化范围为1.06%~5.38%.有机质分布特征与表层沉积物所处的水力条件、水生植物分布、人为活动有关;腐殖质组成中,胡敏酸含量为0.65~2.78 g/kg,富里酸含量为2.19~8.72 g/kg,胡敏素是主体,含量在3.28~20.92 g/kg之间;腐殖质与环境因子的相关性分析中,各组分含量与有机质含量呈极显著相关.pH值与胡敏素含量呈显著负相关.溶解氧与富里酸、胡敏素含量分别呈极显著正相关、显著正相关.腐殖化程度分析中,随着有机质含量的上升,可提取腐殖质的相对含量下降.PQ值介于9.50%~27.06%之间,平均值为21.47%,变异较大,且腐殖化程度较低. 相似文献
12.
After an explanation of the complex composition of the system of humic substances, some gravel deposits are described from whose open-pit boundaries there were taken water samples, their content of components of humic matter having been determined. Since spectroscopic analysis techniques yield spectra which are difficult to analyse and have a relatively low detection sensitivity, an indirect polarographic method was applied by which water-soluble humic substances can be determined very selectively down to a lower limit of 5 μg/1. Thus, enrichment in water or in adsorbing columns is not required. From the results found there are derived several statements concerning the occurrence of humic substances and fulvic acids in waters and conclusions about the possible influence on the hardening of concrete are given. 相似文献
13.
The Influence of pH and Calcium Concentration on Metal-Fulvic Acid Bonds – Stripping Voltammetric and Chemometric Investigations. Electrochemically available metal concentrations of Cd, Cu and Zn were analyzed in dependence of different concentrations of calcium, fulvic acids and pH by Differential Pulse Anodic Stripping Voltammetry (DPASV). A statistical experimental design was the base to minimize the number of experiments and to include the multifarious interactions between the independent variables (Ca concentration, concentration of fulvic acids and the pH value) and otherwise the electrochemically available metal concentrations of Cd, Cu and Zn. At first analysis of experimental data was carried out by multiple linear regression. The main influence on available metal concentrations is the pH value especially for Cu. The Ca ion influence has not competitive effect and is only significant for Cd and Cu. It has an effect on the metal-fulvic acid-bonds. Two factor interactions exist for pH/fulvic acids and for Ca/fulvic acids too. The partial least squares regression (PLS) model was used to include the interactions between the metals Cd, Cu and Zn. For proving these two models the three parameters pH, Ca and fulvic acid were varied within the calibration range of the models and predicted values were compared with the experimental values. The approach with the PLS model is better than the approach with the multiple linear regression (normally used mathematical method of analysis of a factorial plan) with a relative error of 9.7% for modelling of Cd, 6.0% for Zn and 58% for Cu in relation to multiple linear regression with errors of 12% for Cd, 8.6% for Zn and 65% for Cu. The PLS modelling is a suited tool for modelling chemical interactions also in simulated natural matrices. 相似文献
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15.
Stefania Mazzuoli Steven Loiselle Vincent Hull Luca Bracchini Claudio Rossi 《洁净——土壤、空气、水》2003,31(6):461-468
The spatial and temporal distribution of humic substances in aquatic ecosystems can have important effects on ecosystem productivity, negatively impacting primary productivity while positively impacting secondary productivity. In the present investigation, a large shallow lake ecosystem was studied to determine the spatial and seasonal variation of the composition and concentration of humic substances. Concentrations of total dissolved organic matter, humic acid, and fulvic acid were found to display significant spatial distributions (1.3…13.5 mg/L, DOM; 0.1…5.4 mg/L, HA). The distribution is described by using mapping techniques and the analysis of the spatial distribution of the lake. An analysis of the seasonal variations also indicated the dependence of the occurrence of these compounds on meteorological and hydrological conditions. To identify the potential sources of these organic materials, an analysis was made of the ratio of humic and fulvic acid fractions and total DOM. It was found that areas of high DOM concentration coincided with the areas of highest HA percentage of total DOM. Furthermore using the ratio of the normalised concentrations of HA, FA, and residual DOM (< 5000 g/mol) it was found that areas dominated by each are spatially distinct. This confirms the hypothesis that in these shallow lakes, photodegradation and bacterioplankton activity will create a residence time dependent zonation of each component of the total DOM. 相似文献
16.
Frequent heavy rainfalls during the East Asian summer monsoon drastically increase water flow and chemical loadings to surface waters. A solid understanding of hydroclimatic controls on watershed biogeochemical processes is crucial for water quality control during the monsoon period. We investigated spatio‐temporal variations in the concentrations and spectroscopic properties of dissolved organic matter (DOM) and the concentrations of trace metals in Hwangryong River, Korea, during a summer period from the relatively dry month of June through the following months with heavy rainfall. DOM and its spectroscopic properties differed spatially along the river, and also depended on storm and flow characteristics around each sampling time. At a headwater stream draining a forested watershed, the concentrations (measured as dissolved organic carbon (DOC)), aromaticity (measured as specific UV absorbance at 254 nm), and fulvic acid‐ and protein‐like fluorescence of DOM were higher in stormflow than in baseflow waters. DOC concentrations and fluorescence intensities increased along the downstream rural and urban sites, in which DOC and fluorescence were not higher in stormflow waters, except for the ‘first flush’ at the urban site. The response of DOM in reservoir waters to monsoon rainfalls differed from that of stream and river waters, as illustrated by storm‐induced increases in DOM aromaticity and fulvic‐like fluorescence, and no significant changes in protein‐like fluorescence. The results suggest that surface water DOM and its spectroscopic properties differentially respond to changes in hydroclimatic conditions, depending on watershed characteristics and the influence of anthropogenic organic matter loadings. DOC concentrations and intensities of spectroscopic parameters were positively correlated with some of the measured trace metals (As, Co, and Fe). Further research will be needed to obtain a better understanding of climate effects on the interaction between DOM and trace metals. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
17.
Humic compounds are one of the major substances that affect the COD value of the surface water of Lake Kojima. The humic compounds in the lake water were studied with a spectrofluorophotometer. The 3-dimensional fluorescence spectrum suggests that two kinds of fluorescent substances exist in the lake water. One shows an fluorescence excitation maxim (Ex) at about 240 nm and an emission maxim (Em) at 413 nm. The other has an Ex at 330 nm and an Em at 418 nm. The former peaks are associated with humic acid and the latter peaks are associated with fulvic acid. These peak intensities are proportional to the COD values. The fulvic acid in the lake water may be enriched by the effluent from domestic water treatment plants. 相似文献
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Edward R. Sholkovitz 《Earth and Planetary Science Letters》1978,41(1):77-86
A laboratory experiment was carried out in which the flocculation products, formed from the mixing of filtered (0.4 μm) river water and seawater, were analysed. This study established that Fe, Mn, Al, Cu, Ni, Cd and Co have resolvable and well-defined estuarine chemistries. Copper, Ni, Mn and Co have salinity dependences of removal which are similar to those of dissolved Fe and humic acids. The amount of removal of the above trace metals increases between 0 and 15–18‰, after which little additional removal occurs. The extents of removal from river water are very different: Fe, 95%; Al, 20%; Cu, Ni, 40%; Co, 10%; Cd, 5% and Mn, 25–45%. The basic removal mechanism appears to be the estuarine flocculation of trace metals which exist, in part, in river water as colloids in association with colloidal humic acids and hydrous iron oxides. A qualitative model, based on this mechanism, supports the observations of this flocculation study.The results of this study give the most complete and consistent set of data presently available, from which to postulate the most important processes controlling the estuarine chemistry of trace metals. The generality of their behaviours still needs to be determined by future investigations. 相似文献
20.
Colloidal materials and macromolecules that are less than 0.45 mircons in diameter can act to transport both hydrophobic and hydrophilic contaminations in subsurface waters. An automated hollow-fibre ultrafiltration apparatus is described and evaluated regarding its ability to physically size and isolate colloidal materials from natural waters. Sufficient size-fractionated colloidal samples can be obtained from 60 litre samples to perform inorganic, organic, and radionuclide characterizations, as well as allow the binding capacities of humic and fulvic materials to be evaluated. Samples processed in the field with the automated system were compared with laboratory analyses using single cartridge hollow-fibre ultrafilters and found to agree well with each other. Typical sample reproducibilities were with 15 per cent. The results indicate that field collection of colloidal materials is feasible with minimal sample perturbation using the automated sampler. 相似文献