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1.
The haematological, biochemical, and enzymological alterations produced on exposure of Channa punctatus to LC50 (11.2 mg/L) for 96 h and to a sublethal concentration (1.12 mg/L) of cadmium (Cd2+) for 15, 30, 60, and 120 days have been studied. The fish were hypoglycemic and hypolactemic. The pyruvate content of blood and liver decreased in acute and all stages of chronic exposure except for 30 days where significant increase was recorded. Depletion was noted in the total protein and glycogen content of liver and muscle, and the level of lactate in liver in the two types of exposure. The levels of muscle lactate and pyruvate increased in both exposures. The activities of hexokinase, glucose-6-phosphatase, and lactate dehydrogenase decreased in liver after 96 h and all stages of chronic exposure except 30 days. The activities of hexokinase and lactate dehydrogenase in muscle and glutamate-oxalacetate transaminase and glutamate-pyruvate transaminase in serum, liver, and muscles increased in all exposures. The activities of succinate and malate dehydrogenases in liver and muscles decreased after acute and all stages of chronic exposure. The above results suggest that glycolysis and gluconeogenesis were impaired in liver but glycolysis prevailed in muscle. Aerobic oxidation is adversely affected in metal exposed fish, while proteolysis was increased. 相似文献
2.
The lethal and sublethal effects of the xenoestrogen 4-nonylphenol (NP) were evaluated in the cockle Cerastoderma glaucum. In a 96-h lethality test, bivalves were exposed to 0, 0+ acetone, 0.19, 0.38, 0.75, 1.5 and 3.0 mg NP/l. The 96-h LC(50) value was 0.3mg NP/l. No mortality was observed at 0.1 mg NP/l. The potential estrogenicity of NP was studied in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) cockles, exposed for 7 and 14 days to 0, 0+ acetone, 0.0125, 0.025, 0.05, and 0.1 mg NP/l. Vitellogenin (Vg)-like protein levels were determined in both haemolymph and digestive gland by the alkali-labile phosphate (ALP) assay. In the resting phase, exposure for 7 days to 0.1 mg NP/l resulted in significant increases in ALP in both haemolymph and digestive gland, compared with controls. A significant increase was also observed in digestive gland of animals exposed to 0.0125 mg NP/l-exposed animals. After 14 days of exposure, haemolymph ALP levels were significantly increased in exposed animals at all NP concentrations tested, whereas no difference was recorded in digestive gland. In the pre-spawning phase, exposure for 7 days to NP significantly increased ALP levels in haemolymph from males exposed at all NP concentrations tested, whereas no significant variations were found in haemolymph from females. NP (0.05 and 0.1 mg/l) was also shown to increase ALP concentrations significantly in digestive gland of males, but not in those of females. Likewise, after 14 days' exposure, ALP levels significantly increased in haemolymph from males only at 0.1 mg NP/l. Conversely, NP caused significant increases in ALP levels in digestive gland from both males (at all NP concentrations tested) and females (at 0.025 and 0.1 mg NP/l). These results demonstrate that NP induces Vg synthesis in C. glaucum. Interestingly, males were more responsive to NP than females. 相似文献
3.
Maria Gabriella Marin Stefano Rigato Francesco Ricciardi Valerio Matozzo 《Marine pollution bulletin》2008,57(6-12):552
The lethal and sublethal effects of the xenoestrogen 4-nonylphenol (NP) were evaluated in the cockle Cerastoderma glaucum. In a 96-h lethality test, bivalves were exposed to 0, 0+ acetone, 0.19, 0.38, 0.75, 1.5 and 3.0 mg NP/l. The 96-h LC50 value was 0.3 mg NP/l. No mortality was observed at 0.1 mg NP/l. The potential estrogenicity of NP was studied in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) cockles, exposed for 7 and 14 days to 0, 0+ acetone, 0.0125, 0.025, 0.05, and 0.1 mg NP/l. Vitellogenin (Vg)-like protein levels were determined in both haemolymph and digestive gland by the alkali-labile phosphate (ALP) assay. In the resting phase, exposure for 7 days to 0.1 mg NP/l resulted in significant increases in ALP in both haemolymph and digestive gland, compared with controls. A significant increase was also observed in digestive gland of animals exposed to 0.0125 mg NP/l-exposed animals. After 14 days of exposure, haemolymph ALP levels were significantly increased in exposed animals at all NP concentrations tested, whereas no difference was recorded in digestive gland. In the pre-spawning phase, exposure for 7 days to NP significantly increased ALP levels in haemolymph from males exposed at all NP concentrations tested, whereas no significant variations were found in haemolymph from females. NP (0.05 and 0.1 mg/l) was also shown to increase ALP concentrations significantly in digestive gland of males, but not in those of females. Likewise, after 14 days’ exposure, ALP levels significantly increased in haemolymph from males only at 0.1 mg NP/l. Conversely, NP caused significant increases in ALP levels in digestive gland from both males (at all NP concentrations tested) and females (at 0.025 and 0.1 mg NP/l). These results demonstrate that NP induces Vg synthesis in C. glaucum. Interestingly, males were more responsive to NP than females. 相似文献
4.
The present paper deals with the toxic effects if any of the two concentrations of Arsenic(III)-oxide on the nucleic acids and protein metabolism in the liver of H. fossilis after exposure for 20 and 30 days. Causes for significant alterations have been discussed. 相似文献
5.
《Marine pollution bulletin》2009,58(6-12):552-558
The lethal and sublethal effects of the xenoestrogen 4-nonylphenol (NP) were evaluated in the cockle Cerastoderma glaucum. In a 96-h lethality test, bivalves were exposed to 0, 0+ acetone, 0.19, 0.38, 0.75, 1.5 and 3.0 mg NP/l. The 96-h LC50 value was 0.3 mg NP/l. No mortality was observed at 0.1 mg NP/l. The potential estrogenicity of NP was studied in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) cockles, exposed for 7 and 14 days to 0, 0+ acetone, 0.0125, 0.025, 0.05, and 0.1 mg NP/l. Vitellogenin (Vg)-like protein levels were determined in both haemolymph and digestive gland by the alkali-labile phosphate (ALP) assay. In the resting phase, exposure for 7 days to 0.1 mg NP/l resulted in significant increases in ALP in both haemolymph and digestive gland, compared with controls. A significant increase was also observed in digestive gland of animals exposed to 0.0125 mg NP/l-exposed animals. After 14 days of exposure, haemolymph ALP levels were significantly increased in exposed animals at all NP concentrations tested, whereas no difference was recorded in digestive gland. In the pre-spawning phase, exposure for 7 days to NP significantly increased ALP levels in haemolymph from males exposed at all NP concentrations tested, whereas no significant variations were found in haemolymph from females. NP (0.05 and 0.1 mg/l) was also shown to increase ALP concentrations significantly in digestive gland of males, but not in those of females. Likewise, after 14 days’ exposure, ALP levels significantly increased in haemolymph from males only at 0.1 mg NP/l. Conversely, NP caused significant increases in ALP levels in digestive gland from both males (at all NP concentrations tested) and females (at 0.025 and 0.1 mg NP/l). These results demonstrate that NP induces Vg synthesis in C. glaucum. Interestingly, males were more responsive to NP than females. 相似文献
6.
The influence of Pacific salmon decay products on near‐field streambed sediment and organic matter dynamics: a flume simulation 
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下载免费PDF全文 Pacific salmon are biogeomorphic agents shown to induce positive feedbacks on their natal watersheds. However, the literature documenting their ecological effects on in‐stream natal environments is more divisive. The disturbance salmon create during redd construction has the potential to reduce stream productivity. The pulse of salmon organic matter (SOM) and marine derived nutrients (MDNs) released during carcass decay has been reported as either stimulating in‐stream productivity or having no local effect. To evaluate the ecological costs and benefits of salmon spawning events, MDN delivery and storage processes need to be identified and quantified. A simulation was conducted in three flow‐through flumes (2 m × 2 m × 30 m) over a 33‐day period (consisting of 15 baseline, four MDN exposure, and 14 post‐exposure days) to assess near‐field sediment and organic matter dynamics during active and post‐spawn simulations. The objective of the study was to measure changes in the amounts and particle sizes of suspended and gravel‐stored fine sediment, in order to elucidate the process and significance of SOM recruitment to the gravel bed via sedimentation. Gravel beds in all flumes were enriched with SOM following treatments but the response was highest in the active spawn simulation. The more effective delivery in the active spawn simulation was attributed to its higher inorganic sediment concentration, which is known to enhance floc formation. Although the active spawn simulation delivered more SOM to the gravel bed, the post‐spawn phase may be equally important to natural streams because its decay phase is longer than the active spawn and consequently can provide SOM to the streambed as long as carcasses remain in‐stream. The delivery, and potential retention, of SOM to spawning streambeds and the intergravel environment may be particularly important for interior streams, which experience low flow conditions during the spawning phase and accordingly have the potential for hyporheic nutrient recruitment and storage. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
7.
Ching EW Siu WH Lam PK Xu L Zhang Y Richardson BJ Wu RS 《Marine pollution bulletin》2001,42(7):603-610
Green-lipped mussels, Perna viridis, were exposed to 0, 0.3, 3 and 30 micrograms l-1 (nominal concentrations) B[a]P under laboratory conditions over a period of 24 days. Mussels were collected on day 0, 1, 3, 6, 12, 18 and 24, and the levels of DNA adducts and DNA strand breaks in their hepatopancreas tissues monitored. Mussels exposed to 0.3 and 3 micrograms l-1 B[a]P showed marked increases in strand breaks after 1 day of exposure. DNA strand break levels in these mussels remained high and significantly different from the control values until day 3 for the 0.3 microgram l-1 treatment group, and day 6 for the 3 micrograms l-1 treatment group. This was followed by a gradual reduction in strand breaks. After 12 days, the levels of both groups had returned to the same level as that of the control. No increase in DNA strand breaks was observable in mussels exposed to 30 micrograms l-1 B[a]P in the first 12 days of exposure, but a significant increase was observed from day 12 to day 24. Increasing B[a]P concentrations resulted in elevated DNA adduct levels after 3-6 days of exposure, but this pattern of dose-related increase disappeared after 12 days. These results indicate that a better understanding of the complex interactions between exposure levels and durations is crucially important before DNA adduct levels and DNA strand breaks in P. viridis can be used as effective biomarkers for monitoring genotoxicants in marine waters. 相似文献
8.
依托中蒙国际合作项目"远东地区地磁场、重力场及深部构造观测与模型研究",我们首次获取了蒙古中南部地区密集的地震台阵观测资料.本研究收集了69套宽频带台站2011年8月至2013年7月间的垂直向连续记录,利用噪声互相关方法计算了台站间的经验格林函数,并采用基于连续小波变换的频时分析方法,提取了1478条周期6~30s的瑞雷波的相速度频散曲线.利用DitmarYanovskaya方法,我们构建了研究区6~30s瑞雷波的高分辨率(0.5°×0.5°)相速度分布图.结果表明,蒙古中南部地区的地壳上地幔速度结构存在横向非均匀性,但并不显著(相对变化~±2%).短周期(如6s)的相速度分布与地表地质构造具有明显的相关性,具体来说,北部山盆显示为高速,南部盆地、戈壁滩均显示为低速;随着周期的增大(如15s,20s),地形的控制作用相对减弱.较长周期(30s)的相速度分布图上,南部的高速区进一步扩大,与南部地壳厚度较薄有关.蒙古主构造线南北两侧相速度分布有明显差异,暗示它不仅是地表地形和构造的分界线,而且还是地壳结构的分界线.从6~30s中戈壁一直显示为低速,可能与该区新生代火山活动有关;研究区北部杭爱山—肯特盆地一直显示为相对的高速,与该区具有较老、稳定的地层有关. 相似文献
9.
王海 《CT理论与应用研究》2001,10(2):30-32
目的：探讨小肝细胞癌和肝脏小血管瘤螺旋CT增强表现及其随时间延迟所致的CT征象的变化过程，材料与方法：50例患者（15例SHCC，35例SHHE），其中19例经手术病理标本证实，其余患者由临床表现和实验室检查诊断，采用Siemens Plus4C螺旋CT机，先作平扫，然后在肿瘤靶平面平衡期扫描。着重观察，分析不同CT扫描时相小肝细胞癌和肝脏小血管瘤CT表现特征的动态变化过程，结果，SHHE组发现病灶44个，30个病灶在动脉期动缘明显强化逐渐向中心填充，22个病灶在动脉期和门静脉期均为高密度，2个病灶在动脉期和门静脉期均为底密度，在平衡期42个病灶SHHE为高密度和等密度SHCC组发现二18个，14个病灶SHCC显示动脉期呈高密度、门静脉期为低密度，3个病灶动脉期为高密度、门静脉期为等密度、1个病灶动脉期、门静脉期均为低密度，平衡期16个病灶的SHCC为低密度，结论：根据SHHE和SHCC在螺旋CT多期扫描的强化表现，大多数病例可明显两者诊断。 相似文献
10.
J. P. Shukla 《洁净——土壤、空气、水》1988,16(6):621-624
The effect of a sublethal concentration of malathion (95% E. C.) on the nucleic acids (DNA, RNA) and protein content in the liver of fingerlings of Channa punctatus, a freshwater murrel was investigated. 10 days of exposure brought non-significant alteration (P < 0.05) neither in the nucleic acids nor in protein content. However, significant quantitative decline in the liver's RNA (P < 0.01) and protein (P < 0.001) but not in the DNA content was observed after 20 days of exposure. Possible causes for such decline have been discussed. 相似文献
11.
Green-lipped mussels (Perna viridis) were exposed to Aroclor 1254 (0.5, 5 and 50 microgl(-1)) and a mixture of benzo[a]pyrene (B[a]P) and Aroclor 1254 (0.3+0.5 and 3+5 microgl(-1)) for 12 days. On day 0, 1, 3, 6 and 12, the levels of DNA strand breaks in the mussel hepatopancreas were monitored using an alkaline unwinding assay. The results were compared to the findings of a previous study in which the levels of DNA strand breakage in the same species were measured following exposure to various concentrations of B[a]P (0.3, 3 and 30 microgl(-1)). The results indicated that Aroclor 1254 at a concentration 相似文献
12.
基于2021年5月21日漾濞M_S6.4地震震中附近50 km范围内15个地震观测台站记录的2020年11月1日—2021年5月30日的气枪震源信号,采用互相关时延检测技术提取这15个台站各自稳定震相(Sg震相)的走时变化时间序列,并对漾濞M_S6.4地震前后各台站的走时变化特征进行分析。结果表明:(1)漾濞M_S6.4地震前10天左右,有4个台站走时开始出现异常,其中,Ey211和Ey213台走时呈"V"型变化结构,Ey210和CHT台走时呈上升趋势。(2)漾濞M_S6.4地震后短期内,大部分台站走时出现了明显的趋势性变化,以维西—乔后—巍山断裂为界,断裂带两侧台站走时变化趋势存在明显差异,断裂以东的EYA、HDQ、YSW09台走时呈上升趋势,变化最为显著的为HDQ台,走时差变化量为0.067 s;而断裂带以西的所有台站在漾濞M_S6.4地震当天(或震后1～2天)开始出现明显趋势性下降,走时下降持续时间在4～9天间,走时差变化量在-0.053～-0.201 s间,其中走时差下降量最大为53 285台,最小为CHT台。 相似文献
13.
Fingerlings of Channa punctatus, a freshwater murrel, when subjected to a sublethal concentration of 12 mg l?1 zinc for 20 days elicited a significantly decreased (P<0.01) RNA content and hypoproteinemia in their liver. However, an exposure for 10 days under the same condition did not produce a significant alternation (P>0.05) neither in the nucleic acids (RNA – DNA) nor in the protein content. Results are discussed in relation to the pollutional stress of this heavy metal. 相似文献
14.
Mimura H Sato R Furuyama Y Taniike A Yagi M Yoshida K Kitamura A 《Marine pollution bulletin》2008,57(6-12):877-882
The isolate, Pesudoalteromonas sp. TBT1, could grow to overcome the toxicity of tributyltin chloride (TBTCl) up to 30 microM in the absence of Cl(-) in the medium until the cells reached an exponential phase of growth. The viability, however, was reduced after the cells reached a stationary phase. The degradation products, such as dibutyltin (DBT) and monobutyltin (MBT), were not detected in the growth medium, indicating that the isolate has no ability to degrade TBT into less toxic DBT and MBT. Up to about 10(7.5) TBT molecules were adsorbed by a single cell. The observation of morphological changes with an electron microscope showed that the cell surface became wrinkled after exposure to the lethal concentration of 10 mM TBTCl. These results indicate that the resistance of the isolate toward the toxicity of TBTCl is not related to the unique cell surface, which seems to play an important role in preventing the diffusion of TBTCl into the cytoplasm. 相似文献
15.
This study assessed the sensitivity of the amphipod Chaetocorophium cf. lucasi, an inhabitant of New Zealand estuarine muddy sediments, and of the bivalve Macomona liliana, commonly found in New Zealand sandflats, to sediment particle-size distribution, total organic carbon, total organic nitrogen and water content. Growth and survival were evaluated after different test exposure times using laboratory-prepared natural sediment mixtures of sand and mud. The results suggest that amphipod survival was affected by the type of sand more than by the concentration of sand in the sediment samples. In samples with high survival rates, amphipod growth was impaired in sediments with higher sand proportions after a 10-day exposure, but after longer periods (28 or 30 days) there were no significant differences among treatments which had ≤76% sand. Bivalve survival and growth were not significantly affected by different natural sediment characteristics. Test duration for chronic toxicity tests with the bivalve M. liliana and the amphipod C. cf. lucasi should be at least 28 days for the detection of significant growth relative to the initial size. 相似文献
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Although lignin is known to be not readily biodegradable the concentration of dissolved lignin decreased during aerobic biological treatment of paper mill wastewater performed in sequencing batch reactors (SBR). Systematic lab scale batch tests were conducted to clarify whether the observed removal of lignin was the result of biodegradation or adsorption onto the activated sludge. For the batch tests, sludge samples were taken from sequencing batch reactors operated at solid retention times (SRT) of 10, 15, 20, 30, and 40 days, respectively. The amount of lignin present in the bulk liquid and in the sludge samples was quantified by an analytical procedure comprising pyrolysis, gas chromatography and mass spectrometry (py‐GC/MS analysis). It was found that lignin adsorbs onto the activated sludge by up to 30%[TH]w/w. This demonstrates the sludge excellent adsorption properties. The ultimate removal of lignin is achieved by sludge wasting. The highest overall removal rate was found when sludge was used from the SBR run at SRT of 20 days. 相似文献
19.
Lamellidens marginalis (Molluska Bivalvia Eulamellibranchiata) is an important member of the freshwater ecosystem of India, which is sustained by filtering phytoplankton, bacteria and other particulate organic matter from the available water. Hemocytes, the circulating blood cells of bivalves, function as immunological effector cells under exposure to toxins and/or parasites. The hemocytes of Lamellidens marginalis have been identified morphologically and enumerated as being qualified for use as a biomarker to analyze the threat of freshwater contamination by sublethal concentrations of sodium arsenite, a natural pollutant of freshwater ecosystems. Diverse subpopulations of hemocytes were identified as blast‐like cells, granulocytes, agranulocytes, hyalinocytes and asterocytes. Supression in the total count of hemocytes and blast‐like cells was recorded under the exposure of all the concentrations of sodium arsenite tested. Sodium arsenite elevated the relative density of granulocytes, hyalinocytes, and asterocytes. Partial restoration of the total count of the hemocytes was recorded after the post‐treated animals were maintained in arsenic‐free water for periods of 15 and 30 days. The data predict a shift in the immunological parameters of this bivalve in arsenic contaminated environments. The present study is aimed at quantifying the arsenic induced stress in Lamellidens marginalis and establishing the hemocyte density as a biomarker of aquatic pollution in selected geographical regions of India. 相似文献
20.
Aspects of the usefulness of five marine pollution biomarkers, with emphasis on MN and lipid content
By using cellular biomarkers, such as the formation of the micronucleus, the morphometric alterations of lipids and the morphometric changes in the lysosomal system, we investigated effects of experimental exposure to phenanthrene, Cu, Cd, and Hg for 15 days in mussels. Concerning micronuclei, the evaluation of the total nuclear abnormalities, instead of the micronucleus only, as a biomarker of marine pollution, indicated more statistically significant differences between the control and the pollutant treated groups. Contrary to the existing knowledge showing that there is an increase in lipid content after pollutant exposure, our results showed that there was a decrease in the amount of lipid, as well as an increase in the number of neutral lipids. Furthermore, although prior studies found that fewer lysosomes formed after pollutant exposure, this was not confirmed by our work, as pollutant treated animals exhibited a decrease in the volume and an increase in the numerical density of lysosomes, compared to control groups. 相似文献