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用解剖镜镜检的方法鉴定和分析了2001年2、5、8月,2002年5、8、11月以及2003年2月在胶州湾7个航次中浅海Ⅲ型网垂直拖网样品中的砂壳纤毛虫。结果表明,各个航次优势种(丰度)为:2001年2月,Tintinnopsis brasiliensis1500-23900个/m3;2001年5月,Codonellopsis mobilis80600-624000个/m3,Leprotintinnus neriticus920-13842个/m3,Fav-ella campanula184-2728个/m3,T.kofoidi0-368个/m3;2001年8月,Stenosemella sp.0-982个/m3,T.radix98-19114个/m3,L.neriticus0-5932个/m3,E.panamensis0-1318个/m3,T.gracilis0-317个/m3,L.nordqvisti O-158个/m3;2002年5月,C.mobilis22400-1567500个/m3,L.neriticus900-96300个/m3,C.parvicollis0-75600个/m3;2002年8月,E.panamensis0-2604个/m3,C.mobilis0-992个/m3,T.gracilis0-256个/m3,L.nordqvisti0-102个/m3;2002年11月,C.ostenfeldi0-13003个/m3,L.neriticus0-713个/m3,T.schotti0-316个/m3;2003年1月,砂壳纤毛虫丰度低干50个/m3。下列5种是新记录种:C.ostenfeldi,T.schotti,T.kofoidi,L.neriticus,Stenosemella sp.。 相似文献
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CC趋化因子受体3（CCR3）是CC型趋化因子的受体,属于G蛋白耦联受体（GPCRs）家族成员,与过敏等多种炎性疾病密切相关。为探讨香鱼CCR3（PaCCR3）在响应鳗弧菌感染时的表达变化,从转录组测序数据中获得了PaCCR3基因全长cDNA序列。序列分析表明,PaCCR3具有7次跨膜结构,与胡瓜鱼CCR3氨基酸序列同源性最高（84.6%）。系统进化树分析表明,哺乳类、两栖类和鱼类CCR3单独成簇,鱼类CCR3形成一个大簇;PaCCR3与胡瓜鱼CCR3进化相关性最高。实时荧光定量PCR结果显示,PaCCR3基因mRNA在单核/巨噬细胞（MO/MФ）中表达量最高;鳗弧菌感染后肝、脾和头肾中PaCCR3基因mRNA表达量显著上调;且香鱼MO/MФ经鳗弧菌体外刺激后,PaCCR3基因mRNA的表达量显著上调。Western blot分析表明经鳗弧菌体外刺激后,香鱼MO/MФ中PaCCR3蛋白的表达量也显著增加。综上,PaCCR3基因mRNA及其编码蛋白响应于病原体感染而被诱导表达。研究结果首次揭示了香鱼CCR3基因mRNA及蛋白的表达响应于鳗弧菌侵染,它可能通过介导MO/MФ的功能活性参与免疫应答,可为深入探究鱼类CCR3的功能及作用机制提供新的切入点。 相似文献
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通过克隆大黄鱼(Larimichthys crocea)的EBI3(Epstein-Barr virus-induced gene 3)基因并对其进行了序列分析.大黄鱼EBI3(Lyc EBI3)基因开放阅读框长747bp,编码248个氨基酸,存在1个信号肽序列和2个FN3结构域(37~130,145~230).多序列比对发现Lyc EBI3分子与其他已知EBI3氨基酸水平一致性为27.68%~57.53%.Real-time PCR结果表明,Lyc EBI3基因在脾脏和血液中表达量最高,且溶藻弧菌(Vibrio alginolyticus)刺激后,大黄鱼头肾和脾脏中Lyc EBI3基因的转录水平会显著升高,说明Lyc EBI3基因可能参与了抑制由细菌引起的炎症反应. 相似文献
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离子色谱-电感耦合等离子体质谱联用测定海水中的IO3^-和I^- 总被引：1，自引：0，他引：1
研究了离子色谱-电感耦合等离子体质谱（IC-ICPMS）联用技术直接进样测定海水中IO3^-和I^-的方法．采用IonPacAG23离子色谱保护柱分离IO3^-和I^-，以浓度4．0mmol／dm3的KOH为流动相，流量为1．0cm3／min，每个样品的分析时间为2．5min．采用2．5×10^-3cm^3的进样量，IO3^-和I^-的检出限分别达到0．6和0．4nmol／dm2，可满足海水中碘形态的定量分析．该方法的IO3^-和I^-浓度范围在2．0nmol／dm3～2．0μmol／dm3． 相似文献
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3-(3′-羟基)丁基苯酞是丁苯酞在体内的代谢产物,具有多种药理作用,可以作为一种新的活性成分应用于药物开发中。本文以甘氨酸、甲硫氨酸和亮氨酸为起始原料,经过Boc保护,再与3-(3′-羟基)丁基苯酞进行缩合,脱保护3步反应合成了3-(3′-羟基)丁基苯酞的氨基酸酯。以期提高水溶性和改善药代动力学性质,获得3-(3′-羟基)丁基苯酞的前药。 相似文献
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本文对TOA萃取剂萃取钍适宜的操作条件及萃取溶剂进行了试验,提出了TOA/苯萃取234Th的最佳条件: 在 1cm3含 234Th的 6mol/dm3 HNO3 溶液中,用体积比为 0. 5的TOA/苯溶液 2cm3 萃取,然后用 2cm3 1. 0mol/dm3 H2SO4反萃取,最高萃取率达 94%.对TOA/苯、TBP/煤油和TTA/苯这 3种萃取体系的萃取效率及其影响因素、萃取机理、成本等做了比较,认为 3种体系各有利弊,但均可有效地应用于相关的海洋学研究中. 相似文献
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ZHANG Naixing SONG Jinming CAO Conghu REN Rongzhu WU Fengcong ZHANG Shaoping SUN Xu 《海洋学报(英文版)》2012,31(1):73-82
The influence of macronitrogen (NO - 3 and NH + 4 ) addition with Ulva pertusa on dissolved inorganic carbon system in seawater was studied. The results indicate that p(CO 2 ) and HCO 3 concentration decrease significantly, while pH and CO 2- 3 concentration increase significantly. When the concentration of NO 3 was less than 71 μmol/dm 3 or NH + 4 was less than 49.7 μmol/dm 3 , dissolved inorganic carbon (DIC) absorption rates by Ulva pertusa generally increased with the increasing of nitrogen concentration. The DIC decreased 151 μmol/dm 3 with the addition of 71 μmol/dm 3 NO 3 and decreased 232 μmol/dm 3 with the addition of 49.7 μmol/dm 3 NH + 4 after the experiment compared with DIC measured without nitrogen addition. A significant negative-correlation was found between c(DIC) and growth rate (μ) of Ulva pertusa (r = -0.91, P <0.000 1, n=11). NH + 4 had more influence on the species of inorganic carbon system than NO 3 . 相似文献
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南黄海和中国东海中挥发性卤代烃的分布与海气通量 总被引：1，自引：0，他引：1
Distributions and sea-to-air fluxes of five kinds of volatile halocarbons(VHCs) were studied in the southern Yellow Sea(SYS) and the East China Sea(ECS) in November 2007. The results showed that the concentrations of 1,1,1-trichloroethane(C2H3Cl3), 1,1-dichloroethene(C2H2Cl2), 1,1,2-trichloroethene(C2HCl3), trichloromethane(CHCl3) and tetrachloromethane(CCl4) in the surface water were 0.31–4.81, 2.75–21.3, 1.21–17.1, 5.02–233 and 0.045–4.47 pmol/L, respectively, with the average values of 1.89, 12.20, 6.93, 60.90 and 0.33 pmol/L. On the whole, the horizontal distributions of C2H3Cl3, C2H2Cl2 and CCl4 were affected mainly by anthropogenic activities, while C2HCl3 and CHCl3 were influenced by biological factors as well as anthropogenic activities. In the study area, the concentrations of VHCs(except C2HCl3) exhibited a decreasing trend from inshore to offshore sites, with the higher values occurring in the coastal waters. The sea-to-air fluxes of C2H3Cl3, C2HCl3, CHCl3 and CCl4 were calculated to be-56.00–(-5.68),-7.31–123.42, 148.00–1 309.31 and-83.32–(-1.53) nmol/(m2·d), respectively, with the average values of-6.77, 17.14, 183.38 and-21.27 nmol/(m2·d). Our data showed that the SYS and ECS in autumn was a sink for C2H3Cl3 and CCl4, while it was a source for C2HCl3 and CHCl3 in the atmosphere. 相似文献