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1.
The seasonal fluctuation of the plasmodia ofMyxobolus gibelioi Wu et Wang, 1982 in the gill filaments of the allogynogenetic gibel carpCarassius auratus gibelio (Bloch) in a fish pond in Hubei Province of China was investigated from August 1999 to July 2000. A total of 445 fish was
examined; the overall prevalence of the plasmodium infection in the fish was 64.94% and mean abundance of plasmodia was 11.65±27.85
(mean±SD). Significant seasonal changes in prevalence and mean abundance, with higher levels of the plasmodia infection from
late spring to autumn, were observed.
This research was financially supported by the Projects for Knowledge Innovation of the Chinese Academy of Sciences (No. KSCX2-1-04
and No. 1999051) and the Science & Technology Commision of Wuhan City (No. 995004095G). 相似文献
2.
Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei 总被引：1，自引：0，他引：1
Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp, Litopenaeus vannamei, which had been domesticated in tanks for more than four generations. Phenotypic and genetic parameters were evaluated and fitted to an animal model. Realized response was measured from the difference between the mean growth rates of selected and control families. Realized heritability was determined from the ratio of the selection responses and selection differentials. The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW), and ranged from 0.21±0.08 to 0.37±0.06 for size traits. Genetic correlations of phenotypic traits were more variable (0.51–0.97), although correlations among various traits were high (>0.83). Across generations, BW and size traits increased, while selection response and heritability gradually decreased. Selection responses were 12.28%-23.35% for harvest weight and 3.58%–13.53% for size traits. Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01–0.38±0.11 for size traits. All phenotypic and genetic parameters differed between various treatments. To conclude, the results demonstrated a potential for mass selection of growth traits in L. vannamei. A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress. 相似文献
3.
The community dynamics and potential carbon ?xation of intertidal macroalgae were investigated monthly from April 2014 to April 2015 in the northwest coast of Yellow Sea. Seasonal variations in biomass and carbon ?xation were presented and showed close relationship with community structure.The carbon ?xation rate ranged from 0.48±0.13 mg C/(g FW ·d) to 4.35±0.12 mg C/(g FW ·d). Sargassum thunbergii, Chondrus ocellatus and Ulva intestinalis were three most in?uential species which contributed27%, 21.9% and 18.5% variation of carbon ?xation rate, respectively. Standing carbon stocks ranged from7.52 g C/m~2 to 41.31 g C/m~2, and estimated carbon stocks varied from 11.77 g C/m 2 to 96.49 g C/m~2. The larger dif ference between estimated and standing carbon stocks implied that more ?xed carbon was exported from the community in summer and autumn than in winter. This study suggested that intertidal macroalgal community could provide a potential function in carbon ?xation of coastal ecosystem. 相似文献
4.
This study deals with contribution of artificial food pellet and natural food to Chinese prawn (Penaeus orientalis) growth in a semiintensive culture pond. The prawn carbon consumption, budget, and the effects of some factors on the budget were investigated. The results showed that 26.2% of P. orientalis growth carbon came from formulated feed at the initial culture stage (when the prawns were 0.06±0.01 g in wet weight), and was 62.5% when the prawns were 9.56±1.04 g. The remaining part of the growth carbon was derived from organic fertilizer and natural food. The highest growth rate occurred at 20×10-3 salinity. Suitable salinity for culturing Chinese prawn was (20-28)×10-3. 相似文献
5.
The interspecific interactions between the rotifer Brachionus plicatilis and two harmful algal blooms (HAB) species were investigated experimentally by single culture method. B. plicatilis population and the growth of the two algae were compared at different algal cell densities. The results demonstrated that
the B. plicatilis obtained sufficient nutrition from Prorocentrum donghaiense to support net population increase. With exposure to 2.5×104 cells mL−1 of P. donghaiense, the number of B. plicatilis increased faster than it did when exposed to other four algal densities (5, 10, 15 and 20 ×104 cells mL−1), and the increase rate of B. plicatilis population (r) at this algal density was 0.104 ± 0.015 rd−1. Cell densities of P. donghaiense decreased due to the grazing of B. plicatilis. In contrast, Heterosigma akashiwo had an adverse effect on B. plicatilis population and its growth was largely unaffected by rotifer grazing. In this case, B. plicatilis population decreased and H. akashiwo grew at a rate similar to that of the control. 相似文献
6.
KANG Kyoung Ho 《中国海洋大学学报(英文版)》2009,8(3):265-269
Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To
estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the
cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds
of cryoprotectants (ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol) were employed at three concentrations
(1.0, 1.5 and 2.0 molL−1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants
after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide
at a concentration of 1.5 molL−1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival
rates of larvae frozen with 1.5 molL−1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23% ±6.22%
respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various
storage periods (p>0.05). 相似文献
7.
The seaweed Sargassum hemiphyllum is widely distributed throughout the coastal waters of Asia and has high commercial value.In recent years,its natural biomass has declined due to over-exploitation and environmental pollution.To seek for a feasible way to culture this seaweed efficiently,we designed a simple long-line system in a shrimp pond for the culture during winter,and the growth and nutritional composition of the seaweed were examined.Results show that the culture system was durable and flexible allowing S.hemiphyllum to grow vertically off the muddy bottom of the pond.Although the length of pondcultured S.hemiphyllum was inhibited by water depth,the weight-specific growth rate((1.65±0.17)%/d) was nearly three times higher than that of wild plants((0.62±0.19)%/d).The crude protein(6.92%±0.88%) and ash content(21.52%±0.07%) of the pond-cultured seaweed were significantly lower than those of the wild plants(9.38%±0.43% and 26.93%±0.07%,respectively);however,crude fat(1.01%±0.04%) was significantly higher than that of the wild plants(0.87%±0.02%).In addition,the nutritional composition of both pond-cultured and wild S.hemiphyllum was comparable to or even higher than those of other common seaweeds being used as food and/or aquaculture fodder.Future studies shall be focused on the impact of environmental parameters on its growth and nutritional composition. 相似文献
8.
Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10±0.09 g/L) and PHB production (1.11±0.13 g/L and 14.62%±2.25% of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH 4 + -N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65±0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03±0.18 g/L and 66.93%±1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77±0.58 g/L) and PHB production (6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield. 相似文献
9.
Effects of salinity, light and temperature on growth rates of two species of Gracilaria （Rhodophyta） 总被引：2，自引：0，他引：2
Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained
under the conditions of 32°C, 30 and 240 μmol/(m2·s) for G. lichenoides, and 24°C, 20 and 200 μmol/(m2·s) for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions
for the highest daily specific growth rates (% increase in wet weight) are determined to be 31.30°C, 32.10, and 287.23 μmol/(m2·s) for G. lichenoides (16.26%/d), and 25.38°C, 21.10, and 229.07 μmol/(m2·s) for G. tenuistipitata (14.83%/d), respectively.
Supported by the 908 Special Program (908-02-04-07), the National Basic Research Program of China (973 Program, No. 2006CB400608),
and K. C. Wong Magna Fund in Ningbo University 相似文献
10.
According to the known sequence of iron stress-induced gene (isiAB operon), we cloned its 1.5 kb fragment by PCR, and used this fragment as integration homologous fragment. After several steps of subcloning donor DNA into theisiAB fragment, a donor plasmid pZL which could be integrated into the chromosomal DNA ofSynechococcus sp. PCC7942 was constructed. In order to express the heterologous gene at a high level through the integration platform system, we constructed the donor DNA by the following steps. We cloned the strong promoter (240 bp) of heat shock genegroESL operon fromSynechococcus sp. PCC7942 by PCR. Then subcloned the multiple cloning sites (MCS),rbcS polyA into the downstream of thegroESL promoter. The kanamycin resistance gene, as the marker gene, was also subcloned into the donor DNA. Thus, in the donor plasmid pZL, the integration homologous fragment and several expression elements, such asgroESL promoter, MCS,rbcS polyA terminator and kanamycin resistance gene, were all included.
After naturally transformed and introduced the donor plasmid pZL intoSynechococcus sp. PCC7942, as in the pZL, the donor DNA sequence is flanked by two DNA fragments (0.4 kb and 0.7 kb) homologous to theisiAB fragment ofSynechococcus sp. PCC7942, the homologous DNA can recombine with the chromosomal DNA. After screening by kanamycin, the transformants which integrated the heterologous DNA were selected. The efficiency of transformation is about 1×10−6. By southern blot analysis, it was confirmed that the donor DNA had been integrated into the chromosomal DNA ofSynechococcus sp. PCC7942, located on the site of theisiAB gene, and can be replicated with the chromosomal DNA.
相似文献11.
ABU HENA M K 《中国海洋湖沼学报》2013,31(1):1-9
Shoot density, standing crop (above- and below-ground biomass) and habitat of salt marsh grass Porteresia coarctata were investigated along the coast of Bakkhali estuary, Cox’s Bazar, Bangladesh from January to December 2006. Shoot density of P. coarctata was influenced by season and was found to be higher (>2 500 shoots/m 2 ) in post-monsoon and minimal in monsoon season; plants were particularly active in vegetative propagation during pre-monsoon. Above-ground biomass was greater along the protected coast compared with the exposed one in this estuary. Below-ground biomass was higher (7.75-269.53 g DW/m 2 ) than that above ground (2.20-114.75 g DW/m 2 ). Standing crops of P. coarctata showed a negative relationship (R=-0.77; P<0.05) with sedimentation rate, while seasonal activity influenced sedimentation. The recorded sedimentation rate was lower (6.09 mg/(cm 2 ·d)) in pre-monsoon and highest (14.55 mg/(cm 2 ·d)) in monsoon season. The mean value of pore water salinity was higher (34.25±5.05) during post-monsoon and lowest (18.0±3.71) in monsoon season. The soil was sandy clay in this P. coarctata bed; it consisted of 86% sand, 13% clay and 1% silt. Soil organic matter dropped during the monsoon season (0.78%-0.67%) and was highest ((2.17±1.42)%-(2.3±1.47)%) during post-monsoon, probably owing to accumulation of decomposed peat on the marsh surface. The mean pore water NH 4 -N concentration ranged from 2.44±1.65 to 3.33±1.82 μg/L, with a minimum air temperature of 22.09°C in post-monsoon and a maximum of 31.16°C in pre-monsoon. Variations of physico-chemical parameters in the soil, water, and climate governed biological parameters of P. coarctata in the Bakkhali estuary, and were comparable with estuarine environments elsewhere. 相似文献
12.
Picoplankton distribution was investigated in different water masses of the East China Sea in November,2006 and February,2007.The autumn and winter cruises crossed three major water masses:the coastal water mass(CWM),the mixed water mass(MWM),which forms on the continental shelf,and the Kuroshio water mass(KWM).Picoplankton composition was resolved into four main groups by flow cytometry,namely Synechococcus,Prochlorococcus,picoeukaryotes,and heterotrophic bacteria.The average abundances of Synechococcus,picoeukaryotes,and heterotrophic bacteria were(0.63±10.88)×103,(1.61±1.16)×103,(3.39±1.27)×105 cells/mL in autumn and(6.45±8.60)×103,(3.23±2.63)×103,(3.76±1.37)×105 cells/mL in winter,respectively.Prochlorococcus was not found in the CWM and seldom observed in surface samples in either season.However,Prochlorococcus was observed in the MWM and KWM(approximately 10 3 cells/mL) in both autumn and winter.Synechococcus distribution varied considerably among water masses,with the highest levels in KWM and lowest levels in CWM.The depth-averaged integrated abundance of Synechococcus was approximately 5-fold higher in KWM than in CWM,which may be due primarily to water temperature.In the MWM,Synechococcus was resolved as two subgroups;the presence of both subgroups was more common in autumn.Picoeukaryote abundance varied less among water masses than Synechococcus,and heterotrophic bacteria depth-averaged integrated abundance exhibited the smallest seasonal variations with respect to water mass.Correlation analysis showed that relationships between picoplankton abundances and environmental factors(temperature,nutrients,and chlorophyll a) differed among the three water masses,suggesting that the three water masses have different effects on picoplankton distribution(particularly Synechococcus). 相似文献
13.
Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass of the rotifers in Yangcheng Lake was 2 894 ± 1 006 ind./L and 12.47 ± 10.28 mg/L, respectively. The highest densities were observed in the western portion of the lake in March, but the highest biomass occurred in inflowing creeks in September. Within a year of monitoring in Yangcheng Lake, 93 species were identified and the dominant species were found to be Polyarthra trigla, Brachionus angularis, Keratella cochlearis, Keratella valga, Brachionus calyciflorus, and Filinia major. Of the species recorded, 75 were pollution indicator species. Density and biomass exhibited significant positive correlations with water temperature (R = 0.209, P = 0.003; R = 0.446, P = 0.000), but the peak density showed two lags in response to chl a. According to the Jaccard similarity index (SJ), the greatest similarity among dominant species occurred between creeks and the eastern part of the lake. The annual average Shannon-Wiener diversity index H’, Margalef richness index D and Pielou evenness index J were 1.96 ± 0.34, 1.61 ± 0.50 and 0.77 ± 0.10, respectively. In all four areas of Yangcheng Lake, β- mesosaprobic species comprised the largest share of pollution indicator species. These data suggest that Yangcheng Lake is mesosaprobic. 相似文献
14.
Microcystis bloom, one of the most objectionable characteristics of eutrophication in tropical and subtropical waters, occurred in Donghu Lake (East lake) of Wuhan every summer from the 1970s up to 1984, but from 1985 up to now failed to occur there. The cause of its disappearance rema-ined in obscurity until recently. In situ enclosure experiments in the lake for three years showed that the stocking of the filter-feeding silver carp ( Hypophthalmichthys molitrix ) and big-head carp (Aristichthys nobilis ) played a decisive role in eliminating Microcystis bloom from the lake; but that recurrence of the bloom is possible under certain conditions. This paper presents the details and the results of enclosure ex-periments. The authors‘ analysis of fish biomass data obtained by echo-sounding and the fishery produc-tion of the lake over the years, revealed that the recurrence of Microcystis bloom can be prevented so long as the combined biomnss of silver carp and big-head carp remains at or exceeds 50 g per cubic meter of lakewater, as was the case in the lake‘s 1985 fish yield of 1015 t. 相似文献
15.
British Antarctic Survey (BAS) data collected near the Antarctic Peninsula area were used in this 1988/1989 study on the length
frequency of three Antarctic fish species (Pleuragramma antarcticum, Electrona antarctica and Protomyctophum bolini) showing that the standard length (SL) ofP. Antarcticum ranged from 26.0 mm to 161.0 mm, that the 26.0 mm to 54.0 mm SL juveniles could be considered to be the age group 1 fish,
and that the SL ofE. antarctica ranged from 39.4 mm to 89.7 mm for the males and 48.0 mm to 118.0 mm for the females, which may contain at least 6 age groups.
This is more than previously thought.P. Bolini had 29.2 mm to 60.2 mm SL and complicated age composition. 相似文献
16.
The ultrastructure of hemocytes ofPenaeus vannamei was examined by transmission electron microscope. Three types of hemocytes were identified: hyaline cell, small-granule cell
and large-granule cell. Hyaline cells are the smallest of the hemocytes, lack electron-dense granules, have average size of
11.11 μm (SE±0.50,n=10)×6.96μm (SE±0.55,n=10), comprise 25.8% (SE±2.87,n=4×10) of the hemocytes; small granule cells are the most abundant type of hemocytes, comprise 58.1% (SE±3.40,n=4×10) of the hemocytes, contain small electron—dense granules, have average size of 10.78 μm (SE±0.60,n=10)×8.63 μm (SE±0.44,n=10); and large-granule cells comprise 16.1% (SE±2.55,n=4×10) of the hemocytes, are filled with large electron dense granules, have average size of 12.51 μm (SE±0.63,n=10)×8.99μm (SE±0.71,n=10).
This research was supported by the National Climbing Project B, PDB6-6-3.
Contribution No. 3448 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
17.
Cao Ji-xiang 《中国海洋湖沼学报》1996,14(4):378-386
This study on the effect of different concentrations of germanium dioxide (GeO2) on the specific growth rate (SGR), pigment contents, protein content and amino acid composition ofSpirulina platensis showed that Ge was not the essential element of this alga; that GeO2 could speed up growth and raise protein content ofS. platensis, and could possibly influence the photosynthesis system. The concentration range of GeO2 beneficial to growth ofS. platensis is from 5–100mg/l. GeO2 is proposed to be utilized to remove contamination byChlorella spp. usually occurring in the cultivation ofSpirulina. 相似文献
18.
Phytoplanktonic desmids community in Donghu Lake, Wuhan,China 总被引：1，自引：0，他引：1
1 INTRODUCTION Donghu Lake (30o33′N, 114o23′E) is a shallow medium sized freshwater lake in Wuhan City, Hubei Province, China and about 5 kilometers away from the Changjiang (Yangtze) River. Before the 1960s, the water surface area was about 27.8 km2. The lake water was clear and the pH ranged from 6 to 7. The shallow area of the lake abounded with macrophytes. The phytoplanktonic and peripheral desmids were very abundant also in this lake. In the late 1960s, Donghu Lake with tota… 相似文献
19.
Flow cytometric determinations of the abundance distribution and community structure of picophytoplankton (i.e., Prochlorococcus spp., orange fluorescence Synechococcus spp. and picoeukaryotes) were used for samples taken from the Philippine Sea in the western tropical Pacific Ocean from September to October of 2004. A fluorescence probe was employed to detect Chlorophyll a (Chl a). Abundances of Prochlorococcus spp., orange fluorescence Synechococcus spp. and picoeukaryotes ranged from 0.1 to 58×103 cells ml?1, 0.38 to 17×102 cells ml?1 and 0.42 to 26×102 cells ml?1, respectively. Synechococcus spp. and picoeukaryotes co-occurred in relatively shallow water with the maximum abundance observed at 50 to 70 m depth, while Prochlorococcus spp. only occurred in the 70 to 200 m layer. Prochlorococcus spp. was the dominant picophytoplankton population in terms of abundance and biomass. The cell size and carbon biomass content were estimated for the three picophytoplankton groups. In addition, among the three groups of picophytoplankton, the relative contribution of red fluorescence to the total red fluorescence varied with depth. The fluorescence and light scatter properties of individual cells indicated that in the upper 100 m layer, picoeukaryotes were a major contributor to total red fluorescence, while at the depth below 100 m, Prochlorococcus spp. and Synechococcus spp. made an important contribution to the total red fluorescence. 相似文献
20.
The energy flow ofBranchiura sowerbyi was studied for the first time in China in a shallow macrophytic lake, Biandantang Lake, Hubei Province. The energy flow
was calculated from the measurement of flesh production (12.5241kJ/m2a), egestion (517.7302kJ/m2a), metabolism (38.3273 kJ/m2a), and excretion (4.3798kJ/m2a). The net growth efficiency of the species is about 22.7%, which accords well with the generally reported value for oligochaetes.
In addition, the relationship between starvation respiration (R, mgO2/ind·d), wet weight (Ww, mg) and temperature (T, °C) were also measured, with the regression function beingR=0.008Ww0.736 e0.050T.
Project supported by NSFC (30270278, 3960019), the foundation of the government of Hubei Province (No. 2000J109), and the
foundation of Ecological Station, CAS in the Institute of Hydrobiology. 相似文献