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1.
Flexibility is an important adaptive attribute of the feeding periodicity of grazing mayfly larvae because most natural environments offer a wide variation in local predation risk in terms of space, time or predator species. In this study any changes of diel feeding periodicity and consumption rates of Baetis rhodani (Ephemeroptera) were analysed in response to different densities of benthivorous fish (Barbatula barbatula, Gobio gobio) by quantifying gut fullness using the fluorescence of algal pigments. Laboratory experiments with the grazer species B. rhodani were conducted by using different concentrations of chemical fish cues. In order to assess the transferability of the results to a larger scale, the experimental results were compared with field observations in two second order streams using different densities of freely foraging benthivorous fish. During the presence of chemical fish cues in the laboratory experiments the feeding periodicity of the B. rhodani larvae were mostly diurnal while in the absence of fish chemicals nocturnal feeding was observed. The same patterns could be detected in the field during the experiments with the different fish densities. These findings indicate that the larvae were able to assess variations in the predation risk and to alter their feeding habits by making flexible behavioural adjustments. The results from the laboratory experiments further suggested that the behavioural response is controlled by fish density. Behavioural changes were observed for medium and high concentrations of the fish cues but not for a very low concentration. In the field however, the mere presence of fish seemed to be sufficient to induce the observed behavioural shifts. Although the presence of benthivorous fish seemed to cause a lower consumption rate of the B. rhodani larvae in the field, such a reduction could not be found in the laboratory experiments. A conclusion from this study is that the identification of behavioural modifications is an essential component needed for a better understanding of complex trophic interactions in benthic communities. Accurate evaluation and detailed observation of direct and indirect effects cannot be made without the consideration of such behavioural mechanisms.  相似文献   

2.
24 h of in vivo exposure to 40 and 80 = LC50 of the synthetic pyrethroid cypermethrin significantly reduced the acetylcholinesterase, cytochrome oxidase and lactic dehydrogenase activity in the nervous tissue of L. acuminata. Succinic dehydrogenase activity increased significantly after the same treatment. Fifteen minutes in vitro exposure to different doses of cypermethrin also significantly inhibited cytochrome oxidase and lactic dehydrogenase activity. However, acetylcholinesterase and succinic dehydrogenase activities remain unaltered. It is concluded that the cypermethrin on the one hand causes repititive discharges at the nerve terminal and on the other hand blocks the electron transport chain.  相似文献   

3.
In the batch experiment with daily exchange of medium the acute toxicity of Ag from AgNO3 to Channa punctatus, Lebistes reticulatus and Puntius sophore is determined over 12 to 96 h exposure. In general, the sensitivity of species increases in the order mentioned. The LC50, 96h are 18.89 μg/l Ag for Channa punctatus, 7.55 μg/l Ag for Puntius sophore and 6.44 μg/l for Lebistes reticulatus. For 12 and 48 h exposure the values in the same order of species are: 62 and 30, 44 and 14 as well as 57 and 16 μg/l Ag.  相似文献   

4.
A static renewal toxicity test was conducted to evaluate the response of the copepod Acartia tonsa to bis(tributyltin) oxide (TBTO), the active compound of recently developed antifouling paints. Copepods were individually placed in test tubes containing 4 ml of 0.3, 0.5, 1.0, 1.7 or 3.0 μg l?1 TBTO with acetone and seawater. Thirty copepods were used for each treatment and control condition and all solutions were renewed daily. The 96-h LC50 was 1.0 μg l?1 with a 95% confidence interval between 0.8 and 1.2 μg l?1 TBTO. The 144-h EC50 was 0.4 μg l?1 TBTO which approaches concentrations recently measured in San Diego Bay. Some copepods exposed to 0.3 μg l?1 TBTO became moribund after six days.  相似文献   

5.
The 96h LC50 of the water soluble fraction (WSF) of natural gas condensate from the Sable Is., N.S. area to Fundulus heteroclitus was between 4 and 5 μg · ml−1 naphthalene equivalents (initial concentration). Behavioural responses in the fish to sub-lethal concentrations of WSF were similar to those recorded for crude and fuel oils. Induction of the hepatic mixed function oxidase enzyme enthoxyresorufin O-de-ethylase (EROD) in sexually immature fish occurred at WSF concentrations below the LC50, but during the prespawning period no induction occurred. EROD activity in unexposed fish showed seasonal variation consistent with differences in its inducibility.  相似文献   

6.
Yezin Dam is a man-made reservoir located close to Yezin village in Myanmar. Its water is used for irrigation, domestic purposes and as drinking water for many urban communities in the watershed area. In recent years, increased pollution due to the concurrent development around the dam has led to water quality deterioration. No detailed study on the distribution of cyanobacteria and toxin production has been conducted so far. In order to provide insight into the extent of cyanobacteria and cyanotoxins in the dam, water samples were collected once in January 2014 for the isolation of cyanobacterial strains and eight times between March 2017 and June 2018 for the investigation of physical, chemical and biological parameters. A total of 99 phytoplankton taxa belonging to 50 genera were recorded from Yezin Dam. Microscopic examination showed that a Dolichospermum sp. was the dominant cyanobacterium followed by small numbers of Microcystis, and Raphidiopsis raciborskii in all samples throughout the sampling period. 15 isolated cyanobacterial strains were classified morphologically and phylogenetically as Dolichospermum smithii, R. raciborskii and Microcystis and tested for microcystins (MCs), cylindrospermopsins (CYNs), saxitoxins (STXs) and anatoxins (ATXs) by liquid chromatography-tandem mass spectrometry (LC–MS/MS) and enzyme-linked immunosorbent assay (ELISA). The toxin analysis of all isolated Dolichospermum strains by ELISA and LC–MS did not indicate the presence of ATXs, STXs, CYNs nor MCs. Four of the five isolated Raphidiopsis strains produced CYN and deoxyCYN. One of the isolated Microcystis strains (AB2017/08) from Yezin Dam produced 22 MC congeners. Concentrations of 0.12 μg L−1 CYNs and 0.34 μg L−1 MCs were also found in an environmental sample from Yezin Dam by ELISA. The potential therefore exists for the use of untreated water from Yezin Dam to cause harmful effects on humans, domestic and wild animals.  相似文献   

7.
Exposure of Channa striatus over 24 h to 40% and 80% of the LC50;24h of the three pyrethroids cypermethrin, permethrin, and fenvalerate did not cause any significant changes in the levels of total protein and free amino acids in the liver and muscle tissues of the fish. After 96 h exposure, the protein amino acid levels are altered significantly.  相似文献   

8.
The toxicity of Na-pentachlorophenate to Viviparus bengalensis is investigated by batch tests with daily exchange of medium in the temperature range between 19 °C (February), 27 °C (April), 32 °C (June) and 30 °C (August) at times of exposure between 12 and 96 h. In the range of higher concentrations, pronounced abnormalities in behaviour by secreting mucus and discharging eggs and embryos occur. The LC50 are lowest for all times of exposure at 27 °C, whereas the times of survival with a given concentration are highest at 30 °C. From the LC50, 96h, 27°C = 66 μg/l results a safe concentration of only 47 μg/l Na-pentachlorophenate for Viviparus bengalensis.  相似文献   

9.
Crassostrea gigas spat were cultured for 8 weeks in the presence of a mixed algal diet in experimental tanks containing bis (tributyl tin) oxide (TBTO) alone, TBTO and a marine sediment, or a marine sediment alone. Growth measurements taken on a weekly basis showed enhanced weight gain in oysters exposed to nominal 50 and 100 mg l?1 concentrations of marine sediment alone. Spat in low level TBTO treatments (0.15 μg l?1) grew less well than the controls and showed pronounced thickening of the upper shell valve. High levels of TBTO (1.6 μg l?1) severely inhibited oyster growth. Tissue concentrations of TBT compounds were measured at the end of the experiment. Bio-concentration factors of 3000- and 10000-fold were demonstrated for the high and low TBTO treatments respectively.  相似文献   

10.
We examined the life history and secondary production of four Ephemeroptera species (Baetis alpinus Pictet, 1843–1845, Baetis rhodani Pictet, 1843–1845, Rhithrogena carpatoalpina Klonowska, Olechowska, Sartoriet & Weichselbaumer, 1987 and Habroleptoides confusa Sartori & Jacob, 1986) in a temperature stable cold spring stream at Prosiek valley (Cho?ské vrchy Mts., West Carpathians, Slovakia). We have found asynchronous bivoltine life cycle for the most abundant species B. alpinus with growth rate positively correlated to photoperiod length. R. carpatoalpina have shown unusual asynchronous univoltine life cycle and B. rhodani have shown uncommonly low abundance in mayfly community. Total secondary production of mayfly community was very low, reaching 1654.8 mg DW m?2 y?1. We suggest that the observed asynchrony in growth could be related to the lack of temperature control.  相似文献   

11.
Human pharmaceuticals, like the lipid lowering agent gemfibrozil and the non-steroidal anti-inflammatory drug diclofenac are causing environmental concern. In this study, the marine mussel (Mytilus spp.) was exposed by injection to environmentally relevant and elevated (1 μg/L and 1000 μg/L) concentrations of both compounds and biomarker expression was observed. Gemfibrozil exposure induced biomarkers of stress (glutathione S-transferase and metallothionein) at both concentrations 24 h and 96 h after exposure, respectively. Biomarkers of damage (lipid peroxidation (LPO) and DNA damage) were significantly affected, as well as the biomarker for reproduction, alkali-labile phosphate assay, indicating the potential oxidative stress and endocrine disrupting effect of gemfibrozil. Diclofenac significantly induced LPO after 96 h indicating tissue damage. Additionally standard toxicity tests using the marine species Vibrio fischeri, Skeletonema costatum and Tisbe battagliai showed differences in sensitivity to both drugs in the mg/L range. Results indicate a suite of tests should be used to give accurate information for regulation.  相似文献   

12.
An investigation on the levels of mercury contamination in one species of the Cyanophyta, 14 species of the Rhodophyta, five species of the Phaeophyta and six species of the Chlorophyta from Penang waters indicated fairly low levels of bioaccumulated total mercury content. The bioaccumulated values ranged from below the detectable level to 0.35 μg g?1 except for Padina sp. 1 which demonstrated an amount equivalent to 1.025 μg g?1.These results indicate that the level of mercury contamination in the marine environment of Penang island falls within an acceptable range from the viewpoint of algal biodeposition.  相似文献   

13.
This study investigated the effects of contamination by copper and freshwater on the behaviour of the estuarine gastropod Polinices sordidus. The burying response was inhibited by both pollutants. In the case of copper, it was suggested that this response after 30 min could reflect toxicity after 96 h, i.e. the 96 h LC50 and the 30 min ED50 were very similar. P. sordidus responded to freshwater at concentrations well below the 96 h LC50, suggesting that this species can detect sublethal concentrations of freshwater. Thus, for salinity, the 30 min ED50 is not a reliable indicator of the 96 h LC50. A linear decline in crawling activity was found in the presence of increasing concentrations of both pollutants. These results for both aspects of behaviour are discussed in the light of results reported earlier for a marine species Polinices incei.  相似文献   

14.
From December 1997 to December 1998, benthic algal biomass and the input of allochthonous coarse particulate organic matter (CPOM) were investigated in bi-weekly intervals at two sampling stations (shaded and sunny) of the Weidlingbach, a fourth order sandstone stream in the Wienerwald. A total of 41 benthic algal taxa was collected, belonging to the groups Cyanobacteria (4 taxa), Bacillariophyceae (33), Rhodophyta (1), Chrysophyceae (1) and Chlorophyta (2). At the shaded site, periphyton dry mass ranged from 13 g m−2 in April to 440 g m−2 in August (annual mean = 93 g m−2), at the sunny site from 3 g m−2 in May to 512 g m−2 in late fall (annual mean = 70 g m−2). Based on the algal carotenoid pattern, Bacillariophyceae were most abundant in fall and winter while Chlorophyceae dominated during summer. Mean annual standing stock of chlorophyll-a was 8.65 μg cm−2 at the shaded station and 7.53 μg cm−2 at the sunny site. Annual allochthonous CPOM input rates ranged from 382 to 665 g dry mass m−2 for aerial input and from 1006 to 1062 g DM m−1 of stream length for lateral input. Lateral input rates were influenced by the bank inclination; the temporal distribution of aerial input showed an autumnal maximum (61-65% of the total). Direct CPOM input was significantly highest (P<0.05) during the period of defoliation from October to November. In the course of this period, 61.1% (shaded) to 64.9% (sunny) of the annual CPOM dropped into the brook, yielding daily input rates of 6.6 to 4.0 g DM m−2. From December to September, daily direct input rates decreased to 0.84 g DM m−2 (shaded) and 0.44 g DM m−2 (sunny). At both sites, retention capacity was high; 70% of marked leaves released along transects were retained by coarse sediment particles within 40 m from the starting point. The annual mean of periphyton dry mass made up 52% of CPOM standing stock at the shaded site and 39% at the sunny site.  相似文献   

15.
We studied the distribution of cyanotoxins and potential producers, as well as the variability of microcystin to biomass parameters (chlorophyll-a; MC/Chl-a; and biovolume; MC/BV) in 12 drinking water reservoirs of the world’s largest reservoir system, the Volga-Kama-Don cascade (European part of Russia) during the summers of 2016 and 2018. MC concentrations varied from below 0.1 μg L−1 in June up to 16.4 μg L−1 in August and exceeded 1 μg L-1 in 25 % of the samples. This MC variability was associated to changes in the abundance of widespread bloom formers such as Microcystis spp., Dolichospermum spp. and Planktothrix agardhii. Ratios of MC/Chl-a and MC/BVcyano ranged up to 0.88 μg μg−1 and 4.5 μg mm−³, respectively. Together with microcystin profiles MC/BVcyano ratios characterized cyanobacterial populations along the reservoir cascade and they indicated a potential toxin hazard better than MC/Chl-a. The neurotoxin anatoxin-a was observed only in the most southern and hypereutrophic Tsimlyansk Reservoir (maximum 0.01 μg L−1). Toxin gene analysis revealed that MC mostly originated from Microcystis and Dolichospermum. During their co-existence up to 14 MC congeners co-occurred. Cuspidothrix issatschenkoi cf. Raphidiopsis mediterranea was identified as possible neurotoxin producers.  相似文献   

16.
Competitive solid phase enzyme immunoassays using polyclonal antibodies were developed for the detection of the phenoxycaboxylic acids MCPB [4-(4-chloro-2-methylphenoxy)butyric acid], Mecoprop [2-(4-chloro-2-methylphenoxy)propionic acid], and 2,4-D [(2,4-dichloro-phenoxy)acetic acid] in drinking water and ground water. The carrier protein for the immunization was bovine serum albumin, horseradish peroxidase conjugates were employed as enzyme tracer. For the three antisera, the optimization of detection limits and test sensitivities was our first consideration. For the mecoprop and 2,4-D antisera, the strongest influences were the pH value and the ionic strength, as much as the use of enzyme tracers with lower affinities. The MCPB antiserum reacted with 2,4-DB[4-(2,4-dichlorophenoxy)butyric acid] with equal specificity, either could be detected at 0.02 μg/L (80% B/B0), middle of the test (50 B/B0) lying at 0.1 μg/L. The detection limit with mecoprop antiserum was optimized to 0.02 μg/L, the prescribed limit for drinking water of 0.1 μg/L ling at 60 % B/B0. The strongest cross-reactivity was found for mecoprop methyl ester. MCPB. 2,4-DB, and dichloroprop have crossreactivities of 50%, 6.7%, and 6.3%, respectively. The 2,4-D antiserum reacts less sensitively with 2,4-D, the detection limit being 0.4 μg/L. The 2,4-D isooctyl ester and 2,4-D methyl ester demonstrate as cross-reacting compounds high cross-reactivites of 3630% and 2230%. The cross-reactions of the compounds 2,4-DB, MCPB, and MCPA [(4-chloro-2-methylphenoxy)acetic acid] lie at 52%, 69%, and 41%. 100 ground water tests were spiked within laboratory. All positive samples were correctly identified. Falsely negative results did not appear.  相似文献   

17.
Drastic changes in the composition and physiognomy of riparian vegetation, such as the conversion of grassland to forest, are expected to alter interactions among light availability, primary producers and herbivores. Our aim was to examine in laboratory the influence of a ubiquitous grazer on periphyton grown in a grassland unshaded stream (reference) vs. periphyton from a nearby pine afforested stream. Besides, we evaluated how the community responds to the removal of grazing. Given that grassland streams are exposed to higher light intensity and grazers are more abundant compared to afforested streams, we proposed that if biofilm grown in the afforested stream are dominated by grazing-vulnerable algal species, grazing pressure by Helicopsyche sp. should be stronger. In addition, if biofilm from the afforested stream has low quality or is less abundant as food for consumers, the effects of Helicopsyche sp. may be stronger or weaker depending on their feeding decisions. Helicopsyche sp. caused a decrease in richness and diversity in periphyton grown in the grassland stream and its net grazing effect on chlorophyll a (Chl a) was higher. Algal community composition from grassland stream was strongly changed after grazing, with a decrease in the proportion of overstory algae. In contrast, algal community structure of periphyton from the afforested stream was neither affected by grazing nor by grazing exclusion. Helicopsyche sp. produced significant changes in a short time in structural attributes of algal communities, mainly in periphyton from the grassland stream suggesting that herbivory, as a functional factor, is diminished following afforestation.  相似文献   

18.
Acidic atmospheric deposition has adversely affected aquatic ecosystems globally. As emissions and deposition of sulfur (S) and nitrogen (N) have declined in recent decades across North America and Europe, ecosystem recovery is evident in many surface waters. However, persistent chronic and episodic acidification remain important concerns in vulnerable regions. We evaluated acidification in 269 headwater streams during 2010–2012 along the Appalachian Trail (AT) that transits several ecoregions and is located downwind of high levels of S and N emission sources. Discharge was estimated by matching sampled streams to those of a nearby gaged stream and assuming equivalent daily mean flow percentiles. Charge balance acid-neutralizing capacity (ANC) values were adjusted to the 15th (Q15) and 85th flow percentiles (Q85) by applying the ANC/discharge slope among sample pairs collected at each stream. A site-based approach was applied to streams sampled twice or more and a second regression-based approach to streams sampled once to estimate episodic acidification magnitudes as the ANC difference from Q15 to Q85. Streams with ANC <0 μeq/L doubled from 16% to 32% as discharge increased from Q15 to Q85 according to the site-based approach. The proportion of streams with ANC <0 μeq/L at low flow and high flow decreased from north to south. Base cation dilution explained the greatest amount of episodic acidification among streams and variation in sulfate (SO42−) concentrations was a secondary explanatory variable. Episodic SO42− patterns varied geographically with dilution dominant in northern streams underlain by soils developed in glacial sediment and increased concentrations dominant in southern streams with older, highly weathered soils. Episodic acidification increased as low-flow ANC increased, exceeding 90 μeq/L in 25% of streams. Episodic increases in ANC were the dominant pattern in streams with low-flow ANC values <30 μeq/L. Chronic and episodic acidification remain an ecological concern among AT streams. The approach developed here could be applied to estimate the magnitude and extent of chronic and episodic acidification in other regions recovering from decreasing levels of atmospheric S and N deposition.  相似文献   

19.
We tested the effect of two algal diets (Chlorella vulgaris at 1 · 106 cells mL?1and Scenedesmus acutus at 0.57 · 106 cells mL?1) offered alone or in combination (50 % each, 75 % or 25 % of either alga), at a total dry weight of 14.2 μg mL?1on the life table demography of two rotifer species, Brachionus calyciflorus and Brachionus patulus. Data on fecundity indicated that, for any given algal food type or combination of food types, B. calyciflorus had a higher rate of offspring production than B. patulus. Regardless of the rotifer species, the average lifespan varied from 4 to 6 days. The gross and net reproductive rates were higher for B. calyciflorus than for B. patulus. These values ranged from 45 to 71 offspring female?1 lifespan?1 and 15 to 24 offspring female?1 lifespan?1, respectively, for B. calyciflorus. Generation times (around 4…5 days) were similar for both rotifer species in most of the treatments. Whatever the algal food combination, the rate of population increase (d?1) of B. patulus was less than half (i.e. 45 %) of that for B. calyciflorus. The observed differences in the life history variables of Brachionus probably explain species‐specific adaptations to the changing phytoplankton composition occurring seasonally in natural waterbodies.  相似文献   

20.
Rain and surface water samples from Southern Germany were investigated from 1991 to 1995 for terbuthylazine and one of its major metabolites, deethylterbuthylazine. The concentrations observed were compared to the concentrations found for atrazine and deethylatrazine in the same water samples. Concentrations ranged from < 0.02 μg/L to 0.7 μg/L for terbuthylazine and from < 0.02 μg/L to 0.6 μg/L for deethylterbuthylazine, compared to concentrations of < 0.02 μg/L to 3 μg/L and < 0.02 μg/L to 0.5 μg/L for atrazine and deethylatrazine, respectively. The ratios of metabolite concentrations to parent compound concentrations were calculated for deethylterbuthylazine to terbuthylazine (DTR) and deethylatrazine to atrazine (DAR). In rain water, DTR of 0.8…3.0 and DAR of 0.3… 1.9 were determined with mean values of 0.9… 1.7 for DTR and 0.6…0.9 for DAR in the different years. The ratios increased during summer periods. The highest ratios were observed in samples from forest stands, showing that degradation of the herbicide has occurred during transport between the source and the sampling site. The DTR in rain water were about 50… 100% higher than the DAR. This indicates a higher degradation rate of terbuthylazine during atmospheric transport. In surface water, DTR of 0.3… 1.2 with mean values of 0.5…0.8 and DAR of 0.2…2.2 with mean values of 0.2… 1.3 were observed. The ratios increased from June to September.  相似文献   

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