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1.
Diseased fish showing erosion of the fins have been recorded from polluted waters in several parts of the world. This article gives the results of a year's survey of fin erosion among fish in oceanic and estuarine waters of New York Bight. The pristine Great Bay was chosen as a control for the heavily contaminated Sandy Hook-Raritan Bay system. Almost all fin erosion was confined to demersal flat fish, particularly the winter flounder, a species which spends a long time in polluted estuaries near Sandy Hook.  相似文献   

2.
The results of the present study are based on enzyme biomarker measurements in flounder (Platichthys flesus), a flatfish species that is widely distributed in Baltic coastal waters. The fish were collected from known pollution gradients and from reference areas regarded as relatively free of anthropogenic input. Muscular cholinesterases (ChEs), hepatic glutathione S-transferase (GST), and hepatic ethoxyresorufin-O-deethylase (EROD) activities were measured in each sampled specimen of flounder. Generalized linear models (GLM) were used to analyze the dependence of the enzyme activity on sampling year and area as well as on the biological parameters of the fish. Statistically significant differences in the activities of the measured biomarkers were observed between reference and contaminated sites. ChEs and GST activities differed with gender. The results of this study suggest that the location and year of sampling have a significant impact on the activity of all the measured biomarkers.  相似文献   

3.
Effluent upgrades for metropolitan Boston have included toxicant reduction, primary and secondary treatment and outfall extension. Between 1992 and 2003 winter flounder at five stations were surveyed annually for liver and muscle burden and chronic hepatic sub-lethal impacts of polynuclear and halogenated aromatic hydrocarbons, and metals. Trends in flounder availability and fin condition were also examined. In 1988 12% of the adult winter flounder in Boston Harbor exhibited hepatic neoplasms and up to 80% had hepatic hydropic vacuolation (HV). Tumor prevalence fell to 0-2% and HV to <50% by 1996. Since then tumors have been absent, while a steady prevalence of HV has persisted, consistent with lower hydrocarbon loading and tissue levels. Contaminants and HV also fell with distance from the Boston outfall. After the outfall extension was activated in 2000, there has been no significant change in flounder liver health at the new outfall site.  相似文献   

4.
In this study, individual Japanese flounder were intraperitoneally injected with 2 μg tributyltin-d27 (TBT-d27) fish−1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.  相似文献   

5.
We investigated levels of the pollutant tributyltin (TBT) in blood of pufferfishes (six species), Japanese sea perch, red sea bream, Japanese common goby, Japanese flounder, rockfish, conger eel, and sea mullet collected off the coast of northern Kyushu, Japan. We found considerable levels of TBT (1.4-190 ng/mL) accumulated in the blood of these fish. Blood TBT concentrations were 1.3-22.5 times liver concentrations and 4.9-78 times muscle concentrations, except in conger eel and mullet. We detected TBT (16-111 ng/mL-blood) in the plasma of the fine-patterned puffer (Takifugupoecilonotus) year-round, without any apparent seasonal trend. These results suggest that fish inhabiting coastal areas of Kyushu, Japan, continue to be contaminated with TBT.  相似文献   

6.
To investigate whether the biological toxicity of aquatic hypercapnia is due to the direct effects of CO2 or to the effects of acidification of seawater by CO2, the Japanese flounder (Paralichthys olivaceus) was subjected to seawater equilibrated with a gas mixture of air containing 5% CO2 (pH 6.18) or seawater acidified to the same pH with 1 N H2SO4. All the fish died within 72 h in the CO2 exposure group, whereas no mortality occurred in the acid group. Acid-base parameters as well as plasma ion concentrations were severely perturbed in the CO2 exposure group, whereas they were minimally affected in the acid group. These results clearly demonstrate that the mortality in the CO2 group is a direct result of the elevated levels of dissolved CO2 and not to the effects of the reduced water pH.  相似文献   

7.
Results are presented of a study on liver histopathology in Baltic flounder (Platichthys flesus) carried out in 2001 and 2002 in four coastal sampling areas of the Baltic Sea: Kv?d?fj?rden (Swedish east coast, reference area), Klaipeda-Butinge (Lithuanian coast), Gulf of Gdansk (Polish coast), and Wismar Bay (German coast) within the framework of the EU-funded BEEP project. Liver lesions were diagnosed and categorised using standardised methodologies and, for a spatial and temporal assessment of the prevalence and types of lesions detected, a scoring system was applied, involving the calculation of mean histopathology lesion scores. 83.0% of the 436 female flounder examined (size range: 20-43 cm total length, age range: 2-8 years) were affected by liver lesions, out of which 74.3% were assigned to the category of non-specific, 3.4% to the category of early toxicopathic non-neoplastic, 4.6% to the category of pre-neoplastic and 0.7% to the category of neoplastic lesions. Mean lesions scores were highest in the areas at the Lithuanian and Swedish coast and there is indication of an impact of the age structure of the flounder populations studied, the sampling season as well as of contaminant effects.  相似文献   

8.
Cellular changes in livers from flounder (Platichthys flesus L.) and digestive glands of blue mussels (Mytilus edulis) were studied to analyze whether the Elbe flood catastrophe in August 2002 had any deleterious effects on animal health. Flatfish and mussels were sampled at different locations in the Elbe estuary and the Wadden Sea after the flood wave had reached the German Bight. Internationally used core biomarkers (lysosomal membrane stability, biotransformation enzymes) were applied to assess the toxic effects of putatively increased pollution levels. In comparison to earlier data from long-term studies at the same stations, we found a significant impairment in the function of cell organelles (lysosomes) involved in the detoxification and elimination of pollutants in fish liver. Concentrations of relevant contaminants (organochlorines, polychlorinated biphenyls) were analyzed in parallel with cellular biomarkers, and conspicuously raised concentrations of insecticides metabolites were detected. Cell recovery and a clear reduction in contaminant concentrations were observed in fish livers five months after the flood at all sampling sites except the Helgolander Tiefe Rinne.  相似文献   

9.
Static bioassay tests were carried out on Channa punctatus Bloch in the laboratory after renewal of concentrations every 24 h. The LC50 values and 95 per cent confidence limits were 45.21 (39.62 … 51.11) ppm at 12 h and 29.8 (27.49 … 31.95) ppm of Zn at 96 h of exposure. The actual dissolution values of zinc were also determined in test solutions using an atomic absorption spectrophotometer. Behavioural changes were the higher rate of opercular movements, surfacing, loss of equilibrium and haemorrhage near mouth and caudal fin. The LT50 values indicate that survival was increased with decreasing Zn concentration. The histopathological changes observed due to Zn toxicity in the gills have been discussed in relation to fish mortality.  相似文献   

10.
Various brominated flame retardants (BFRs), including polybrominated diphenyl ethers (PBDEs) and current-use, non-PBDE BFRs, as well as organochlorine (OC) pesticides and polychlorinated biphenyls (PCBs), were measured in winter flounder, harp and hooded seals, and North Atlantic right whales from the Eastern United States and Canada. The concentrations of PBDEs in winter flounder and right whales were similar in magnitude to the levels of PCBs, which was unlike the pattern observed in seals. In these marine mammals, the levels of PBDEs were orders of magnitude lower than the levels of OCs and PCBs detected. Evidence existed for the accumulation of methoxylated (MeO)-PBDEs of natural origin in seals and right whales. Current-use, non-PBDE BFRs (including hexabromocyclododecane, pentabromoethylbenzene, hexabromobenzene, and pentabromotoluene) were detected in winter flounder and marine mammals. Future research should focus on monitoring PBDEs, current-use, non-PBDE BFRs, and MeO-BDEs of natural origin in marine organisms from Massachusetts and Cape Cod Bays.  相似文献   

11.
During field campaigns of the BEEP project (Biological Effects of Environmental Pollution in Marine Coastal Ecosystems) in 2001-2002, metabolites of polycyclic aromatic hydrocarbons (PAHs) were determined in bile samples from three fish species, flounder (Platichthys flesus), perch (Perca fluviatilis) and eelpout (Zoarces viviparus), from four separate areas in the Baltic Sea. Two determination methods were applied: fixed wavelength fluorescence (FF) for pyrene-type metabolites and high-performance liquid chromatography with fluorescence detection (HPLC). There was a good correlation between the FF method and 1-OH pyrene determined by HPLC. Normalisation of the FF data for absorbance at 380 nm or bile protein concentrations greatly increased variance in one third and decreased it in two thirds of the cases and resulted in a loss of significant differences (protein normalisation) between the sampling stations, but normalisation of the HPLC data had little effect on the results. The biliary PAH metabolite content was usually higher in males than in females. In perch and eelpout the biliary PAH contents were at similar levels, whereas in flounder the levels were lower. The sampling areas arranged in decreasing order of biliary PAH contents were: Wismar Bay > Gulf of Gdansk > Lithuanian coast > Kvadofj?rden (reference area). It is concluded that FF with un-normalised data is a reliable and simple method for monitoring purposes and only one sex of a selected species should be used.  相似文献   

12.
Severe chemical ozone loss has been detected in the Arctic in the winter and spring of 1995–96 by a variety of methods. Extreme reductions in column ozone due to halogen catalysed chemistry were derived from measurements of the Halogen Occultation Experiment (HALOE) on board the Upper Atmosphere Research Satellite in the Arctic vortex. Here, we discuss further aspects of the HALOE observations in the Arctic over this period. Potential problems, both in the data themselves and in the methodology of the data analysis are considered and the reason for the differences between the Arctic ozone losses deduced from HALOE data version 17 and 18 is analysed. Moreover, it is shown that HALOE measurements in the Arctic in winter and spring 1995–96 compare well with observations by other ground-based and satellite instruments.  相似文献   

13.
随着人类活动对自然生态系统的负面影响不断加剧,无创性生物多样性评估变得越来越重要。本研究旨在利用环境DNA宏条形码技术研究赣江下游南昌段鱼类多样性,并从不同季节（春、夏、秋、冬）、不同水层（上层、中层和下层）和不同取样位置（近岸和离岸）比较鱼类环境DNA信息的物种组成和多样性。结果表明：利用环境DNA宏条形码技术在赣江下游南昌段检测到鱼类114种,其中83种为历史记录种。不同季节的鱼类环境DNA信息的多样性和组成显示出极显著差异。上层水检测到的鱼类物种数分别显著多于中层水和下层水,且中层水和下层水检测到的鱼类在上层水中绝大多数都被检测到。上层水、中层水和下层水的鱼类环境DNA信息的多样性和组成不具有显著性差异。近岸检测到鱼类物种数多于离岸的,鱼类多样性指数无显著性差异,但群落结构具有显著性差异。RDA分析表明,赣江下游鱼类环境DNA受温度和pH的影响较大。本研究能够为基于环境DNA宏条形码的赣江鱼类资源的调查提供基线数据,并对后续赣江鱼类资源环境DNA宏条形码监测实施不同目的的采样策略提供依据;可为使用环境DNA宏条形码技术研究流水系统鱼类多样性提供技术参考,为环境DNA宏条形码技术应...  相似文献   

14.
Although littoral regions in northern lakes may sustain fish spawning grounds, little is known of the temporal or spatial aspects of their thermal and chemical regimes. This shortcoming is due in part to the difficulty in properly sampling these regions throughout the year with adequate spatial resolution. This problem is especially critical in lakes affected by episodic acidification during the spring snowmelt, a period of substantial importance to fisheries recruitment. A device was constructed to alleviate the problems associated with conventional water quality sampling of littoral regions. Constructed of thick walled polyvinyl chloride and permanently installed in the nearshore regions of an Adirondack lake, the episodic event sampler (EES) provided finely resolved (0.25 m) temperature and water chemistry data during the spring snowmelt period (February to May) of 1989. Although the construction and installation of the sampler represented a significant investment in labour and materials, this was offset by the high quality of the data collected. As the samplers were relatively undamaged by freeze-thaw activity, it is expected that they will continue to provide excellent information for several years.  相似文献   

15.
Biomarkers of exposure and effect of pollutants were analyzed in croakers Micropogonias furnieri (Teleostei: Sciaenidae) captured in winter and summer in a polluted and in a non-polluted site at the Patos Lagoon estuary (Southern Brazil). Catalase and glutathione S-transferase activities (exposure biomarkers) and lipid peroxidation (effect biomarker) were analyzed in liver samples. Other two effect biomarkers were also studied: blood cells DNA damage (through comet assay and micronucleus test) and respiratory burst measurements. In a broad view, results point to an important seasonal variation of the biochemical biomarkers analyzed. However, data obtained clearly indicate that croakers collected in winter at the polluted site were subjected to a level of clastogenic agents sufficient to generate irreversible genetic damages (mutations) and impair the fish immune system.  相似文献   

16.
Wildfire has been shown to increase erosion by several orders of magnitude, but knowledge regarding short‐term variations in post‐fire sediment transport processes has been lacking. We present a detailed analysis of the immediate post‐fire sediment dynamics in a semi‐arid basin in the southwestern USA based on suspended sediment rating curves. During June and July 2003, the Aspen Fire in the Coronado National Forest of southern Arizona burned an area of 343 km2. Surface water samples were collected in an affected watershed using an event‐based sampling strategy. Sediment rating parameters were determined for individual storm events during the first 18 months after the fire. The highest sediment concentrations were observed immediately after the fire. Through the two subsequent monsoon seasons there was a progressive change in rating parameters related to the preferential removal of fine to coarse sediment. During the corresponding winter seasons, there was a lower supply of sediment from the hillslopes, resulting in a time‐invariant set of sediment rating parameters. A sediment mass‐balance model corroborated the physical interpretations. The temporal variability in the sediment rating parameters demonstrates the importance of storm‐based sampling in areas with intense monsoon activity to characterize post‐fire sediment transport accurately. In particular, recovery of rating parameters depends on the number of high‐intensity rainstorms. These findings can be used to constrain rapid assessment fire‐response models for planning mitigation activities. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

17.
洱海硅藻群落结构的时空分布及其与环境因子间的关系   总被引：4，自引：2，他引：2  
于2004-2005年间对洱海水体的硅藻群落进行逐月监测及研究,共发现71个种,分属于18个属.硅藻群落结构的季节变化显著,主要优势种为耐营养的属种.不同季节的硅藻优势种类有明显区别,冬季的主要优势种为Fragilaria crotonensis,春季Aulacoseira ambigua与F.crotonensis的组合占优势地位,夏季以Cyclotella ocellata为主,秋季则A.ambigua与Cyclostephanos dubius组合为优势种.空间上除1#点外,硅藻分布虽然在相对丰度上存在一定的南北差异,但优势属种在全湖具有较好的一致性.说明洱海全湖的水质都已处于中富营养状态.对除1#点外的11个采样点的硅藻及水化学数据进行平均,得到逐月数据,通过数理统计分析的手段,探讨硅藻群落变化与环境因子之间的关系,结果表明影响季节尺度硅藻群落发生变化的最主要因子是气象条件,其次是营养盐.  相似文献   

18.
The relationship between chemical exposure and disease outbreak in fish has not been fully defined due to the limitations of experimental systems (model fish and pathogens). Therefore, we constructed a system using the Japanese flounder, Paralichthys olivaceus, and viral haemorrhagic septicemia virus (VHSV), and evaluated it by heavy oil (HO) exposure. The fish were exposed to HO at 0.3, 0.03, 0.003, and 0 g/L following VHSV infection at doses of 10(2.5) or 10(3.5) tissue culture infectious dose (TCID)50/fish. As a result, groups given the dual stressors showed more than 90% mortality. Although VHSV infection at 10(2.5) and 10(3.5) TCID50/fish without HO exposure also induced high mortality, at 68.8% and 81.3%, respectively, HO exposure induced faster and higher mortality in the virus carrier fish, indicating that chemical stressors raise the risk of disease outbreak in fish. The experimental system established in this study could be useful for chemical risk assessment.  相似文献   

19.
Luc Rock  Bernhard Mayer 《水文研究》2007,21(24):3301-3315
The partially semi-arid Oldman River basin (OMRB), located in southern Alberta (Canada), has an area of 28 200 km2, is forested in its western headwater part, and is used for agriculture in its eastern part. Hydrometric measurements indicate that flow in the Oldman River has decreased by ∼34% between 1913 and 2003, and it is predicted that water withdrawals will increase in the next 20 years. The objective of this study was to determine whether isotope ratio measurements can provide further insight into the water dynamics of the Oldman River and its tributaries. Surface water samples were collected monthly between December 2000 and March 2003. Groundwater samples were taken from 58 wells during one-time sampling trips. Runoff within the OMRB is currently about 70 mm year−1, with a corresponding runoff ratio of 0·18. Seasonal flow characteristics are markedly different upstream and downstream of the Oldman River reservoir. Upstream, sharp increases in flow in late spring and early summer are followed by a rapid decrease to base flow levels. Downstream, a prolonged high flow peak is observed due to the storage effect of the Oldman River reservoir. The seasonal variation in the isotopic composition of surface water from upstream sites is small. This suggests that peak runoff is not predominantly generated by melting snow accumulated during the preceding winter, but mainly by relatively well-mixed young groundwater. A significant increase in the δ18O and δ2H values in the downstream part of the basin was observed. The increase in the isotopic values is partly due to surface water and groundwater influx with progressively higher δ18O and δ2H values in the eastern part, and partly due to evaporation. Hence, the combination of hydrometric data with isotope measurements yields valuable insights into the water dynamics in the OMRB that may be further refined with more intensive measurement programmes in the future. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

20.
Ground water discharge is often a significant factor in the quality of fish spawning and rearing habitat and for highly biologically productive streams. In the present study, water temperatures (stream and hyporheic) and seepage fluxes were used to characterize shallow ground water discharge and recharge within thestreambed of Catamaran Brook, a small Atlantic salmon (Salmo salar) stream in central New Brunswick, Canada. Three study sites were instrumented using a total of 10 temperature sensors and 18 seepage meters. Highly variable mean seepage fluxes, ranging from 1.7 x 10(-4) to 2.5 cm3 m(-2) sec(-1), and mean hyporheic water temperatures, ranging from 10.5 degrees to 18.0 degrees C, at depths of 20 to 30 cm in the streambed were dependent on streambed location (left versus right stream bank and site location) and time during the summer sampling season. Temperature data were usefulfor determining if an area of the streambed was under discharge (positive flux), recharge (negative flux), or parallel flow (no flux) conditions and seepage meters were used to directly measure the quantity of water flux. Hyporheic water temperature measurements and specific conductance measurements of the seepage meter sample water, mean values ranging from 68.8 to 157.9 microS/cm, provided additional data for determining flux sources. Three stream banks were consistently under discharge conditions, while the other three stream banks showed reversal from discharge to recharge conditions over the sampling season. Results indicate that the majority of the water collected in the seepage meters was composed of surface water. The data obtained suggests that even though a positive seepage flux is often interpreted as ground water discharge, this discharging water may be of stream water origin that has recently entered the hyporheic zone.The measurement of seepage flux in conjunction with hyporheic water temperature or other indicators of water origin should be considered when attempting to quantify the magnitude of exchange and the source of hyporheic water.  相似文献   

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