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1.
The present study investigated conditions for inducing mito-gynogenetic (endomitosis) diploids by hydrostatic pressure in
the large yellow croaker Pseudosciaena crocea. In haploid control groups, the development of eggs was activated with ultraviolet radiated semen. All fry presented typical
haploid syndrome in the haploid control groups, and were verified as haploids using cytometry. After hydrostatic pressure
treatment, morphologically normal fry reappeared at different frequencies according to the intensity and time of pressure
shock. Fry with normal appearance in the pressure treated groups were verified as gynogenetic double haploids (GDHs), containing
only one allele from the female parent at all four diagnostic microsatellite loci. For a fixed duration of 3 min, the optimal
intensity of blocking the first mitosis was determined to be 40 Mpa, which was similar to that of blocking the second meiosis.
There was a “window” of starting time, from 36.1 min to 38.1 min post-insemination at 25.0±1.0°C, within which the production
of GDHs was not significantly different. Maximum production of morphologically normal fries, 9.36%±2.97% of developed eggs,
was found when the eggs were shocked with hydrostatic pressure at 40 Mpa for 3 min, starting from 38.1 min post insemination
at 25.0±1.0°C. 相似文献
2.
The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 min at 55 min after fertilization (a.f.), while for tetraploid it was 60 Mpa for 5 min at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage. 相似文献
3.
在多级滑坡的渐进破坏过程中, 滑带不同部位的屈服程度和破坏模式不同, 强度参数也不同。在强降雨条件下, 坡表产生的张拉裂缝充水, 会产生静水压力。当前广泛应用的传递系数法对滑带不同位置取同一强度参数, 也尚未考虑到静水压力作用。为此提出了一种考虑静水压力作用和滑带不同部位强度参数差异的改进传递系数法, 对降雨引起的西安市柳西村南部的牛角沟滑坡进行了计算。结果表明: 与不考虑静水压力和滑带不同部位强度参数差异的计算方法比, 改进传递系数法计算的抗滑力相对较小, 剩余下滑力计算结果相对较大, 各级滑坡稳定性系数分别减小了约33.26%、17.92%、24.95和16.94%;而改进前的稳定性系数偏高, 可能会导致支挡工程的安全储备不足。本研究提出的改进传递系数法可为多级滑坡处置提供更安全的参考。 相似文献
4.
Alaska pollock is an important protein source which is extensively used in the food industry. Pollock protein isolates(PPI) with significantly enriched protein contents could be prepared using isoelectric solubilization/precipitation(ISP) processing; however, the functional properties of this process is limited by the large amount of water-insoluble proteins. In this study, we investigated the influence of high hydrostatic pressure(HHP) treatment on the solubility and structural changes of PPI. PPI obtained using ISP is treated with hydrostatic pressures of 200, 300, 400, and 500 MPa for up to 15 min, and the HHP-treated samples were observed to exhibit significantly improved solubilities. Further biochemical assays reveal that the continuous HHP treatments reduce the contents of free sulfhydryl groups and promote the formation of macromolecules with better water solubilities, which may induce the solubility improvements of the HHP-treated PPI. Our results indicate that HHP can be utilized to effectively prepare highly water-soluble Alaska pollock protein in food processing. 相似文献
5.
河口捕获平均全长２．５ｃｍ的天然花鲈苗种，分二阶段进行培育。第一阶段是流水淡化驯养：包括淡化、暂养和驯饵；第二阶段是分级培育，每隔４～６ｄ分筛分级一次，直至４～６ｃｍ的鱼种。试验总成活率是５５％。 相似文献
6.
ARTIFICIAL PROPAGATION AND BREEDING OF MARINE FISH IN CHINA 总被引：3，自引：0，他引：3
Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of artificial fry can meet the needs of both marine cage culture and pond culture for most species. Experimental results obtained by scientists have been put into use in actual production.Fish fry production has entered a period of sustainable development. So far, at least 44 species (21 families) of marine fish have been successfully bred in China. The artificial fry number of large yellow croaker ( Pseudosciaena crocea) exceeded 300 million in 1999. The species whose artificial fry numbers have each surpassed 10 million annually are red drum ( Sciaenops ocellatus ), Japanese seabass ( Lateolabrax japonicus), cuneate drum (Nibea miichthioides ), spring spawning red seabream (Pagrosomus major ) and threebanded sweethp ( Plectorhynchus cinctus ). Millions of artificial fry are bred annually in the species of black porgy ( Sparus macrocephalus ), Russell‘ s snapper ( Lutjanus russeUi ), javelin gnmt ( Pomadasys hasta ), miiuy croaker ( Miichthys miiuy) and skewband gnmt ( Hapalogenys nitens ). The fish in the family Sciaenidae are the main species in artificial propagation and breeding. Some problems and prospects on marine fish culture and stock enhancement are also discussed and some proposals for sustainable development are put forward in this article. 相似文献
7.
全长5.8—6.4mm 具卵黄和油球的仔鱼开口摄食轮虫和无节幼体。稚鱼选择枝角类为主。幼鱼对桡足类的选择明显地高于枝角类,对轮虫和无节幼体则无选择性。早期幼鱼还摄食摇蚊幼虫,虾,草鱼苗和本种小鱼。26日龄幼鱼平均日增长1.18mm,日增重22.31mg。数理统计分析确定,全长与日龄呈直线关系。体重与日龄,全长对体重均呈幂函数关系。 相似文献
8.
研究了云斑尖塘鳢(Oxyeleotris marmorata)幼鱼的摄食特性。结果表明:在幼鱼阶段,云斑尖塘鳢的摄食方式为主动掠食;个体全长10mm的日摄食量约为体重的11.66%,40mm的日摄食量约为体重的51.04%,69mm的日摄食量约为体重的55.30%;日摄食量与幼鱼全长呈正相关关系:A=11.3577+0.7664L(r=0.876);云斑尖塘鳢幼鱼的适口饵料鱼全长为其全长的25%～50%,幼鱼摄食15～17h后可完全消化饵料鱼;云斑尖塘鳢幼鱼昼夜摄食节律明显,一昼夜出现一个摄食高峰,高峰时段为19:00-01:00。 相似文献
9.
WEN Yanmao WEI Xiange SHU Tingfei ZHOU Jingfeng YU Guanghui LI Feng HUANG Yanyun 《中国地理科学(英文版)》2007,17(4):370-375
In order to approach the characteristics of nitrogen and phosphorus pollution caused by cage culture and the balance of nitrogen and phosphorus during the process of cage culture, a monitoring was conducted in Daya Bay of Guangdong Province, China from April 2002 to Jane 2003. The results show that the concentrations of total nitrogen (TN) in the waters at the sites with five and ten years of cage culture history are 1.8 and 2.3 times of that at control site respectively. Ammonium (NH3-N) is the main form of nitrogen in spring while nitrate ( NO3 -) in winter. The concentrations of TN, total dissolved nitrogen (TDN) and dissolved organic nitrogen (DON) are highest in autumn. The concentration of phosphorus increases with the increasing of the culturing time, among which phosphate ( PO 34-) increases most obviously. The concentrations of total phosphorus (TP) and total dissolved phosphorus (TDP) are highest in autumn. The nitrogen and phosphorus are accumulated significantly in the sediment of cage culture area. The model of N balance in the cage culture area: bait (70.62%) fry (0.28%) input by tide (14.8%) release from sediment (14.3%) = harvest of adult fish (12.07%) deposition into sediment (28.75%) output by tide (56.18%) others (3.00%). The model of P balance: bait (83.11%) fry (0.17%) input by tide (12.23%) release from sediment (4.49%) = harvest of adult fish (8.43%) deposition into sediment (48.59%) output by tide (41.94%) others (1.04%). In one fish growth year, the contents of nitrogen and phosphorus in harvest of adult fish are only 17.0% and 10.1% of the contents of nitrogen and phosphorus in fish bait and fry, wherein 83% of nitrogen and more than 89% of phosphorus in fish bait became marine pollutants. 相似文献
10.
the effects of recombinant eel growth hormone (reGH), methyltestosterone (MT) and L-thyroxine (T4) on the growth of red sea
bream,Pagrosomus major, were investigated. Administration of reGH to fry by immersion at 2 mg/1 for 2 h every 5 days resulted in significant increase
in both weight and length, but the condition factor (CF) diminished relative to that of similarly treated controls over the
37 day treatment period. Immersion in 0.1 mg/l T4 also resulted in significant increase in both weight and length and higher
survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high
mortality. In the second study, injection of 2 μg reGH/(g·wk) caused a significant increase in the specific growth rate (SGR)
of test red sea bream fingerlings relative to that of the controls during the 4—week treatment period and maintained the increasing
trend over the post-treatment period (weeks 4–6). Injection of MT at a dosage of 1μg/(g·wk) resulted in a significant increase
in SGR during the 4—week treatment period. Intramuscular injection of 0.1 μg T4/(g·wk) also resulted in a significant increase
in weight. Injection of high-doses of MT and T4 inhibited growth and resulted in darkening skin, bulging eyes and thinning
body. In muscle chemical composition, the treated groups had no significant differences compared to the controls, but the
high-dose MT and T4 groups showed significant increases in lipid content. Potential practical methods for hormone application
in fish culture are discussed. 相似文献
11.
????GPS?????????????(PWV)??????????????????????????SA????????????????GPS???????????????????GPS????????????????????????θ???????????????ù??????????????????????????С??100 m???????????????????μ????????????Saastamoinen?????????????????????????????????IGS???BJFS??KUNM??LHAZ??TWTF??????????????????????????????????GPS PWV????????????????????RS PWV?????ж???????????????????????????θ???????????????????GPS PWV??RS PWV??????????????1~3 mm?????????????????????????????????GPS????PWV?? 相似文献
12.
四年来，在研究了大口黑鲈生物学特性的基础上，吸收了国内外的养殖经验，结合实际确立了在水泥池和池塘中繁殖鱼苗、培育鱼种和池塘主养、配养食用鱼的养殖技术。 相似文献
13.
《中国海洋湖沼学报》2020,(2)
Trimethylamine N-oxide(TMAO) is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial strains.To assess the impact of TMAO on marine microorganisms,especially those dwelling in the deep-sea environment,we analyzed the bacterial community structure of deep-sea sediments after incubated under different conditions.Enrichments at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure(HHP).We found that pressure was the primary factor that determines the bacterial community.Meanwhile,in total,238 bacterial strains were isolated from the enrichments,including 112 strains a ffiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains a ffiliated to 11 genera of 2 phyla from the Mariana Trench.Treatment of HHP reduced both abundance and diversity of isolates,while the presence of TMAO mainly af fected the diversity of isolates obtained.In addition,certain genera were isolated only when TMAO was supplemented.Taken together,we demonstrated that pressure primarily defines the bacterial community and culturable bacterial isolates.Furthermore,we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure condition.The results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment. 相似文献
14.
《中国海洋大学学报(英文版)》2018,(6)
Artificial gynogenesis is of great research value in fish genetics and breeding technology. However, existing studies did not explain the mechanism of some interesting phenomena. Severe developmental defects in gynogenetic haploids can lead to death during hatching. After diploidization of chromosomes, gynogenetic diploids may dispense from the remarkable malformation and restore the viability, although the development time is longer and the survival rate is lower compared with normal diploids. The aim of this study was to reveal key mechanism in haploid syndrome of Japanese flounder, a commercially important marine teleost in East Asia. We measured genome-scale gene expression of flounder haploid, gynogenetic diploid and normal diploid embryos using RNA-Seq, constructed a module-centric co-expression network based on weighted correlation network analysis(WGCNA) and analyzed the biological functions of correlated modules. Module gene content analysis revealed that the formation of gynogenetic haploids was closely related to the abnormality of plasma proteins, and the up-regulation of p53 signaling pathway might rescue gynogenetic embryos from haploid syndrome via regulating cell cycle arrest, apoptosis and DNA repair. Moreover, normal diploid has more robust nervous system. This work provides novel insights into molecular mechanisms in haploid syndrome and the rescue process by gynogenetic diploidization. 相似文献
15.
To solve the numerical divergence problem of the direct time domain Green function method for the motion simulation of floating bodies with large flare, a time domain hybrid Rankine-Green boundary element method is proposed. In this numerical method, the fluid domain is decomposed by an imaginary control surface, at which the continuous condition should be satisfied. Then the Rankine Green function is adopted in the inner domain. The transient free surface Green function is applied in the outer domain, which is used to find the relationship between the velocity potential and its normal derivative for the inner domain. Besides, the velocity potential at the mean free surface between body surface and control surface is directly solved by the integration scheme. The wave exciting force is computed through the convolution integration with wave elevation, by introducing the impulse response function. Additionally, the nonlinear Froude-Krylov force and hydrostatic force, which is computed under the instantaneous incident wave free surface, are taken into account by the direct pressure integration scheme. The corresponding numerical computer code is developed and first used to compute the hydrodynamic coefficients of the hemisphere, as well as the time history of a ship with large flare; good agreement is obtained with the analytical solutions as well as the available numerical results. Then the hydrodynamic properties of a FPSO are studied. The hydrodynamic coefficients agree well with the results computed by the frequency method; the influence of the time interval and the truncated time is investigated in detail. 相似文献
16.
赖泽兴 《广东海洋大学学报》1994,(1)
在常规催产剂中加入适量的Ａ型添加剂，可使鳜鱼的催产率提高１０％以上，受精率提高５％以上。使用１５×１０－６的福尔马林，可有效地杀灭寄生于鳃及体表的寄生虫和细菌，从而大大提高苗种成活率。 相似文献
17.
Sinonovacula constricta is one of the important economic aquaculture species in China. In this study, we constructed genetic linkage maps of S. constricta based on 300 microsatellite markers derived from RAD-seq using an F1 full-sib family. The female map contained 204 microsatellites assigned to 22 linkage groups, which covered 1529.5 cM with an average interval of 10.3 cM. The male consisted of 187 microsatellites in 19 linkage groups corresponding to the haploid chromosome number(n(28)19), which spanned 1429.3 cM with an average interval of 8.7 cM. The genome coverage was approximately 83.5% and 81.4%, respectively. An integrated map was constructed according to the common markers in parental linkage groups, which had a total length of 1683.8 cM with an average interval of 7.3 cM. The genome coverage of the integrated map was approximately 86.3%. The genetic linkage map would form the foundation for further studies on the quantitative trait loci(QTL), as well as accelerating the breeding process of this species. 相似文献
18.
采用室内水泥池及活饵培育配套技术进行尖吻鲈苗种阶段工厂化育苗。投放刚孵化出膜的仔鱼１６０万尾，经室内池８ｄ的培育，成活率为９０％；经１３ｄ的培育其规格超过１ｃｍ，成活率为５６．９％；经２４ｄ的培育平均规格达２ｃｍ左右，平均成活率为５０％，共育出８０万尾。苗种生长快，体质健壮，成活率高。关键技术是：基础设施配套；采用不同活饵，做到适口、及时转化、足量；定期过筛分级培育；水质控制良好；注意防病。２ｃｍ以上鱼种阶段采用多种方式培育，经１５～２０ｄ的饲养，规格达３～４ｃｍ，平均成活率为６５％～７５％。 相似文献
19.
Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents. 相似文献
20.
Toxic effects of zinc on four species of freshwater fish 总被引：1，自引：0，他引：1
The toxic effects of Zn2+ on Silver Carp (Hypophthalmichthyps molitrix C. et V.), Big Head Carp (Aristrichthys nobilis Richardson), Grass Carp (Ctenopharyngodon ilellus C. et V.), and Blunt Snout Bream (Megalobrama aimbly-cephala Yih) are studied. The test results are: (1) There are linear correlations between 24h LC50 and 48h LC50 of Zn2+ for Silver Carp fingerling and temperature. 24h LC50, 48h LC50 and 96h LC50 of Zn2+ for fry of the four species are also determined; (2) There are logarithmic correlations between the growth rates of the fry
and the concentrations of Zn2+ and between expansion of fish egg membranes after absorbing water and concentrations of Zn2+; (3) The tolerance of fry of the four species to Zn2+ is in the following order: Grass Carp>Silver Carp>Blunt Snout Bream>Big Head Carp; (4) The safe concentrations of Zn2+ are: Big Head Carp: 0.008 mg/L, Grass Carp: 0.046 mg/L, Blunt Snout Bream: 0.010 mg/L, Silver Carp: 0.012 mg/L, Silver Carp
fingerling: 0.09 mg/L. 相似文献