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1.
为研究饲料中添加β-胡萝卜素对中华绒螯蟹成体雌蟹育肥效果的影响,分别在饲料中添加不同浓度的人工合成β-胡萝卜素,配制五组实验饲料(β-胡萝卜素测定含量分别为3.3、56.7、108.9、207.9和320.0 mg/kg)投喂生殖蜕壳后雌蟹,评价了饲料中添加β-胡萝卜素对增重率(WGR)、性腺指数(GSI)、肝胰腺指数(HSI)、常规生化组成、抗氧化和免疫指标、色泽参数和类胡萝卜素含量的影响。结果显示:(1)饲料中添加人工合成β-胡萝卜素对雌蟹育肥期间的WGR、GSI和HSI均无显著影响,但饲料中添加β-胡萝卜素显著增加了中华绒螯蟹肝胰腺中总脂含量;(2)就抗氧化和免疫指标而言,肝胰腺和血淋巴中SOD、T-AOC、GSH-Px、AKP和ACP免疫相关酶活均随着饲料中β-胡萝卜素添加量的增加呈“先增加后降低”的变化趋势,饲料中β-胡萝卜素含量为100～200 mg/kg时,组织中抗氧化和免疫相关酶活最高;(3)卵巢、肝胰腺和蟹壳中虾青素和β-胡萝卜素含量均随着饲料中β-胡萝卜素添加量的增加而显著增加,但饲料中添加β-胡萝卜素仅显著增加了卵巢和肝胰腺红度值(a*)。综上,饲料中添加人工合成... 相似文献
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光照、温度和营养盐对三株盐生杜氏藻生长和色素积累的影响 总被引：5，自引：0，他引：5
通过正交试验研究了光照、温度和营养盐对3株盐生杜氏藻株系(BJ,OUC38和OUC8)生长和色素积累的影响,旨在探明适于盐生杜氏藻生长和β-胡萝卜素积累的光照、温度和营养盐条件,并对实验中3株株系生长和β-胡萝卜素积累速率进行了初步的比较。结果表明,盐生杜氏藻生长的最适光照和温度分别是145.3μmol.m-2.s-1和23℃;适于β-胡萝卜素积累的光照和温度分别是218.2μmol.m-2.s-1和23℃;适于生长的营养盐条件是NaNO30.4 mmo/L、尿素0.6 mmol/L和NaHCO318 mmol/L,而当NaNO3、尿素和NaHCO3浓度分别为0,0.2和18 mmol/L时有利于β-胡萝卜素的积累。高光照、低温有利于杜氏藻细胞β-胡萝卜素的积累,在218.2μmol.m-2.s-1光强和15℃条件下藻细胞β-胡萝卜素积累平均值可分别达10.39和12.46 pg/cell。3个株系中OUC38积累β-胡萝卜素的能力高于另外2株,但差异不显著。 相似文献
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盐藻和β-胡萝卜素研究述评 总被引：9，自引：1，他引：9
养殖盐藻生产β-胡萝卜素是近十几年新兴起的研究领域，因其应用前途广内，现已成为微藻生物学的研究前沿和热点，预计本世纪末和下世纪初将有更大发展。鉴于我国在这方面文章不系统、不全面，本文从盐藻生物学、养殖学、β-胡萝卜素异构体、β-胡萝卜素形成生理等角度全面概述了该领域的研究现状，并提出存在的问题与研究方向。 相似文献
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杜氏藻的RF-RAPD分子系统学分析 总被引：1，自引：0，他引：1
杜氏藻(Dunaliella)是绿藻门、绿藻纲、团藻目、多毛藻科中的一个属(也有单独归为杜氏藻目、杜氏藻科、杜氏藻属[1]).因可以在高盐的极端环境中生存,也称之为盐藻.盐藻富含各种类胡萝卜素和甘油,其中β-胡萝卜素累积可达细胞干重的14%,具有较高的经济价值.另外盐藻无细胞壁,仅有一层糖蛋白和神经氨酸组成的外膜包裹,类似天然的原生质体,是真核细胞基因工程首选的受体之一.盐藻主要用来生产β-胡萝卜素,生产中发现β-胡萝卜素产量不稳定,推测种质差异因素与此有密切关系,因此分析不同种及种内品系及其与β-胡萝卜素含量相关性非常重要. 相似文献
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杜氏藻是目前生产天然β-胡萝卜素最好的商品化微藻,其β-胡萝卜素含量最高达细胞干重的14%(质量分数)。此外,通过紫外线或化学诱变剂处理,也能获得用来生产八氢番茄红素、番茄红素、叶黄素和玉米黄素等其他类胡萝卜素突变株。为使杜氏藻类胡萝卜素商业开发顺利进行,分析光、温度、pH值、营养、天敌等养殖条件对杜氏藻生长和类胡萝卜素积累的影响;比较大型开放池、循环池、回旋池、级联池、大罐、异养发酵罐及封闭式光合反应器等养殖方式的利弊;同时,总结培养液再循环、藻体采集、藻泥干燥、类胡萝卜素提取等藻体采收的有益经验。 相似文献
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研究了NaNO3对两种杜氏藻Dunaliella salina和Dunaliella parva生长速度、色素积累的影响和NaNO3的吸收规律,结果表明,NaNO3浓度为1.5mmol/L时,D.salina生长最快,最高密度76.4×104cell/mL;对照组为16.3×104cell/mL;低氮有利于D.salinaβ-胡萝卜素积累,β-胡萝卜素最大值105mg/g出现在0.5mmol/L NaNO3的样中,对照组为45mg/g;较高的NaNO3有利于叶绿素a的合成;藻液pH值开始3-5d急剧上升,后在波动中下降。对D.parva来说,NaNO3浓度为2.5mmol/L时生长最快,细胞最高密度为295×104cell/mL,对照组为15×104cell/mL;在实验范围内,NaNO3浓度越高,β-胡萝卜素含量越高,β-胡萝卜素最大值为37.5mg/g,叶绿素最大值为65mg/g;建立了两种杜氏藻对NaNO3利用的动力学方程。 相似文献
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盐藻SOD的盐适应性与物质积累的关系 总被引：4，自引：1，他引：3
实验分析了不同NaCl浓度处理下,盐藻SOD活性及其与细胞密度、β-胡萝卜素积累和蛋白质积累的关系.结果表明:在30～120 g/L的盐度范围内,随着NaCl浓度的提高,SOD活性极显著地增强,说明盐藻SOD是一种高盐适应酶.盐藻SOD活性与细胞密度及物质积累关系密切:在SOD活性为0.710×108 cells-1·min-1左右时,盐藻细胞密度、β-胡萝卜素和蛋白质的积累量都最高,而在SOD活性较低或较高时,盐藻细胞密度、β-胡萝卜素和蛋白质的积累量都较低.可能适当盐度下SOD的活动较好地保护了盐藻体内物质积累反应的进行. 相似文献
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This paper analyses the global tendency of the sea level rise (SLR) and its long term influence on the sea level upstream drainage cascade based on the example of the level’s variation in the Vistula Lagoon of the Baltic Sea compared to the other lagoons and coastal regions of the southeastern part of the Baltic Sea. A steady positive trend in the water level variations was revealed; its magnitude varies significantly depending on the time period. In general, during the 100–150 year period, the rate of the SLR in the lagoons and coastal areas of the Baltic Sea (1.7–1.8 mm per year) is close to the SLR rate in the World Ocean. In the second half of the 20th century, the increased rate of the SLR in the lagoons and marine areas became stronger (up to 3.6 mm per year in the Vistula Lagoon and in 1959–2006 in the sea and exceeded the rate of global ocean SLR). It dramatically increased at the end of the last century both in the lagoons and in the sea (up to 10.0–15.0 mm per year). This is the response not only to the global climate warming but it is likely that it is also a response to the changes of the climate driving forces that influence the regimes of the local wind and precipitation in the catchment. 相似文献
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Distribution of Pacific-origin water in the region of the Chukchi Plateau in the Arctic Ocean in the summer of 2003 总被引：6，自引：1，他引：6
1Introduction Besidestheprecipitationandriverdischarges,the watersinthePacificOceanandtheAtlanticOceanare thesourcesoftheArcticOceanwater.TheAtlantic waterenterstheArcticOceanviatheFramStraitand theBarentsSea.Foritsdenserfeatureduetohigh salinity,mostofitsinkstothenorthofSvaldbardand circulatesinallthedeepbasinsintheArcticOcean, formingthedeepandbottomwatersoftheArcticO- cean(Aagaardetal.,1985;Rudelsetal.,1999).The BeringStraitistheonlychannelforthePacificwater toflowintotheArcticOce… 相似文献
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Izvestiya, Atmospheric and Oceanic Physics - The Great Andaman–Sumatra earthquake (GASE) on December 26, 2004, with magnitude Mw of 9.2, occurred in the Indian Ocean near the northwestern... 相似文献
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Data on the contents and compositions of the hydrocarbons (HCs)—aliphatic (AHCs) and polycyclic aromatic (PAHs)—are provided
in comparison with the contents of the total organic carbon (Corg), the lipids in the particulates, and the Corg in bottom sediments. Particular attention has been paid to the distribution of the HCs in the water area of the Kravtsov
oil field. It has been established that the concentrations of AHCs in the water are governed by the content of particulates,
and the elevated AHC concentrations are confined to the coastal areas. In the water area of platform D-6, the sandy bottom
sediments were notable for the great variability of the HC concentrations, both laterally and from year to year. In the summer
of 2010, the content of AHCs averaged 40 μg/g (19% in the Corg), and that of PAHs, 23 ng/g. Natural seepage from the sediment mass is considered to be a source of HCs along with oil contamination. 相似文献
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Relationship between the path of the Kuroshio in the south of Japan and the path of the Kuroshio Extension in the east 总被引：2，自引：0，他引：2
A relationship between paths of the Kuroshio and Kuroshio Extension (KE) is investigated, using the satellite-derived altimetry
dataset of 1993–2008. When the Kuroshio takes the nearshore nonlarge meander path or typical large meander path and resultantly
goes through the deeper channel (about 2500 m) of the Izu-Ogasawara Ridge, the KE path adopts a relatively stable state with
the two quasi-stationary meanders. On the other hand, when the Kuroshio takes the offshore nonlarge meander path and then
passes over the shallower part of the Ridge (about 1000 m), the KE path tends to be convoluted, i.e., an unstable state. 相似文献
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1 古新-下渐新统含油气层系 古新-始新统母岩形成于滨海和湖泊沉积环境中,并在大量冲积源的参与之下,促进了含分散有机质混合类型砂-黏土质陆源岩层的堆积。在中国东北诸盆地中,含工业油气的白垩-早第三纪建造形成于开阔的浅水和深水湖泊环境中。与中国的盆地类似,在下第三系下部 相似文献
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Causes of the bottom hypoxia in the eastern part of the Gulf of Finland in the baltic sea 总被引：1，自引：0，他引：1
A. A. Maximov 《Oceanology》2006,46(2):185-191
The data on the bottom concentrations of dissolved oxygen in the eastern part of the Gulf of Finland obtained in 1923–1939 and in 1962–1989 were analyzed. No statistically reliable differences were found between the two periods studied. It was found that, during the 20th century, the fluctuations in the oxygen concentration were caused by the interannual variability of the winter severity and water salinity. A strong oxygen deficit in the summer was recorded after cold winters, when early freezing suppressed the vertical mixing, and/or in the years of a significant inflow of salt waters from the Baltic Sea with low oxygen contents. It is likely that the long-term dynamics of the oxygen concentration near the bottom in the open parts of the bay is determined by the large-scale variability of the hydrometeorological parameters. 相似文献
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The role of the small-size (SF; 0.1–0.5 mm) and large-size (LF; 0.5–20.0 mm) fractions in the biomass and abundance of mesozooplankton
(0.1–20.0 mm) was assessed using the database of samples obtained during the cruises of RV Akvanavt in the northeastern Black Sea in November 2000 and October 2006. The mesozooplankton was collected by means of Juday nets
(37/50, filtering gauze 160 μm) and Niskin bottles in two areas: (1) the shelf and continental slope (30–1480 m depth) and
(2) the deep sea (depths of more than 1500 m). The plankton net was considerably less effective in collecting the SF of the
mesozooplankton (by a factor of 30–36) than the Niskin bottles. When comparing the SF and LF, we estimated the abundance and
biomass of the SF in the samples obtained with the Niskin bottles. The abundance of the SF in the deep-sea area was 2.5 times
lower compared to the shelf and continental slope, and the LF abundance was 5.0 times lower in the same way. The abundance
of the SF constituted 88% of the total mesozooplankton on the shelf and continental slope, and 78% in the deep-sea area. The
biomass of the SF was higher as well on the shelf and continental slope. Meroplankton played a significant role in the SF
zooplankton abundance (0.5 × 103 + 0.16 ind. m−3) in this area. The SF grazing impact was 10% of the total mesozooplankton grazing on the shelf and continental slope, and
17% in the deepsea area. Appendicularia and nauplii of copepods had the greatest contribution to the mesozooplankton grazing
among the SF group. 相似文献