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Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007 总被引：3，自引：1，他引：2
Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. 相似文献
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Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular
phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not
the biogeographic origin of the free-floating Enteromorpha in that season.
Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology
Project of Qingdao City (06-2-2-12-JCH) 相似文献
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Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium. 相似文献
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Ulvacean green seaweeds are common worldwide; they formed massive green tides in the Yellow Sea in recent years, which caused
marine ecological problems as well as a social issue. We investigated two major genera of the Ulvaceae, Ulva and Enteromorpha, and collected the plastid rbcL and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed them. Phylogenetic trees
of rbcL data show the occurrence of five species of Enteromorpha (E. compressa, E. flexuosa, E. intestinalis, E. linza and E. prolifera) and three species of Ulva (U. pertusa, U. rigida and U. ohnoi). However, we found U. ohnoi, which is known as a subtropical to tropical species, at two sites on Jeju Island, Korea. Four ribotypes in partial sequences
of 5.8S rDNA and ITS2 from E. compressa were also found. Ribotype network analysis revealed that the common ribotype, occurring in China, Korea and Europe, is connected
with ribotypes from Europe and China/Japan. Although samples of the same species were collected from both sides of the Yellow
Sea, intraspecific genetic polymorphism of each species was low among samples collected worldwide. 相似文献
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藻类是光合自养的水生孢子植物,为了适应水下弱光的特殊生境,藻类捕光天线历经亿万年的进化,形成了特殊的结构与功能。从发现藻类捕光天线的存在到至今的70多年间,其结构解析技术的发展共经历了4个阶段:首先是利用生化及普通光谱技术研究结构组成(1950?1980年);其次是利用X-ray晶体学技术研究局部精细结构(1980年至今);再次是利用电镜技术研究完整的粗略结构(1980?2010年);最后是近10年来利用冷冻电镜技术研究完整的精细结构(2010年至今)。目前以蓝藻、红藻、绿藻和硅藻为主的藻类捕光天线复合体完整的精细结构均已被解析,仅2019年就有10余种精细结构被发现。藻类捕光天线系统结构生物学的研究,不仅搭建了对结构与功能统一认识的桥梁,而且为深入揭示藻类光合作用高效能量传递机制奠定了坚实的结构基础。将藻类捕光天线系统结构和功能统一起来,进一步研究对光环境的适应性成为未来的重点,并将为藻类捕光天线蛋白在光电器件领域的应用提供充分的科学依据。 相似文献
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