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1.
Discharge of heavy metals from metal processing industries is known to have adverse effects on the environment. Biosorption
of heavy metals by metabolically inactive biomass of microbial organisms is an innovative and alternative technology for removal
of these pollutants from aqueous solution. The search of marine actinobacteria with potential heavy metal biosorption ability
resulted in the identification of a novel alkalophilic Streptomyces VITSVK5 species. The biosorption property of Streptomyces VITSVK5 spp. was investigated by absorbing heavy metals Cadmium (Cd) and Lead (Pb). Physiochemical characteristics and trace
metal concentration analysis of the backwater showed the concentrations of different metals were lead 13±2.1 μg L−1, cadmium 3.1±0.3μg L−1, zinc 8.4±2.6μg L−1 and copper 0.3±0.1μg L−1, whereas mercury was well below the detection limit. The effect of pH and biomass dosage on removal efficiency of heavy metal
ions was also investigated. The optimum pH for maximal biosorption was 4.0 for Cd (II) and 5.0 for Pb (II) with 41% and 84%
biosorption respectively. The biosorbent dosage was optimized as 3 g L-1 for both the trace metals. Fourier transform infrared
absorption spectrum results indicated the chemical interactions of hydrogen atoms in carboxyl (-COOH), hydroxyl (-CHOH) and
amine (-NH2) groups of biomass with the metal ions. This could be mainly involved in the biosorption of Cd (II) and Pb (II) onto Streptomyces VITSVK5 spp. The results of our study revealed Streptomyces metabolites could be used to develop a biosorbent for adsorbing metal ions from aqueous environments. 相似文献
2.
ZHANG Jimin ) LIU Shuang ) SUN Xue ) YANG Guanpin ) ZHANG Xuecheng ) * GAO Zhenhui ) ) North China Sea Monitoring Center SOA Fushun Road Qingdao P..R.China) College of Marine Life Sciences Ocean University of China Qingdao P..R.China 《中国海洋大学学报(英文版)》2003,2(1)
1 Introduction Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans . Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo… 相似文献
3.
Genetic Analysis of Four Populations of Redlip Mullet(Chelon haematocheilus) Collected in China Seas
MENG Wei) GAO Tianxiang) * and ZHENG Bing) ) Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P. R. China ) Shandong Ocean Exploration Center Qingdao P.R.China 《中国海洋大学学报(英文版)》2007,6(1):72-79
1 Introduction The redlip mullet, (Chelon haematocheilus) (Tem-minck et Schlegel, 1845), belongs to the family Mugili-dae. It is mainly distributed in the coastal areas of Korea, Japan and China (Wang et al., 2000). Because of the good adaptation to envir… 相似文献
4.
The GRV 024516 and GRV 024517 meteorite samples collected from Grove Montains,Antactica are ureilite and H5 ordinary chondrite,respectively.Based on the study of mineralogy-petrology,the cosmic-ray exposure ages and gas retention ages of these two meteorites were determinated and calculated.Their cosmic-ray exposure ages are 33.3 Ma,51.7 Ma,and gas retention ages are 1936.8 Ma and 3720 Ma,respectively.The ureilite contains diamond,graphite and amorphous C,which are mainly carrier of noble gases indicating obviously shock metamorphism effects,which induced 40Ar partial loss.The H5 chondrite indicates thermal metamorphism of parent body,its gas retention age fall the range between 3220 Ma and 4510 Ma of the least shocked H5 chondrites 相似文献
5.
Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE 总被引：1，自引：0，他引：1
WEN Ruobing) SUI Zhenghong) * ZHANG Xuecheng) ZHANG Shuang) and QIN Song) ) Laboratory of Marine Genetics Breeding College of Marine Life Sciences Ocean University of China Qingdao P. R. China ) Institute of Oceanology Chinese Academy of Sciences Qingdao P.R. China 《中国海洋大学学报(英文版)》2007,6(4):373-377
Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value. 相似文献
6.
1 INTRODUCTIONTherateoffoodconsumptionoffishfedadlibitumisregardedasthemaximumrateoffoodconsumption(Cmax) (Woottonetal.,1 980 ) .SeveralfactorsthatinfluenceCmaxincludingbodyweight (Liuetal.,1 998) ,watertemperature (Liuetal.,1 998) ,dis solvedoxygen (Vivekanandan ,1 977) ,salinity (ZanuyandCarrillo ,1 985 ) ,andphotoperiod (Grossetal.,1 965 ) ,werereported .Bodysizeandwatertemperaturewereregardedastwoofthemostimportantfactors,andhadreceivedmostattention (Elliott,1 979;Woottonetal.,1 … 相似文献
7.
Matthias Kuhle Geographie 《山地科学学报》2006,(3)
3 The Highest Former Trim-lines and Gla-cier Thicknesses in the Khumbu Hima-laya: Mt. Everest, Lhotse- and Cho Oyu- S- and W-slopes The glaciation history of the Khumbu Himal, i.e., of the Himalaya- and especially of the Mt. Ev-erest-S-slope can only be reconstructed and under-stood if one also has a detailed knowledge of the N-slope of the area, because the High- to Late Gla-cial ice stream networks on the Tibetan side of the Mt. Everest-massif here, too, were connected to the Himal… 相似文献
8.
The species of Epithemia ocellata (Ehr.) Kütz. from Northeastern China were observed by scanning electron microscopy (SEM). The studies on silica valve formation in different stages were made. At the early stage, a Y-shaped raphe sternum was found. Areolae were made from virgae and vimines. In immature valves, the canal formation before the raphe fissure is visible. The raphe system, virgae and vimines (areolae) might be the early siliceous elements of the valve during morphogenesis. The formations of virgae and vimines are similar to that of Gephyria media W. Arnott. Rows of silica papillae formed gradually on the face of the valve. After the areolae were covered with silica and form rows of papillae, the outer valve surface could become mature. Details of the internal costae were observed in mature valves. 相似文献
9.
Microcystins(MCs) are cyclic hepatotoxic peptides produced by the bloom-forming cyanobacterium Microcystis and present a public health hazard to humans and livestock. The removal of MCs from contaminated water with powdered activated carbon(PAC) has been employed as a simple and economic treatment strategy. In this study, PAC-Fe(Ⅲ) was prepared and utilized for the fast and efficient removal of MCs from water. PAC-Fe(Ⅲ) exhibited superior microcystin-LR(MC-LR) removal capacity and efficiency compared to the unmodified PAC. The MC-LR removal efficiency of PAC-Fe(Ⅲ) increased with decreasing p H within the pH range of 4.3 to 9.6. PAC-Fe(Ⅲ) could be reused for 3 times by methanol elution while the MC-LR removal efficiency was still over 70 percent. The removal efficiency was positively correlated to the ionic strength of water and negatively correlated to alkalinity. Natural organic matter(NOM) such as humic acid(HA) and salicylic acid(SA) generated low interference with MC-LR adsorption by PAC-Fe(Ⅲ). The complexation reaction between Fe~(3+) in PAC-Fe(Ⅲ) and the functional groups of MCLR was suggested as the key mechanism of MC-LR removal by PAC-Fe(Ⅲ). The results suggest that Femodified PAC is a promising material for the treatment of MC-contaminated waters. 相似文献
10.
ZHENG Xiaodong ) * ZHAO Jianmin ) XIAO Shu ) WANG Rucai ) WANG Shidang ) ZHOU Weiwu ) ) The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P..R.China ) Wendeng Fishery Technical Extension Station Weihai P..R.China 《中国海洋大学学报(英文版)》2004,3(1)
1 Introduction ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof… 相似文献
11.
This paper reports the concentrations of 137 Cs, hexachlorocyclohexane(HCH), dichlorodiphenyltrichloroethanes(DDT) and its main degradation products, δ13C, and organic carbon in pond sediments(0-210 cm, sectioned by 2-20 cm interval) and surface soils(the 0-3 cm horizon) collected in 2010 from Chenjia catchment, which is located in Yanting county in the hilly central Sichuan of China. α-, δ-, and γ-HCH, DDT, and DDD were not detected throughout the sediment profile. Trace concentrations of δ-HCH(0.89-29.31 ng g-1) and p,p′-DDE(1.85-6.02 ng g-1) were detected only in top 40 cm sediment. The 137 Cs fallout peak in 1963(corresponding to the 55-60 cm depth), the sedimentary signature left by the last year of HCH use in 1989(an additional indicator at 20-25 cm), and the obvious original channel bed prior to the construction of the pond in 1956 were used as temporal markers to estimate changes in average sedimentation rate between different periods due to changes in land use. Continuous, marked decrease in average sedimentation rate(i.e., 3.79, 1.35 and 1.07 cm year-1 in 1956-1963, 1963-1989, and 1989-2010, respectively)over time was observed, probably due to the reforestation, abandoning of steep sloping farmland for afforestation and natural re-vegetation(implementation of the Grain for Green Program), and the conversion of part of gently sloping farmland terraces to orchard land since the 1980 s, especially since the 1990 s. This was corroborated by the observed decrease(more negative) in δ13C of sediment towards the surface, which indicates increased relative contribution of eroded soil particles coming from slopes with increased tree cover in sediment source area. Combined use of 137 Cs, δ-HCH, and δ13C record in sediments has been demonstrated to be a powerful approach to reconstruction of response in sedimentation rate to historical land use changes. 相似文献
12.
1 INTRODUCTION The first published records of genus Batra-chospermum Roth in China are from Jao (1941), when eleven taxa were reported. Since then, Shi, Hu and Kumano (1993), Shi (1994a, 1994b), Hua and Shi (1996), Xie et al. (1999, 2003), Xie and Shi (2003) have described in more detail the species from China. To date, 23 taxa of Batrachospermum Roth in China have been reported, of which, B. yunnanense, B. nothocladoideum and B. transito-rium are new species of this genus, and are… 相似文献
13.
XU Jiachao GAO Xin ZHANG Zhaohui LI Zhaojie HUO Lihua and XUE Changhu Department of Food Science Engineering Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2006,(2)
Fucoidan is such a polysaccharide that its hydroxies are easy to combine with lanthanons ion (CeIV) to form complex. This work obtained the complexes of three fucoidan oligosaccharides with different molecular weights F1(>5000), F2 (1000-5000) and F3(<1000) by hydrolyzing Oligosaccharide collagen with sulfuric acid. It is found that the fucoidan oligosaccharide F3 can form complex with more CeIV than F1 and F2. Hydrolyzing collagen with the complex was carried out to produce amino acid and peptides. All the three fucoidan oligosaccharide complexes with CeIV( F1, F2, F3) can catalyze by the artificial hydrolytic enzyme, and the activity of the complex of F3 is the highest. 相似文献
14.
1IntroductionThe Tibet plateau consists of terranes which were accreted in a southward-younging succession from Late Permian to Mid-Eocene (Dewey etal., 1988; Mattern etal., 1998; Mattern and Schneider, 2000). The Songpan-Ganzi terrane was accreted to the Kunlun during Late Permian along Kunlun-Qin-ling suture. The Qiangtang block was added onto the Songpan-Ganzi terrane at the Jinsha suture dur-ing Late Triassic-Early Jurassic. The Lhasa block became attached to the Qia… 相似文献
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16.
Shuangtaizi estuarine wetland along the Bohai Sea coast,the biggest bulrush wetland in the world,has been listed in ‘The Record of Important International Wetland Conservation District’. Taking the year of 2 000 as an example,the minimum,the most suitable and the maximum ecological water requirement of Shuangtaizi estuarine wetland are calculated in this paper based on both ecological theory and Geological Information System technology. In addition,the remote sensing technique is adopted in the data acquisi... 相似文献
17.
《中国海洋大学学报(英文版)》2005,(4)
Editor-in-Chief: WEN Shengchang Academician of Chinese Academy of Science, Ocean University of China, P.R.C Deputy Editors-in-Chief: LI Huajun Mar, Eng. Col., OUC, P. R. C SONG Weibo Fisheries Col., OUC, P. R. C LIU Qinyu Phys. & Environ. Oceanogr. Col., OUC, P. R. C WANG Lin Environ. Sci. & Eng. Col., OUC, P. R. C Executive Deputy Editor-in-Chief: PENG Yuan Editorial Office of Journal of Ocean University of China, P.R.C 相似文献
18.
A new kinetic spectrophotometric method is developed for the measurement of manganese (Ⅱ) in water. The method is based on the catalytic effect of manganese (Ⅱ) with the oxidation of weak acid brilliant blue dye (RAWL) by KIO4 using the Nitrilo triacetic acid (NTA) as an activation reagent. The optimum conditions obtained are 40mgL-1 RAWL, 1×10-4molL-1 KIO4, 2×10-4molL-1 Nitrilo triacetic acid (NTA), pH = 5.8, the reaction time of 3.00 min and the temperature of 20.0 ℃. Under the optimum con-ditions, the proposed method allows the measurement of manganese (Ⅱ) in a range of 0-50.0ngmL-1 and with a detection limit of down to 0.158 ng mL-1. The recovery efficiency in measuring the standard manganese (Ⅱ) solution is in a range of 98.5%-102%, and the RSD is in a range of 0.76%-1.25%. The new method has been successfully applied to the measurement of manganese (Ⅱ) in both fresh water and seawater samples with satisfying results. Moreover, few cations and anions interfere with the measurement of man-ganese (Ⅱ). Compared with other kinetic catalytic methods and instrumental methods, the proposed method shows fairly good selec-tivity and sensitivity, low cost, cheapness, low detection limit and rapidity. It can be applied on boats easily. 相似文献
19.
本文以黄土岭滑坡为例，分析了滑坡产生的机理、稳定性等，提出了防治对策及加强对山前残坡积粘土膨胀性等测试的必要性。 相似文献
20.
Jos E Celis Winfred Espejo Ricardo Barra Daniel Gonzalez-Acua Francisca Gonzalez Solange Jara 《极地研究(英文版)》2015,(1)
In recent decades, polar regions of the planet have witnessed an increase in human presence. Antarctica is considered one of the most pristine regions of the world, but it could be affected by pollutio... 相似文献