首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
Hepatic levels of Cu, Zn and metallothionein (MT) in perch, caught in a Cu/Zn gradient from a brassworks, reflected the water concentration of Cu (1·0–9·4 ppb) and Zn (0·56–59 ppb). Significant correlations were found between hepatic Cu and MT levels (r = 0·72), and between Zn and MT levels (r = 0·69). There was an increase of the amount of Cu and Zn in the cytosolic fraction of the liver with increased hepatic levels of the metals. When liver samples. from perch caught at the most contaminated location, were run on a gel filtration column (Sephadex G-75) 78% of the cytosolic Cu and 24% of the Zn in the cytosol eluted together with MT.  相似文献   

2.
Metallothionein has previously been shown to be regulated during the period of exogenous vitellogenesis (Olsson et al. (1987); Fish Physiol, Biochem., 3(1), 39–47). Following estradiol injection of rainbow trout it has been shown that hepatic metallothionein remains at basal levels until the vitellogenin mRNA levels begin to decline and Zn is released from high-molecular-weight proteins (Olsson et al. (1989); Biochem.J., 257, 555–559), In the present study the effects of estradiol treatment on the metal-inducibility of metallothionein have been investigated. Estradiol-treated fish that were injected with low doses of cadmium or zinc did not respond by induction of metallothionein.  相似文献   

3.
Metallothioneins and lysosomes are known to be involved in cellular detoxication and sequestration of certain metals1–3 and both have been identified in this role in elimination of copper from marine mussels (Mytilus edulis/galloprovincialis).3 Cadmium (Cd), however, has been shown to persist in the cells of the digestive gland for long periods with only minimal elimination. An experiment was designed to test the effects of Cd on the fragility of lysosomal membranes in the digestive cells as a measure of cellular injury,4,5 metallothionein content of the digestive gland and cadmium concentration in this organ. Phenanthrene was used also to destabilise lysosomal membranes6 in order to test if increased lysosomal fragility interfered with cadmium metabolism and detoxication. The results demonstrated that Cd induced metallothionein synthesis and that elimination of Cd was minimal after 28 days in clear seawater. Lysosomal fragility was initially increased but this effect was soon reversed, even with continued exposure to Cd. The lysosomal destabiliser, phenanthrene, did not appear to affect accumulation of Cd or levels of metallothionein.  相似文献   

4.
The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 μgCu l−1 on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP; thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P<0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P<0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within 1 day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P<0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.  相似文献   

5.
金属硫蛋白(Metallothionein,MT)是一类富含半胱氨酸的小分子蛋白质,参与机体重金属解毒和金属元素代谢等生理过程。本研究采用RACE技术,克隆获得了菲律宾蛤仔(Ruditapes philippinarum)金属硫蛋白(RpMT)的全长cDNA序列。RpMT的cDNA全长为570bp,编码75个氨基酸,包含15个MT所特有的Cys-Xn-Cys结构。采用实时荧光定量PCR技术,分析了两种壳色菲律宾蛤仔(白蛤和斑马蛤)RpMT基因在Cd2+暴露后的表达变化。结果发现:Cd2+急性和亚慢性暴露均可导致两种壳色蛤仔消化腺和鳃组织RpMT基因表达量的显著上调;暴露后两种壳色蛤仔鳃组织RpMT基因表达量的增加幅度均高于消化腺组织,且以白蛤鳃组织基因表达水平的上调幅度较高。上述结果表明,RpMT可能在菲律宾蛤仔抵御Cd2+胁迫过程中发挥了重要作用。  相似文献   

6.
Common dab were fed a casein based diet containing copper at a concentration of 1·4 mg copper kg−1 (dry weight) or a similar diet supplemented with 200 mg copper kg−1. Fish were sampled after 10 weeks and 20 weeks and hepatic and renal copper, zinc and metallothionein concentrations were measured. All hepatic cytosolic copper in test and control fish co-eluted from Sephadex G-75 with metallothionein, as did some of the cytosolic zinc. After 20 weeks there was three times as much of this metallothionein-bound copper in the livers of the test fish compared to the control fish, although metallothionein concentration was little elevated. On the basis of these results it is proposed that cytosolic liver copper concentration or heat stable liver copper concentration can be used as an indication of excess dietary copper in the dab Limanda limanda.  相似文献   

7.
Synthesis of metallothionein (MT) as a possible mechanism by which Fundulus heteroclitus adapts to heavy metal pollution has been investigated in relation to responses to methylmercuric chloride (meHg) and HgCl2(Hg2+) at different life stages.Clutches of embryos from unpolluted sites exhibit teratogenic responses to a standard dose of meHg or of Hg2+ which range from little or no effect (tolerant) to severely affected or almost complete inhibition of development (susceptible). At the time of hatching, untreated embryos from tolerant clutches had twice as much of a protein which co-migrates in SDS-PAGE gels with Fundulus MT as do those of susceptible clutches. This difference could be a mechanism for conveying tolerance to Hg2+ and also to meHg if demethylation occurs. However, eggs at the time of deposition had virtually no MT, suggesting that the protein is synthesized too late to provide tolerance in the early stages of development which are critical in the etiology of malformations. Treatment of embryos with non-teratogenic doses of either meHg or Hg2+ did not increase the amount of MT-like protein. This may indicate that the thionein gene is already fully active, and would also explain observations that Hg2+ pretreatment of embryos does not enhance tolerance to Hg2+ in the larval stage.MT, isolated from livers of Hg2+- or Cu2+-dosed or control adults, is associated with Cu and Zn but not with Hg, although it can bind Hg2+in vitro. Fundulus, like other fish, methylates much (ca. 75 %) of its Hg burden, and meHg is considered by some not to react with MT. Fundulus liver has its Hg burden distributed between homogenate sediment (25 %), the cytosol (60 %) and the remainder in lipid. The liver responds to Hg2+ treatment by increasing lipid content. This may allow sequestration of some lipid-soluble meHg. Of the cytosolic Hg, most is associated with larger proteins and some (ca. 25 %) is dialyzable.These data suggest that metallothionein is not a significant factor in Hg tolerance in Fundulus adults, and has a questionable role in embryonic and larval tolerance to Hg2+ and none to the more toxic meHg.  相似文献   

8.
Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass, Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish, Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure.  相似文献   

9.
The effects of injections of 17β-estradiol (E2) and Cd, on the distribution of Cd and the induction of metallothionein (MT) mRNA and vitellogenin mRNA was investigated. Bone and liver were the main organs accumulating Cd. However, E2 redirected the metal accumulation from the bone and liver to the gill, gut and muscle upon exposure to E2. Cd did not induce the hepatic MT mRNA levels in animals treated with E2. The VTG mRNA levels were also reduced following co-injection of E2 and Cd. However, the kidney responded to Cd exposure by upregulating MT mRNA even in the presence of E2 treatment. In the liver the reduced MT mRNA induction led to a redistribution of CD from MT to non-MT proteins. The toxicological significance of these alterations in Cd handling remains to be determined.  相似文献   

10.
An isolated fish hepatocyte culture system was developed as a models ystem to investigate the mechanisms of action of environmental contaminants. Hepatocytes were isolated from striped bass (Morone saxatilis) by an adaptation of the two stage perfusion technique of Seglen.1 The system was used to evaluate metal binding protein (MBP) induction in response to cadmium, a primary inducer of metallothionein (MT) in rat hepatocytes. Striped bass hepatocytes appeared to be refractory to the induction of MBP by cadmium, since there was no significant increase in the synthesis of MBP for any of the doses at any of the time points investigated. However, when a similar experiment was performed using rat hepatocytes there was induction of MBP that was related to both dose and time. These comparative experiments indicate that although there are similarities between the hepatocytes of the two species in regard to 35S incorporation into low molecular weight metal-binding proteins, there appear to be significant quantitative differences as well in regard to MBP kinetics. This in vitro model system could potentially be utilized to investigate the toxicological properties of other environmental contaminants.  相似文献   

11.
Mercury, cadmium, arsenic, and antimony were analyzed in cores sampled on the Azores-Iceland Ridge. High values of 780 g · kg–1 for Hg, 1.7 g · g–1 for Cd, 87 g · g–1 for As, and 8.1 g · g–1 for Sb occur in the rift valley and transform faults. These enrichments, strictly linked to the ridge, could not have an allochtonous origin. A local hydrogenous flux may explain this phenomenon. These metallic enrichments may be connected to a hydrothermal activity extended between 43° N and 47° N.  相似文献   

12.
An extensive trial to examine physiological and biochemical changes in perch (Perca fluviatilis) inhabiting coastal waters polluted by bleached kraft mill effluents has been carried out. The results from this investigation show profound effects of bleached kraft mill effluents on several fundamental biochemical and physiological functions on four different sampling occasions. Typical symptoms in perch from the polluted areas were reduced gonad growth, enlarged liver, and very strong induction of certain cytochrome P-450-dependent activities. Elevated content of ascorbic acid in liver tissue and strongly affected carbohydrate metabolism show that the effluent causes metabolic disorders. Marked effects on the white blood cell pattern indicate a suppressed immune defence. Alterations in the red blood cell status and the ion balance suggest an impaired gill function. This extensive symptom picture is strengthened by the relatively good agreement with the effects previously observed in fish exposed to bleached kraft mill effluents (BKME) in the laboratory.1–3  相似文献   

13.
The metal load into sediments and the change in the sedimentary environment of Osaka Bay in the Seto Inland Sea have been studied through geochemical analysis of core sediments, using both Pb-210 dating and a selective chemical leaching technique. Analytical results from a 6-m core of sediment show that copper and zinc pollution started in the late 1800's and the present enrichment ratios of copper and zinc, relative to background levels (20 mg kg–1 for Cu and 94 mg kg–1 for Zn), are 2.8 and 4.1, respectively. The present anthropogenic copper and zinc loads into Osaka Bay sediments, are 47 and 368 ton yr–1, while natural copper and zinc loads are 40 and 186 ton yr–1, respectively. Osaka Bay sediment at the present day is considered to be seriously polluted by zinc, now. The vertical profiles of copper and zinc in four successively separated fractions (10% acetic acid soluble fraction: F-HAC, 0.1M hydrochloric acid-soluble fraction: F-HCl, hydrogen peroxide-soluble fraction: F-H2O2 and hydrofluoric acid-soluble fraction: F-HF) from the core sediments indicate that enrichments of copper and zinc in the upper layer of the sediment are dependent on increases in the metal contents of the F-HAC, F-HCl and F-H2O2 fractions. Copper in F-HAC, and zinc in F-HAC and F-HCl, seem to be of anthropogenic origin.Results of sequential studies of the whole Seto Inland Sea can be summarized as follows: At the present time, the sedimentary loads of copper and zinc over the whole Seto Inland Sea area are 630 and 3,500 ton yr–1, respectively, while the natural and anthropogenic loads are 320 and 310 ton yr–1 for copper and 1,800 and 1,700 ton yr–1 for zinc, respectively.  相似文献   

14.
The abundance and vertical distribution pattern of a mysidMeterythrops microphthalma were investigated in the Japan Sea. Results from vertical hauls from 602–982 m depth to the surface around Yamato Rise in April 1987 indicated that the dominance (by biomass) ofM. microphthalma was third to fifth of major zooplankton taxa. Vertical distribution investigated at a single station in Toyama Bay in June, September and December 1986 showed that the most part of population of this mysid inhabited consistently below 250 m depth. No marked diurnal vertical migration was evident. Data on body composition and oxygen consumption rate ofM. microphthalma are presented. Water content of the body was 75.6–83.8% of wet weight, and ash was 11.4–20.4% of dry weight. Carbon, hydrogen and nitrogen were 37.9–47.5%, 6.2–7.4% and 9.4–10.1%, respectively, of dry weight. Oxygen consumption rates were 2.2–11.0µl O2 individual–1 hr–1 at 0.5°C, and were directly proportional to body mass. From the comparison with the published data on epipelagic and bathypelagic mysids it is revealed that both body nitrogen composition and oxygen consumption rate expressed as adjusted metabolic rate [AMR02,µl O2 (mg body N)–0.85 hr–1] ofM. microphthalma are intermediate between high epipelagic and low bathypelagic levels, indicating typical mesopelagic features.  相似文献   

15.
The effect of cadmium was determined on some oxygen-binding characteristics (viz. oxygen affinity, subunit cooperativity, Bohr-effect and temperature effect) of the erythrocruorin of the lugworm, Arenicola marina.Oxygen affinity and subunit cooperativity were measured directly in the blood of Arenicola, both in blood collected from specimens kept under experimental conditions and from specimens obtained from their natural habitat.In the pH range of 7·3–7·8, which closely resembles in vivo blood pH values, cadmium does not affect the oxygen affinity at low concentrations (10−8-10−5 ), and decreases the O2-affinity only slightly at higher concentrations (10−5-2·10−3 ). In the more alkaline pH range, the oxygen affinity increases considerably with increasing cadmium concentrations.Cadmium exerts a facilitating influence on the haem cooperativity at lowest concentrations (10−8-10−7 ) and a minor inhibiting influence at higher concentrations.The oxygen affinity, subunit cooperativity, the Bohr-effect and the temperature effect are pH dependent.The influence of cadmium on the magnitude of the Bohr-effect is pH-dependent.In vivo measurement of the oxygen affinity of Arenicola erythrocruorin gives evidence of a more pronounced inhibition of oxygen binding at increasing cadmium concentrations in the blood, compared with the effect on purified erythrocruorin solutions.  相似文献   

16.
The use of biomarkers such as cytochrome P4501A (CYP1A) or metallothionein (MT) for pollution monitoring is based on the assumption that an increased activity or concentration is primarily caused by exposure to contaminants. Previous studies have shown that the response of biomarkers may be affected by factors such as season, temperature, gender, nutritional status or size. The objective of the present study was to identify natural factors that affect the hepatic activity of CYP1A and hepatic concentration of MT in flounder (Platichthys flesus L.). Thirty flounder were sampled at each of two sites in the Hvaler archipelago, southern Norway, monthly through one year. A set of variables were recorded for each sampling and individual including water temperature, salinity, external and internal lesions, intestinal content, sex, size and organ weights. Hepatic CYP1A activity (EROD), MT and metal concentrations were determined for each individual flounder. The influence of environmental and endogenous factors on the response of these two biomarkers was assessed in multiple regression models. For both biomarkers, 50–60% of the total variability could be explained from factors directly related to season, gender and maturation. Season contributed significantly in the model for EROD, as did external lesions. Size, condition and diet did not contribute greatly when the above factors were included. The results confirm previous findings that season, gender and maturation must be taken into account in biomarker monitoring, but also indicate that other factors such as external lesions should be considered.  相似文献   

17.
We investigated the geographical variations in abundance and biomass of the major taxonomic groups of micro- and net-zooplankton along a transect through Ise Bay, central Japan, and neighboring Pacific Ocean in February 1995. The results were used to estimate their secondary and tertiary production rates and assess their trophic roles in this eutrophic embayment in winter. Ise Bay nourished a much higher biomass of both micro- and net-zooplankton (mean: 3.79 and 13.9 mg C m–3, respectively) than the offshore area (mean: 0.76 and 4.47 mg C m–3, respectively). In the bay, tintinnid ciliates, naked ciliates and copepod nauplii accounted for, on average, 69, 18 and 13% of the microzooplankton biomass, respectively. Of net-zooplankton biomass, copepods (i.e. Acartia, Calanus, Centropages, Microsetella and Paracalanus) formed the majority (mean: 63%). Average secondary production rates of micro- and net-zooplankton in the bay were 1.19 and 1.87 mg C m–3d–1 (or 23.1 and 36.4 mg C m–2d–1), respectively, and average tertiary production rate of net-zooplankton was 0.75 mg C m–3d–1 (or 14.6 mg C m–2d–1). Available data approximated average phytoplankton primary production rate as 1000 mg C m–2d–1 during our study period. The transfer efficiency from primary production to zooplankton secondary production was 6.0%, and the efficiency from secondary production to tertiary production was 25%. The amount of food required to support the zooplankton secondary production corresponded to 18% of the phytoplankton primary production or only 1.7% of the phytoplankton biomass, demonstrating that the grazing impact of herbivorous zooplankton was minor in Ise Bay in winter.  相似文献   

18.
  相似文献   

19.
Rainbow trout were exposed to 200 ppb cadmium in the water during four months at 5°C. The liver, kidney and gills were analyzed for cadmium, copper, zinc and metallothionein. Cadmium accumulated in all three organs and reached the highest concentration in the kidney. The zinc and copper concentrations were not altered during the experiment. The metallothionein concentration was significantly higher in liver of exposed fish than in control fish after three months. The kidneys reached significantly elevated levels of metallothionein in the exposed group after four months. These results demonstrate that metallothionein is induced by cadmium after exposure to the metal via water.  相似文献   

20.
Organisms have evolved a cellular response called stress protein response that increases their tolerance in adverse environmental conditions. Well known stress proteins that bind essential and toxic metals are metallothionein (MT). The scallop Mizuhopecten yessoensis is the most interesting organism because it is able to accumulate toxic cadmium in its digestive gland. However, in the tissue of the digestive gland of Mizuhopecten yessoensis MT (metallothioneins) have not been found. Eastern scallops, Mizuhopecten yessoensis, were collected from two locations ?? one clean and one polluted site. The concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were measured in the digestive gland. There was a significant increase in Cd concentrations in this studied tissue. We found that in the presence of cadmium Mizuhopecten yessoensis can induce high molecular proteins. The results of experiments have shown that Cd-binding ligands have a number of properties similar to MT: acetone and temperature stability; the ability to bind some metals, including Cd, Cu and Zn. Protein chromatography (FPLC, Superosa 12) from the digestive gland of scallop M. yessoensis has shown that cadmium is associated with high molecular weight Cd-binding proteins (72 kDa and 43 kDa). The major cadmium-binding protein 72 kDa is glycoprotein. In experiments we have demonstrated that Cd-binding proteins can be induced when there is cadmium exposure. The results of this study strongly suggest that the far eastern scallop Mizuhopecten yessoensis has a unique and well-developed system for the detoxification of heavy metals and it allows for biochemical systems to be maintained in a relatively stable manner in the presence of heavy metals.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号