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1.
Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with
muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized.
The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type I collagen, containing
α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations confirmed the existence of helical arrangements
in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts) of PSC were 29.4°C and 52.8°C, respectively. 相似文献
2.
The relationships between the neon flying squid, Ommastrephes bartrami, and the relative ocean environmental factors are analyzed. The environmental factors collected are sea surface temperature (SST), chlorophyll concentration (Chl-a) and sea surface height (SSH) from NASA, as well as the yields of neon flying squid in the North Pacific Ocean. The results show that the favorable temperature for neon flying squid living is 10℃-22℃ and the favorite temperature is between 15℃-17℃. The Chl-a concentration is 0.1-0.6 mg/m3. When Chl-a concentration changes to 0.12-0.14 mg/m3, the probability of forming fishing ground becomes very high. In most fishing grounds,the SSH is higher than the mean SSH. The generalized additive model (GAM) was applied to analyze the correlations between neon flying squid and ocean environmental factors. Every year, squids migrate northward from June to August and return southward during October-November, and the characteristics of the both migrations are very different. When squids migrate to the north, most relationships between the yields and SST are positive. The relationships are negative when squids move to southward. The relationships between the yields and Chl-a concentrations are negative from June to October, and insignificant in November. There is no obvious correlation between the catches of squid and longitude, but good with latitude. 相似文献
3.
<正>柔鱼(Ommastrephes bartramii)广泛分布在北太平洋,20世纪70年代初首先由日本鱿钓船开发,我国大陆于1993年开始利用该资源,1994年进行较大规模地商业性生产。目前北太平洋鱿钓渔业已成为我国远洋渔业的支柱[1]。据估计,历史上北太平洋柔 相似文献
4.
2004年北太平洋柔鱼钓产量分析及作业渔场与表温的关系 总被引：1，自引：0，他引：1
根据2004年5~11月我国鱿钓船在北太平洋生产数据,结合表温资料,按经纬度1°×1°的格式,利用Marineexplorer 4.0软件作图进行柔鱼钓产量及渔场与表温的关系分析。结果表明,5~7月在160°E以东海域作业,产量较低;8~10月在150°~160°E海域作业,为生产作业的产量高峰期,占总产量的62.5%;11月在150°E以西海域作业,产量也较低。在150°E以西海域CPUE最高,150°~160°E中部海域次之,160°E以东海域最低。作业渔场的适宜表温呈现出季节性变化。各月适宜表温分别为:5月12~14℃;6月15~16℃;7月14~16℃;8月18~19℃;9月16~17℃;10月15~16℃;11月12~13℃。 相似文献
5.
Rheological and Structural Properties of Sea Cucumber Stichopus japonicus During Heat Treatment 总被引：6，自引：0，他引：6
GAO Xin XUE Dongmei ZHANG Zhaohui XU Jiachao XUE Changhu 《中国海洋大学学报(英文版)》2005,4(3):244-247
Changes in tissue structure, rheological properties and water content of raw and heated sea cucumber meat were studied. Sea cucumber Stichopus japonicus was heated at 25℃, 70℃ and 100℃ water for 5 min. The structural changes were observed using a light microscope and the rheological parameters （rupture strength, adhesive strength and deformation） determined using a texture meter. Microscopic photograph revealed that the structural change of heated meat was greater than that of raw meat. The rupture strength, adhesive strength and deformation of raw meat were smaller than those of the heated meat. Meanwhile, rheological parameters showed positive correlation with heating temperature. These changes are mainly caused by thermal denaturation and gelatinization of collagen during heating. These changes were also evidenced in observations using a light microscope and differential scanning calorimetry. 相似文献
6.
应用间接酶联免疫吸附试验(ELISA)技术测定不同水温下鳜经嗜水气单胞菌GYK1株全菌灭活疫苗浸泡免疫后皮肤黏液中抗体,以研究抗体动态变化,揭示温度对抗体产生的影响。结果表明:27.0~29.8℃水温(下称"较高温")下,鳜皮肤黏膜抗体滴度于第7天达到峰值211,而水温介于19.8~23.9℃(下称"较低温")时,抗体滴度于第11天达到峰值26,说明温度较高时,抗体形成快,峰值出现时间早,并且抗体滴度明显升高。另取不同温度下经上述疫苗浸泡免疫的鳜皮肤黏液,采用硫酸铵盐析、离心、浓缩等生物化学技术粗提鳜皮肤黏液中的免疫球蛋白,SDS-PAGE检测发现,较高水温下,高渗免疫组(加10 mg/mL食盐)鳜皮肤黏液蛋白中目标条带(72×103和29×103)深度较其他3个实验组(同温度下全菌组、较低水温下高渗及全菌组)有所增加,ELISA检测该组抗体效价为211。 相似文献
7.
《中国海洋大学学报(英文版)》2015,(6)
We applied solution-based ICP-MS method to quantify the trace-elemental signatures in statoliths of jumbo flying squid, Dosidius gigas, which were collected from the waters off northern and central Chile during the scientific surveys carried out by Chinese squid jigging vessels in 2007 and 2008. The age and spawning date of the squid were back-calculated based on daily increments in statoliths. Eight elemental ratios(Sr/Ca, Ba/Ca, Mg/Ca, Mn/Ca, Na/Ca, Fe/Ca, Cu/Ca and Zn/Ca) were analyzed. It was found that Sr is the second most abundant element next to Ca, followed by Na, Fe, Mg, Zn, Cu, Ba and Mn. There was no significant relationship between element/Ca and sea surface temperature(SST) and sea surface salinity(SSS), although weak negative or positive tendency was found. MANOVA analysis showed that multivariate elemental signatures did not differ among the cohorts spawned in spring, autumn and winter, and no significant difference was found between the northern and central sampling locations. Classification results showed that all individuals of each spawned cohorts were correctly classified. This study demonstrates that the elemental signatures in D. gigas statoliths are potentially a useful tool to improve our understanding of its population structure and habitat environment. 相似文献
8.
The relationships between the neon flying squid, Ommastrephes bartrami, and the relative ocean environmental factors are analyzed. The environmental factors collected are sea surface temperature
(SST), chlorophyll concentration (Chl-a) and sea surface height (SSH) from NASA, as well as the yields of neon flying squid in the North Pacific Ocean. The results
show that the favorable temperature for neon flying squid living is 10°C–22°C and the favorite temperature is between 15°C–17°C.
The Chl-a concentration is 0.1–0.6 mg/m3. When Chl-a concentration changes to 0.12–0.14 mg/m3, the probability of forming fishing ground becomes very high. In most fishing grounds,
the SSH is higher than the mean SSH. The generalized additive model (GAM) was applied to analyze the correlations between
neon flying squid and ocean environmental factors. Every year, squids migrate northward from June to August and return southward
during October–November, and the characteristics of the both migrations are very different. When squids migrate to the north,
most relationships between the yields and SST are positive. The relationships are negative when squids move to southward.
The relationships between the yields and Chl-a concentrations are negative from June to October, and insignificant in November. There is no obvious correlation between
the catches of squid and longitude, but good with latitude.
Supported by the National High Technology Research and Development Program of China (863 Program, No. 2003AA607030); National
Key Technology Research and Development Program (No. 2006BAD09A05) 相似文献
9.
Low molecular weight peptide (LMWP) was prepared from clam Paphia undulate and its antiaging effect on D-galactose-induced acute aging in rats, aged Kunming mice, ultraviolet-exposed rats, and thermally injured rats was investigated. P. undulate flesh was homogenized and digested using papain under optimal conditions, then subjected to Sephadex G-25 chromatography to isolate the LMWP. Administration of LMWP significantly reversed D-galactose-induced oxidative stress by increasing the activities of glutathione peroxidase (GPx) and catalase (CAT), and by decreasing the level of malondialdehyde (MDA). This process was accompanied by increased collagen synthesis. The LMWP prevented photoaging and promoted dermis recovery and remission of elastic fiber hyperplasia. Furthermore, treatment with the LMWP helped to regenerate elastic fibers and the collagen network, increased superoxide dismutase (SOD) in the serum and significantly decreased MDA. Thermal scald-induced inflammation and edema were also relieved by the LWMP, while wound healing in skin was promoted. These results suggest that the LMWP from P. undulate could serve as a new antiaging substance in cosmetics. 相似文献
10.
1 INTRODUCTION Long-term exposure of the skin to ultraviolet (UV) radiation results in degenerative processes involved in photoaging and photocarcinogenesis (Differy, 1987). As UVC is absorbed entirely by the stratospheric ozone layer and does not reach the earth抯 surface, sunlight is composed of UVA (320 nm 400 nm) and UVB (290 nm 320 nm). Human skin is exposed constantly to both UVA and UVB radiation, it is, therefore, important to protect the skin against UVA and UVB-induce… 相似文献
11.
智利外海茎柔鱼耳石生长特性的性别差异研究 总被引：1，自引：0，他引：1
根据2007年1~6月和2008年1~5月我国鱿钓船在智利外海调查期间采集的茎柔鱼耳石样本,测量了503枚耳石外部形态参数(雌性348枚,雄性155枚),结合耳石日轮,对耳石生长特性的性别差异进行了研究.结果表明,耳石总长(TSL)、耳石半径(SR)、吻侧区长(RLL)、翼长(WL)及最大宽度(MW)可作为茎柔鱼耳石形态的表征参数.雌雄个体间耳石的生长在5个形态参数上均存在显著性差异,雌性个体 TSL 与日龄间关系最适为指数函数, SR 和 RLL 为幂函数, MW 和 WL 则为线性函数;雄性个体的 TSL、SR 和 RLL 与日龄间的关系最适为幂函数, MW 和 WL 为线性函数.雌性个体 TSL、SR、MW、RLL、WL 的相对生长率和SR、RLL 绝对生长率均随着日龄的增加而先增加后减小, TSL、MW 绝对生长率随着日龄的增加而增加,WL绝对生长率则随着日龄增加而呈现波动趋势;雄性个体 TSL、SR 相对生长率和 MW、RLL、WL 绝对生长率随着日龄增加先增加后减小,而 MW、RLL 和 WL 相对生长率和 TSL、SR 绝对生长率则随日龄增加呈现波动趋势.研究认为,茎柔鱼耳石生长存在性别差异,通常雌性生长较雄性快 相似文献
12.
ZHUANG Yongliang LI Bafang ZHAO Xue College of Food Science Engineering Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2009,8(2)
Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superox-ide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scav-enge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries. 相似文献
13.
The eastern fall cohort of the neon flying squid, Ommastrephes bartramii, has been commercially exploited by the Chinese squid jigging fleet in the central North Pacific Ocean since the late 1990s. To understand and identify their optimal habitat, we have developed a habitat suitability index (HSI) model using two potential important environmental variables - sea surface temperature (SST) and sea surface height anomaly (SSHA) - and fishery data from the main fishing ground (165°-180°E) during June and July of 1999-2003. A geometric mean model (GMM), minimum model (MM) and arithmetic weighted model (AWM) with different weights were compared and the best HSI model was selected using Akaike’s information criterion (AIC). The performance of the developed HSI model was evaluated using fishery data for 2004. This study suggests that the highest catch per unit effort (CPUE) and fishing effort are closely related to SST and SSHA. The best SST- and SSHA-based suitability index (SI) regression models were SISST-based = 0.7SIeffort-SST + 0.3 SICPUE-SST, and SISSHA-based = 0.5SIeffort-SSHA + 0.5SICPUE-SSHA, respectively, showing that fishing effort is more important than CPUE in the estimation of SI. The best HSI model was the AWM, defined as HSI=0.3SISST-based+ 0.7SISSHA-based, indicating that SSHA is more important than SST in estimating the HSI of squid. In 2004, monthly HSI values greater than 0.6 coincided with the distribution of productive fishing ground and high CPUE in June and July, suggesting that the models perform well. The proposed model provides an important tool in our efforts to develop forecasting capacity of squid spatial dynamics. 相似文献
14.
Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH
was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical
and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl
radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native
antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results
showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding
capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries. 相似文献
15.
温度对奥尼罗非鱼仔稚鱼生长、饲料利用和消化酶活力的影响 总被引：1，自引：0，他引：1
研究了温度22、25、28、31和34℃下奥尼罗非鱼(Oreochromis niloticus×O.aureus)仔稚鱼生长、饲料利用和消化酶活力。结果表明:1)不同温度对奥尼罗非鱼仔稚鱼生长有显著影响(P<0.05),温度为31℃时,仔稚鱼生长速度和绝对增重率最高,分别为0.08cm/d和0.035g/d;温度为22℃时最低,仅0.06cm/d和0.020g/d。2)饲料系数在28℃时最低,仅1.90;22和34℃时较高,达2.14和2.17,各组差异显著(P<0.05)。3)在仔稚鱼发育和存活率上,当温度在22～34℃时,温度越高,仔稚鱼发育越好;各组仔稚鱼的存活率介于93.7%～94.7%,差异不显著(P>0.05)。4)在消化酶活力上,胃蛋白酶、胰蛋白酶和淀粉酶活力随温度升高而升高,均在34℃时活力最高;脂肪酶活力在31℃时达到最高,22℃时活力最低。 相似文献
16.
A comparison of fishery biology of jumbo flying squid, Dosidicus gigas outside three Exclusive Economic Zones in the Eastern Pacific Ocean 总被引：1，自引：0，他引：1
Although many studies on the fishery biology of jumbo flying squid, Dosidicus gigas, have been conducted in the coastal areas within Exclusive Economic Zones (EEZs) of various countries due to its commercial and ecological importance, limited biological information is available from waters outside these EEZs. In this paper, we examined D. gigas fishery biology from waters outside Chilean, Peruvian and Costa Rican EEZs, based on the fishery data collected by Chinese jigging vessels during 2006 to 2010. The dominant mantle lengths of D. gigas were 350–450 mm, 250–400 mm and 250–350 mm outside Chilean, Peruvian and Costa Rican EEZs, respectively. Size structure analysis show that a medium-sized group existed mostly in the waters outside the Chilean and Peruvian EEZs, whereas a small-sized group occurred mainly in the waters outside the Costa Rican EEZ. The longevity of the squid outside the Costa Rican EEZ was less than 10 months, while most of those outside Chilean and Peruvian EEZs were about 1–1.5 years and very few large individuals were 1.5–2 years old. A higher percentage of mature individuals existed outside Costa Rican EEZ implying the region as a potential spawning ground, while lower proportions of mature squid outside the Peruvian and Chilean EEZs indicated that spawning may be occurring outside our study area. Spatial differences in sizes at maturity of the squid are thought to be result from different environmental factors especially different temperature and nutrition among the three areas. Stomach-content analysis showed that cannibalism was important in the diet of D. gigas. Stress generated by jigging may increase the incidence of cannibalism. 相似文献
17.
描述了智利外海南极褶柔鱼Todarodes filippovae的形态特征,并参考前人研究资料,确定了其分类地位,同时对其作了初步分析。鉴定认为:该种系柔鱼科、褶柔鱼亚科、褶柔鱼属的南极褶柔鱼;其最北分布可到27°57′S,比原先文献报道的35°S偏北;渔获物的调查期间的胴长为157～366mm,体重为65～960g,性腺成熟度以Ⅰ、Ⅱ期为主,占总样本的71%。同时,还建立了胴长与体重、胴长与角质额喙长的关系式。 相似文献
18.
温度对虎斑乌贼受精卵孵化及幼体存活的影响 总被引：1，自引：0，他引：1
研究海水温度对虎斑乌贼(Sepia pharaonis)受精卵孵化及幼体存活的影响。结果表明:虎斑乌贼胚胎发育的最低和最高临界水温为20℃和32℃,孵化的最适温度范围为24~30℃。水温[t(℃)]与孵化率[H(%)]间的函数关系可表示为:H=-5.333+2.583 t(R2=0.247,F=7.213,P<0.01);虎斑乌贼幼体对温度的耐受力最低和最高临界温度分别为12和32℃,存活最适温度范围为22~28℃。温度[T(℃)]和处理时间[t(h)]及其交互作用均对虎斑乌贼的存活率[S(%)]有显著的影响(P<0.01),其函数关系可表示:S=60.526+1664 T-0.588 t(R2=0.572,F=39.407,P<0.01)。 相似文献
19.
GAO Hongwei） ）* MAO Mao） LIANG Chengzhu） LIN Chao） XIANG Jianhai）） Institute of Oceanology Chinese Academy of Sciences Qingdao P. R. China ） College of Food Science Engineering Ocean University of China P. R. China ） Sh ong Entry-Exit Inspection Quarantine Bureau of P. R. China P. R. China 《中国海洋大学学报(英文版)》2009,8(1):95-99
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he... 相似文献
20.
The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature. 相似文献