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1.
The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades, K. alvarezii, Kappaphycus sp., and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae. 相似文献
2.
ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｔｈｅｆｉｒｓｔｐａｐｅｒｏｆｔｈｉｓｓｅｒｉｅｓ (ＬｉａｎｄＬｉｕ ,2 0 0 2 ) ,ｗｅｒｅｐｏｒｔｅｄ 3ｇｅｎｅｒａｗｉｔｈ 7ｓｐｅｃｉｅｓｏｆＰｏｎｔｏｎｉｉｎａｅ,ｃｏｌｌｅｃｔｅｄｂｙｔｈｅＪｏｉｎｔＣｈｉｎｅｓｅ ＧｅｒｍａｎＭａｒｉｎｅＢｉｏｌｏｇｙＥｘｐｅｄｉｔｉｏｎｓｔｏＨａｉｎａｎＩｓｌａｎｄ(ｗｉｎｔｅｒｏｆ1 990ａｎｄｓｐｒｉｎｇｏｆ 1 992 ) .Ｔｈｅｐｒｅｓｅｎｔｐａｐｅｒｒｅｐｏｒｔｓａｎｏｔｈｅｒ 6ｇｅｎｅｒａ ,ｗｉｔｈ 9ｓｐｅｃｉｅｓ,ｆｒｏｍＨａ… 相似文献
3.
《中国海洋湖沼学报》2021,(2)
Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world.The morphological,physiological,and biochemical characte ristics are different amo ng geographic strains,which can not be distinguished with nuclear ribo somal DNA markers at present.Therefore,the genetic distance and phylogeographic relationships of nuclear 28 S rDNA D1-D2 and ITS regions,and three chloroplast intergenic spacers(petN-trnS1,trnM1-psbA,and rbcS-rpl27) were analyzed and compared among 13 strains of P.globosa isolated from the Pacific Ocean and Atlantic Ocean in this study.In addition,the nucleotide polymorphisms of 28 S rDNA D1-D2,ITS,and rbcS-rpl27 regions were evaluated in two P.globosa strains.The various levels of nucleotide polymorphism were in the nuclear 28 S rDNA D1-D2 region and ITS region,but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer.A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer,which had the strongest distinction to geographic strains compared to those of 28 S rDNA D1-D2 and ITS regions.In the phylogenetic tree of rbcS-rpl27 intergenic spacer,the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P.globosa,and then formed an independent clade,while the other Atlantic strains and all of Pacific strains j oined up to build the other clade.It was implied that at least two genetically distant populations of P.globosa existed in the Atlantic coastal regions.This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P.globosa,and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P.globosa. 相似文献
4.
1 INTRODUCTIONInthefirstandsecondpapersofthisseries (LietLiu ,2 0 0 2 ,2 0 0 3) ,wereported 9genera 1 6speciesofPontoniinae ,collectedbytheJointChinese GermanMa rineBiologyExpeditionstoHainanIsland (winterof1 990andspringof 1 992 ) .ThepresentpaperreportsonthespeciesofgenusPericlimenesfromHainanIslandcollectedbytheexpedition.ThematerialsstudiedherearealldepositedintheInstituteofOceanology ,ChineseAcademyofSciences,Qingdao,China (IOCAS) .Inthispaper,“Materialexamined”and“RN… 相似文献
5.
The presence of Biecheleria cincta (=Woloszynskia cincta) in the Chinese coasts is reported for the first time. In scanning electron microscope, three to five series of vesicles and an elongated apical vesicle (EAV) were visible in the epicone, and both the hypocone and the cingulum had three series of vesicles each. Thin sections revealed that B. cincta possesses stalked pyrenoids and an unusual eyespot consisting of a stack of cisternae with brick-like materials (type E), thus supporting its transfer from Woloszynskia to Biecheleria. Spiny cysts formed spontaneously in culture, with an encystment rate of around 20%. Both large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer region (ITS) sequences in 12 strains from the Chinese coasts were determined. Phylogenetic analyses based on LSU rDNA and ITS sequences using Bayesian inference and maximum likelihood revealed two distinct ribotypes (referred to as ribotype A and B) in B. cincta. ITS region pairwise distances within B. cincta ranged from 0.024 to 0.072, suggesting the existence of a complex of cryptic species. 相似文献
6.
ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｄｏ ＷｅｓｔＰａｃｉｆｉｃｗａｔｅｒｉｓｔｈｅｍａｉｎｄｉｓｔｒｉｂｕｔｉｏｎａｒｅａｏｆｓｈｒｉｍｐｓｏｆｔｈｅｓｕｂｆａｍｉｌｙＰｏｎｔｏｎｉｉｎａｅ ,ｗｈｅｒｅｍｏｒｅｔｈａｎ 60ｏｆｔｈｅ79ｃｕｒｒｅｎｔｌｙｒｅｃｏｇｎｉｚｅｄｐｏｎｔｏｎｉｉｎｅｇｅｎｅｒａｈａｖｅｂｅｅｎｆｏｕｎｄ .Ｔｈｅｒｉｃｈｐｏｎ ｔｏｎｉｉｎｅｆａｕｎａｏｆＨｏｎｇＫｏｎｇ ( 2 3ｓｐｅｃｉｅｓ,Ｂｒｕｃｅ ,1 982 ,1 990 ) ,ａｎｄｔｈｅｎｅｉｇｈｂｏｒｉｎｇｗａｔｅｒｓ,Ｐｈｉｌｉｐ ｐ… 相似文献
7.
琼东近海浮标污损生物研究 总被引：1，自引：0，他引：1
为了解南海北部近海石油开发区的污损生物状况,对海南岛东部近海海区的两个浮标及其锚锭系统上的污损生物进行了调查。结果表明,该海区污损生物的种类主要是藻类、水螅、有柄蔓足类和牡蛎;南海沿岸水域常见的无柄蔓足类仅出现在距海南岛相对较近的J2浮标站。并探讨了该海区的污损生物群落结构与邻近沿岸水域的差异。 相似文献
8.
Ulvacean green seaweeds are common worldwide; they formed massive green tides in the Yellow Sea in recent years, which caused
marine ecological problems as well as a social issue. We investigated two major genera of the Ulvaceae, Ulva and Enteromorpha, and collected the plastid rbcL and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed them. Phylogenetic trees
of rbcL data show the occurrence of five species of Enteromorpha (E. compressa, E. flexuosa, E. intestinalis, E. linza and E. prolifera) and three species of Ulva (U. pertusa, U. rigida and U. ohnoi). However, we found U. ohnoi, which is known as a subtropical to tropical species, at two sites on Jeju Island, Korea. Four ribotypes in partial sequences
of 5.8S rDNA and ITS2 from E. compressa were also found. Ribotype network analysis revealed that the common ribotype, occurring in China, Korea and Europe, is connected
with ribotypes from Europe and China/Japan. Although samples of the same species were collected from both sides of the Yellow
Sea, intraspecific genetic polymorphism of each species was low among samples collected worldwide. 相似文献
9.
The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island. 相似文献
10.
FAN Lijing) SUI Zhenghong) * MAO Yunxiang) and GUO Hao) ) Division of Life Science Technology Ocean University of China Qingdao P. R. China ) National Marine Environmental Monitoring Center State Oceanic Administration Dalian P. R. China 《中国海洋大学学报(英文版)》2007,6(1):33-39
1 Introduction Dinoflagellate Alexandrium has received intensive at-tention in recent years, as it is a valuable bioactive com-pound resource and related to harmful algal bloom (HAB)which has negative impact on marine ecology systemaquiculture, human health and tourism (Hallegraeff andBolch, 1992; Smayda, 1997; Vale and Antonia de MSampayo, 2001). However, not all of the species in genusAlexandrium produce toxin (Wendy et al., 2001), so rapididentification and clarification of the phyloge… 相似文献
11.
Chlorophyta species are common in the southern and northern coastal areas of China. In recent years, frequent green tide incidents in Chinese coastal waters have raised concerns and attracted the attention of scientists. In this paper, we sequenced the 18S rDNA genes, the internal transcribed spacer (ITS) regions and the rbcL genes in seven organisms and obtained 536-566 bp long ITS sequences, 1 377-1 407 bp long rbcL sequences and 1 718-1 761 bp long partial 18S rDNA sequences. The GC base pair content was highest in the ITS regions and lowest in the rbcL genes. The sequencing results showed that the three Ulva prolifera (or U. pertusa) gene sequences from Qingdao and Nan’ao Island were identical. The ITS, 18S rDNA and rbcL genes in U. prolifera and U. pertusa from different sea areas in China were unchanged by geographic distance. U. flexuosa had the least evolutionary distance from U. californica in both the ITS regions (0.009) and the 18S rDNA (0.002). These data verified that Ulva and Enteromorpha are not separate genera. 相似文献
12.
Wang Jianyan Cen Jingyi Li Si Lü Songhui Moestrup Øjvind Chan Kin-Ka Jiang Tao Lei Xiangdong 《中国海洋湖沼学报》2018,36(6):2202-2215
The dinoflagellate genus K arenia is known for recurrent harmful blooms worldwide. However, species diversity of the genus is generally overlooked owing to the difficulty of identifying small unarmored dinoflagellates. We have established four clonal cultures of K arenia longicanalis isolated from the type locality, Hong Kong harbor(strain HK01) and other three locations along the Chinese coasts(strains YB01, DT01, and NJ01). The morphology of the strain was studied by light and scanning electron microscopy(LM and SEM) and the pigment composition analyzed by high-performance liquid chromatography. We provide the first molecular data of K. longicanalis based on the large subunit(LSU) rRNA gene sequence and internal transcribed spacer(ITS). The four strains showed identical LSU rDNA sequences with a similarity of 99.4% to the holotype of K arenia umbella(strain KUTN05) from Australia. In the ITS phylogeny, the sequence of K. umbella branched between the Chinese strains of K. longicanalis. A careful comparison of the morphology of K. longicanalis and K. umbella reveals the similarity in the diagnostic characters. Diff erences may appear due to the sample treatment for SEM. We conclude that K. umbella is a junior synonym of K. longicanalis. 相似文献
13.
MODUcTIONThislistof4pisionidspedesisoneofthecontributi0nstothestudyoflittofalmeiofaunaIp0lychaetesfromtheChinesecoast.ThernateriaIswerecoforduringashortexpeditiontoHainanIslandbyProfB.L.Wu,Mr.F.P.HuangandMr.Z.H.ixngoftheFirstInstituteofdrinography,StatedrinicAdndnisttationinQingdaoandProfDr.W.WeStheideandDr.G.PurschkeoftheUniversityofDenabruckinOct0berl99l,additionalmateriaIsweresamPledbyMr.Z.H.Dinginash0rtexpeditiontoHongkonginJanuary1995.Hithert0,onlyPtrionelevisetoSaZhao… 相似文献
14.
Miamiensis avidus Thompson Moewus,1964,is a cosmopolitan and well-known marine pathogenic ciliated protist.However,the taxonomy of this species up to now has remained controversial,especially with respect to the validity of the morphologically similar species,Philasterides dicentrarchi,which was considered as a junior synonym of M.avidus.In this study,a population of M.avidus was collected from the skin of pharaoh cuttlefish(Sepia pharaonis) cultured near the East China Sea,Ningbo,China and its morphology and phylogeny were investigated in detail based on living characters,infraciliature,small subunit(SSU) r DNA and ITS1-5.8 S-ITS2 region sequences.In addition,the morphometrics of a previously reported free-living population,collected from the Bohai Sea,were rechecked and analyzed.We compared the present two isolates with all historic populations of M.avidus and P.dicentrarchi,and found that their morphological characters were either highly similar or exactly identical,indicating that they are the same morphospecies.However,the phylogenetic analyses based on SSU r DNA or ITS1-5.8 S-ITS2 region sequences revealed that most M.avidus and P.dicentrarchi populations formed one clade,and the two isolates of M.avidus from Weifang and American Type Culture Collection clustered in another clade,which indicated that there might be cryptic species in Miamiensis avidus. 相似文献
15.
LIU Hongquan ) ) YU Wengong ) * DAI Jixun ) GONG Qianhong ) SHI Xiaochong ) YANG Kunfeng ) ) Institute of Marine Drugs Foods Ocean University of China Qingdao P..R.China ) College of Marine Life Sciences Ocean University of China Qingdao P..R.China 《中国海洋大学学报(英文版)》2004,3(1)
1 Introduction PorphyraisthemainobjectforalgafarmingandplaysaveryimportantroleinChinesemarineindus tries.Recently ,therehasbeenagreatlossinPor phyracultivationduetothedegenerationoftheculti var,sothereisanincreasingdemandforgoodPor phyracultivars .Theprerequisiteofthetraditionalbreed ingandbioengineeringresearchofPorphyraistheconstructionofpurelines .Traditionally ,theclassifi cationofPorphyrawasaccordingtotheirmorphologi calcharacteristics .However ,mostmorphologicalfea turesofPorphyraar… 相似文献
16.
Numerical simulation of the structure and variation of upwelling off the east coast of Hainan Island using QuikSCAT winds 总被引：3，自引：0，他引：3
The spatial structure and variation of the upwelling in the waters east and northeast of Hainan Island, China during 2000-2007 were investigated using a nested high-resolution Princeton Ocean Model (POM) forced by QuikSCAT winds. The model produced good simulations of the summer upwelling and the seasonal and annual variability. Strong upwelling occurs from mid-July to mid-August with a peak east of Hainan Island associated with the southwesterly monsoon in the South China Sea. Sensitivity experiments indicated that when the local wind stress controls the variability of the upwelling, the large-scale circulation significantly enhances the upwelling northeast of Hainan Island by inducing a local upwelling and transporting cold water northeast-ward along the island’s east coast. The joint effects of the local wind stress and large-scale circulation result in stronger upwelling northeast of Hainan Island. This implies that the annual variation of the upwelling northeast of Hainan Island is controlled not only by the local alongshore wind stress but also by the large-scale circulation. This result will help us investigate the decadal variation of the upwelling in this region in the future. 相似文献
17.
The present paper reports 12 species of genus Periclimenes, subfamily Pontoniinae, collected from Hainan Island, South China Sea, by the Joint Chinese-German Marine Biology Expeditions, in which, Periclimenes consobrinus and P. tenuipes are new records from Chinese waters, and Periclimenes amymone, P. holthuisi, P. soror, and P. toloensis are recorded for the first time from Hainan Island. 相似文献
18.
We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS （internal transcribed spacer） and 5S rDNA NTS （non-transcribed spacer） sequences. The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera. In contrast, multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa. In general, there were more variable sites in the NTS region than in the ITS region in the three species. The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high. However, a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution. 相似文献
19.
The present paper reports 7 species of pontoniine shrimps collected from Hainan Island,South China Sea,by the Joint Chinese-German Marine Biology Expeditions,in which,Conchodytes nipponensis(De Haan,1844)and Coralliocaris brevirostris Borradaile,1898 are new records from Chinese waters. 相似文献
20.
The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated.The results are as follows:the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains.The sequences of ITS1 were 331 bp to 334 bp,while those of the 5.8... 相似文献