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1.
Lack of filtration and rapid transport of groundwater and particulate matter make karst aquifers susceptible to bacterial contamination. This study utilized quantitative polymerase chain reaction (qPCR) to examine the transport and attenuation of two nonvirulent isolates of Escherichia coli (E. coli) in relation to traditional groundwater tracers (rhodamine WT dye and 1-µm diameter latex microspheres) in a karst-conduit aquifer in central Kentucky. Bacterial isolates were labeled with stable isotopes (15N and 13C). All tracers were detected more than 6 km downstream from the injection site and demonstrated overlapping breakthrough curves, with differential transport observed between the two bacterial strains. The E. coli isolate containing the kps gene (low attachment) arrived at sampling sites 1.25 to 36 h prior to the bacterial isolate containing the iha gene (high attachment) and was detected in samples collected following storm events in which the iha isolate was not detected. The storage potential of contaminants within karst systems was demonstrated by the remobilization of all tracers during storm events more than 1 month after injection. Bacteria-sized microspheres were more easily remobilized during periods of increased discharge compared to other tracers. The study demonstrated that molecular biology techniques such as qPCR can be utilized as a sensitive analysis of bacterial tracers in karst aquifers and may prove to be a more sensitive analytical technique than stable isotope analysis for field-scale traces.  相似文献   

2.
Karst aquifers are susceptible to contamination by microorganisms, but relatively few studies have used bacteria as tracers. We demonstrate the utility of Escherichia coli enriched in the stable isotope nitrogen‐15 (15N) as a novel bacterial tracer. Nonpathogenic E. coli from two springs in central Kentucky were grown on 15N‐enriched media. Survival of E. coli and persistence of the isotopic signal were assessed in two sets of laboratory experiments conducted with sterilized spring water in dark microcosms at 14 °C. First, isotopically labeled bacteria survived for 130 d at concentrations within one log unit of the average initial value, and there was no significant difference in δ15N values from Day 1 to Day 130. Second, water samples with E. coli were inoculated with either of two different species of protozoa (Tetrahymena pyriformis or Colpoda steinii). During 7 d, δ15N values increased in T. pyriformis while bacterial populations decreased. In a field test, following a 2.1‐cm rainfall, 15N‐labeled E. coli, solutes (rhodamine WT dye and bromide), and latex microspheres were injected into a sinkhole approximately 530 m upgradient of a spring. Breakthrough of all tracers coincided, but microspheres were remobilized by subsequent storms, unlike other tracers. Enriched E. coli exhibited more tailing than solute tracers during the initial storm‐flow recession. These results indicate that 15N‐enriched E. coli is a viable tracer of bacterial transport in karst aquifers, although predation may attenuate the isotopic signal in systems that are not rapidly flushed.  相似文献   

3.
A total of 120 samples comprising of water (45), sediment (45) and mangrove originated food (30) collected from mangrove ecosystems of Goa were screened for Escherichia coli employing ISO-16654 method. Seventy-one (59.16%) samples were positive for E. coli. The E. coli isolates were further characterized by serotyping, virulence gene profiling and pulsed field gel electrophoresis (PFGE). Water and sediment samples were analyzed for physico-chemical parameters. The serotypes reported were O1, O10, O13, O17, O36, O41, O50, O68, O105, O116, O141, O148, O159, O162 and rough types while, 23 strains could not be typed. The stx1 and stx2 genes were detected in 33(46.47%) and 16(22.53%) isolates, respectively. The XbaI restriction digestion patterns of the stx positive strains were diverse. Interestingly, few strains isolated from diarrheal patients and from water, sediment and food from mangrove sources were genetically similar. The study showed that the mangrove ecosystem could be a potential reservoir for pathogenic E. coli.  相似文献   

4.
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5.
This article describes laboratory batch sorption and column transport experiments that were conducted using heterogeneous alluvial sediments with a wide physical characteristic from wells, located between Lake Mogan and Lake Eymir, Gölbaşı, Ankara. The batch sorption experiment was conducted in two separate systems, that is, single and multicomponents. Single batch experiment was performed to determine equilibrium condition between the heavy metal ions and the soil adsorption sites. The sorption isotherms data from multibatch experiments were used to calculate the sorption parameters. Single batch experiment indicated that equilibrium was attained within 9 days from the start of the sorption test. As a result of multicomponents batch experiments, for Zn and Mn, the sorption process was well described by the Freundlich or Langmuir isotherm model, whereas sorption of Cu was better described by the linear isotherm model. The Kd of Cu were found to be highest in soil 1 (32550.350 L kg−1) and lowest in soil 5 (18170.76 L kg−1). The maximum and minimum sorption capacity values for Zn were found to be in soil 1 (10985.148 mg kg−1) and in soil 2 (8597.14 mg kg−1) units, respectively. [Correction added after online publication 15 July, 2010: In the preceding sentence, the words “minimum” and “maximum” were initially switched.] Similarly, soil 1 (7587.391 mg kg−1) and soil 5 (4908.695 mg kg−1) units provided the maximum and minimum values for Mn. In the column experiments, flow and tracer transport was studied under saturated conditions using conservative tracer to determine the transport parameters. Transport parameter values were obtained by curve-fitting using the nonlinear least-squares optimization code CXTFIT. Results of the column experiments indicated that the dispersivity values obtained for soil samples were in the range of 0.024 to 1.13 cm.  相似文献   

6.
Song‐Bae Kim 《水文研究》2005,19(20):4069-4079
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7.
Adsorption of reactive black 5 (RB5) from aqueous solution onto chitosan was investigated in a batch system. The effects of solution pH, initial dye concentration, and temperature were studied. Adsorption data obtained from different batch experiments were modeled using both pseudo first‐ and second‐order kinetic equations. The equilibrium adsorption data were fitted to the Freundlich, Tempkin, and Langmuir isotherms over a dye concentration range of 45–100 µmol/L. The best results were achieved with the pseudo second‐order kinetic and Langmuir isotherm equilibrium models, respectively. The equilibrium adsorption capacity (qe) was increased with increasing the initial dye concentration and solution temperature, and decreasing solution pH. The chitosan flakes for the adsorption of the dye was regenerated efficiently through the alkaline solution and was then reused for dye removal. The activation energy (Ea) of sorption kinetics was estimated to be 13.88 kJ/mol. Thermodynamic parameters such as changes in free energy (ΔG), enthalpy (ΔH), and entropy (ΔS) were evaluated by applying the van't Hoff equation. The thermodynamics of reactive dye adsorption by chitosan indicates its spontaneous and endothermic nature.  相似文献   

8.
Cryptosporidium parvum is a protozoan parasite, transmitted through aqueous environments in the form of an oocyst. In this study, a transport model into which sorption, filtration and inactivation mechanisms are incorporated is applied to simulate laboratory column data, and the suitability of a kinetic model to describe the C. parvum oocyst transport and removal in porous media is compared with an equilibrium model. The kinetic model is applied to simulate previous column experimental data and successfully simulates the concentration peak; the late time tailing effect appeared in the breakthrough curves, indicating that the kinetic model is more suitable than the equilibrium one at simulating the fate and transport of the oocysts in porous media. Simulation illustrates that sorption causes retardation along with a tailing in the breakthrough curve. Additionally, filtration acts as a major mechanism of removing the oocysts from the aqueous phase, whereas the role of inactivation in reducing the viable oocyst concentration is minimal. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

9.
In this study, we tested a practical strategy useful for accurate chlorinated volatile organic compound (cVOC) sorption prediction. Corresponding to the feature of the superposition of adsorption due to thermally altered carbonaceous matter (TACM) with organic carbon‐water partitioning, a nonlinear Freundlich sorption isotherm covering a wide range of aqueous concentrations was defined by equilibrium sorption measurement at one or a few low concentration points with extrapolation to the empirical organic carbon‐water partition coefficient (Koc,e) near compound solubility. We applied this approach to obtain perchloroethene equilibrium sorption isotherm parameters for TACM‐containing glacial sand and gravel subsoil samples from a field site in New York. Sorption and associated Koc,c applicable to low (5–500 µg/L) and high (>100,000 µg/L) aqueous concentrations were determined in batch experiments. (The Koc,c is the organic carbon‐normalized sorption partition coefficient corresponding to aqueous concentration Cw.) The Koc,c measurements at low concentration (~5 µg/L) were 6 to 34 times greater than the Koc,e. The importance of this type of data is illustrated through presentation of its substantial impact on the site remedy. In so doing, we provide an approach that is broadly applicable to cVOC field sites with similar circumstances (low carbon content glacial sand and gravel with TACM).  相似文献   

10.
Sustainable water management in semi-arid agriculture practices requires quantitative knowledge of water fluxes within the soil-vegetation-atmosphere system. Therefore, we used stable-isotope approaches to evaluate evaporation (Ea), transpiration (Ta), and groundwater recharge (R) at sites in Senegal's Groundnut basin and Ferlo Valley pasture region during the pre-monsoon, monsoon, and post-monsoon seasons of 2021. The approaches were based upon (i) the isothermal evaporation model (for quantifying Ea); (ii) water and isotope mass balances (to partition Ea and Ta for groundnut and pasture); and (iii) the piston displacement method (for estimating R). Ea losses derived from the isothermal evaporation model corresponded primarily to Stage II evaporation, and ranged from 0.02 to 0.09 mm d−1 in the Groundnut basin, versus 0.02–0.11 mm d−1 in Ferlo. At the groundnut site, Ea rates ranged from 0.01 to 0.69 mm d−1; Ta was in the range 0.55–2.29 mm d−1; and the Ta/ETa ratio was 74%–90%. At the pasture site, the ranges were 0.02–0.39 mm d−1 for Ea; 0.9–1.69 mm d−1 for Ta; and 62–90% for Ta/ETa. The ETa value derived for the groundnut site via the isotope approach was similar to those from eddy covariance measurements, and also to the results from the previous validated HYDRUS-1D model. However, the HYDRUS-1D model gave a lower Ta/ETa ratio (23.2%). The computed groundwater recharge for the groundnut site amounted to less than 2% of the local annual precipitation. Recommendations are made regarding protocols for preventing changes to isotopic compositions of water in samples that are collected in remote arid regions, but must be analysed days later. The article ends with suggestions for studies to follow up on evidence that local aquifers are being recharged via preferential pathways.  相似文献   

11.
Time patterns of karst denudation in northwest Georgia (U.S.A.) were investigated at three spring sites for 12 months and at five stream sites for 10 years. Rainfall was evenly distributed and showed no significant seasonality. At the springs, as well as the streams, water hardness was largely controlled by discharge. At the springs, soil pCO2 and water pH were strongly correlated (r + -0·69 to -0·83). Solute transport in spring waters was highly seasonal, with two conduit flow springs removing more limestone in the winter, and the diffuse flow spring removing more during the growing season. At the stream sites, most denudation occurred during the winter and spring seasons, and least during the summer. Fourier analysis showed that variations in denudation occur on deterministic (long-wave) as well as stochastic (shortwave) time scales. As contributing variables, discharge varied in short-wave and long-wave cycles, whereas soil pCO2 showed only a long-wave cycle. The 12 month deterministic cycles were the most important, with changes in discharge taking precedence over soil pCO2. Time series regression explains up to 69 per cent of changes in denudation through rain and soil pCO2. Time cycles in available water are the key controlling factor of denudation, and amounts of available soil CO2 may not be as important in the temporal patterns of karst downwearing as has been believed previously.  相似文献   

12.
This paper addresses the question of how spatial variability in the hydraulic and chemical properties of groundwater systems affects the transport and sorption behavior of pollutants at the field scale. In this paper, we limit our investigations on pollutants that adsorb according to an equilibrium controlled nonlinear Freundlich sorption isotherm. The new contribution of this paper is take into account not only spatially variable Freundlich distribution coefficients KSKS but spatially variable Freundlich nonlinearity parameters p as well. Using a homogenization theory approach, we shortly review the impact of spatially variable hydraulic properties on the transport and extend the theory to spatially variable chemical properties. We show that spatially variable Freundlich exponents cause a very different field scale transport and sorption behavior than spatial variations in the distribution coefficients only since in the first case field scale Freundlich parameters and field scale dispersion coefficients become concentration dependent. In particular, field scale retardation is much larger than small-scale retardation.  相似文献   

13.
The estimation of evapotranspiration (E) in forested areas is required for various practical purposes (e.g. evaluation of drought risks) in Japan. This study developed a model that estimates monthly forest E in Japan with the input of monthly temperature (T). The model is based on the assumptions that E equals the equilibrium evaporation rate (Eeq) and that Eeq is approximated by a function of T. The model formulates E as E (mm month−1) = 3·48 T ( °C) + 32·3. The accuracy of the model was examined using monthly E data derived using short‐term water balance (WB) and micrometeorological (M) methods for 15 forest sites in Japan. The model estimated monthly E more accurately than did the Thornthwaite and Hamon equations according to regression analysis of the estimated E and E derived using the WB and M methods. Although the model tended to overestimate monthly E, the overestimation could be reduced by considering the effect of precipitation on E. As T data are commonly available all over Japan, the model would be a useful tool to estimate forest E in Japan. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

14.
Abstract

This paper proposes a method for assessing the renewable groundwater reserves of large regions for an average year, based on the integration of the recession curves for their basins' springs or the natural baseflow of their rivers. In this method, the hydrodynamic volumes (or renewable reserves), were estimated from the baseflow. It was assumed that the flow was the same as the natural recharge, and the recession coefficients were derived from the hydrogeological parameters and geometric characteristics of the aquifers and adjusted to fit the recession curves at gauging stations. The method was applied to all the aquifers of Spain, which have a total renewable groundwater reserve of 86 118 hm3—four times the mean annual recharge. However, the spatial distribution of these reserves is highly variable; 18.6% of the country's aquifers contain 94.7% of the entire reserve.

Citation Sanz, E. & Recio, B. (2010) A method to assess annual average renewable groundwater reserves for large regions in Spain. Hydrol. Sci. J. 56(1), 99–107.  相似文献   

15.
In India, the annual production of tea is ca. 857,000 tonnes, which is 27.4% of the total world production. The amount of tea factory waste (TFW) produced per annum after processing is ca. 190,400 tonnes. TFW can be used as a low cost adsorbent for the removal of toxic metals from the aqueous phase. An investigation was carried out to study the feasibility of the use of TFW as an adsorbent for the removal of the heavy metal, zinc. Equilibrium, kinetic and thermodynamic studies were reported. The straight line plot of log (qe–q) versus time t for the adsorption of zinc shows the validity of the Lagergren equation. The various steps involved in adsorbate transport from the solution to the surface of the adsorbent particles were dealt with by using a Weber‐Morris plot, qe versus t0.5 for the TFW. The rate controlling parameters, kid,1 and kid,2, were determined and it was found that the macro‐pore diffusion rate was much larger than micro‐pore diffusion rate. A batch sorption model, which assumes the pseudo‐second‐order mechanism, was used to predict the rate constant of sorption, the equilibrium sorption capacity and the initial sorption rate with the effect of initial zinc (II) ion concentration. Equilibrium data obtained from the experiments were analyzed with various isotherms, i. e., Freundlich, Langmuir, Redlich‐Peterson and Tempkin. The adsorption equilibrium was reached in 30 min and the adsorption data fitted well to all models. The maximum adsorption capacity of TFW for zinc (II) ions was determined to be 14.2 mg/g. The capacity of adsorption on Zn(II) increased with increasing temperatures and pH. The maximum uptake level of zinc was observed at pH of 4.2. The various thermodynamic parameters, i. e., ΔG°, ΔH° and ΔS°, were estimated. The thermodynamics of the zinc ion/TFW system indicated a spontaneous, endothermic and random nature of the process. The results showed that the TFW, which has low economical value, is a suitable adsorbent for the removal of zinc (II) ions from aqueous solutions.  相似文献   

16.
Yang N  Chu DL  Wong MM  Qi H  Wu RS  Kong RY 《Marine pollution bulletin》2011,62(12):2654-2658
Marine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5′-noncoding region (5′-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichiacoli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters.  相似文献   

17.
In wind‐driven rains, wind velocity and direction are expected to affect not only energy input of rains but also shallow ?ow hydraulics by changing roughness induced by raindrop impacts with an angle on ?ow and the unidirectional splashes in the wind direction. A wind‐tunnel study under wind‐driven rains was conducted to determine the effects of horizontal wind velocity and direction on sediment transport by the raindrop‐impacted shallow ?ow. Windless rains and the rains driven by horizontal wind velocities of 6 m s?1, 10 m s?1, and 14 m s?1 were applied to three agricultural soils packed into a 20 by 55 cm soil pan placed on both windward and leeward slopes of 7 per cent, 15 per cent, and 20 per cent. During each rainfall application, sediment and runoff samples were collected at 5‐min intervals at the bottom edge of the soil pan with wide‐mouth bottles and were determined gravimetrically. Based on the interrill erosion mechanics, kinetic energy ?ux (Ern) as a rainfall parameter and product of unit discharge and slope in the form of qbSco as a ?ow parameter were used to explain the interactions between impact and ?ow parameters and sediment transport (qs). The differential sediment transport rates occurred depending on the variation in raindrop trajectory and rain intensity with the wind velocity and direction. Flux of rain energy computed by combining the effects of wind on the velocity, frequency, and angle of raindrop impact reasonably explained the characteristics of wind‐driven rains and acceptably accounted for the differences in sediment delivery rates to the shallow ?ow transport (R2 ≥ 0·78). Further analysis of the Pearson correlation coef?cients between Ern and qSo and qs also showed that wind velocity and direction signi?cantly affected the hydraulics of the shallow ?ow. Ern had a smaller correlation coef?cient with the qs in windward slopes where not only reverse splashes but also reverse lateral raindrop stress with respect to the shallow ?ow direction occurred. However, Ern was as much effective as qSo in the sediment transport in the leeward slopes where advance splashes and advance lateral raindrop stress on the ?ow occurred. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

18.
Partitioning evapotranspiration (ET) into evaporation (E) and transpiration (T) in wetlands is important for understanding the hydrological processes in wetlands and the contribution of wetland ET to local and regional water cycling and for designing effective wetland management strategies. Stable water isotopes are useful in the application of ET partitioning through the evaluation of the isotopic compositions of E (δE), T (δT), and ET (δET) obtained from observation or modelling methods. However, this approach still suffers from potentially large uncertainties in terms of estimating the isotopic endmembers. In this study, we modified the traditional isotope‐based ET partitioning methods to include leaf‐level biological constraints to separately estimate the relative contributions of T from Scirpus triqueter and Phragmites australis and the relative contributions of E from the standing surface water in a semiarid marsh wetland in northeastern China. The results showed that although the δT values of S. triqueter and P. australis were rather similar, the mean δT values of the 2 species were different from the values of δE, making it possible to distinguish the relative contributions of E and T through the use of isotopes. The simulation of leaf water using a non‐steady‐state model indicated obvious deviations in leaf water enrichment (δLb) from isotopic steady states for both species, especially during early mornings and evenings when relative humidity was highest. The isotopic mass balance showed that E accounted for approximately 60% of ET, and T from S. triqueter and P. australis each contributed approximately 20% to ET; this implied that the transpiration of one reed was equivalent to that of 5.25 individuals of S. triqueter. Using the estimated ratio of T to ET and the measured leaf transpiration, the total ET was estimated to be approximately 10 mm day?1. Using the NSS‐Tr method, the estimated ET was higher than the water loss calculated from the water level gauge. This indicated that the river water and surrounding groundwater were the sources of the marsh wetland, with a supply rate of 8.3 mm day?1.  相似文献   

19.
Flow‐through column experiments were carried out to investigate the influence of pH on the sorption of three phenols (2‐methyl‐4, 6‐dinitrophenol, 2, 4, 6‐trichlorophenol, pentachlorophenol) onto a natural sandy aquifer material collected from a bank filtration site of River Elbe, Germany. For the phenols investigated, an increase in sorption (retardation) with decreasing pH is observed indicating a stronger sorption of the neutral species in comparison to that of the anions formed by dissociation. The anions of 2‐methyl‐4, 6‐dinitrophenol and 2, 4, 6‐trichlorophenol do not show significant sorption. On the contrary, pentachlorophenol showed sorption not only in neutral form but also in ionic form significantly which should be taken into account while assessing the fate and transport of such compound. A linear model based on the degree of protonation (calculated from pH and pKa) can be used to resolve the apparent (observed) sorption coefficient (Kd, app) into its neutral (Kd, n) and ionised (Kd, i) components. Knowing pKa, Kd, n, and Kd, i the apparent sorption coefficient for pH values other than experimentally investigated can be predicted.  相似文献   

20.
ABSTRACT

A Ratio Method is presented, which dispenses with the classical curve-matching procedure for computing characteristics of Ideal, Leaky and Bounded aquifers. This simple method consists of selecting any three values of observed drawdown, (t0, s0), (t1, s1) and (t2, S2), such that t1 = 2 t0 and t2 = 100 t0. Then, using the ratios, s0/s 1 and s0/s2, the values of u0, W(u0) as well as r/B (in the case of leaky aquifers) and ri (in the case of aquifers with one boundary) are extrapolated from the charts presented. The extrapolated values are substituted in the standard equations to determine the aquifer characteristics. The Ratio Method may also have useful application in other branches of science where the curve-matching procedure is employed for calculation of parameters. (Key Words: Ratio Method; Aquifer characteristics; Ideal aquifer; Leaky aquifer; Bounded aquifer)  相似文献   

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